Objective: The aim of this study was to investigate the therapeutic effect and possible mechanism of essential oil extracted from Yinchenhao Tang Formula (essential oil extracted from Yinchenhao) on hepatic fibrosis model mice. Methods: Seventy Kunming mice were randomly divided into 7 groups (n=10): normal group, model group, colchicine group (0.2 mg/kg), essential oils extracted from Yinchenhao in different doses-low, medium and high dose group (2, 4, 8 g/kg) and autophagy inhibited group (3MA, 30 mg/kg). Except for the normal group, each group was treated with intraperitoneal injection of 10%CCl₄ corn oil to construct hepatic fibrosis mouse model twice a week for 8 weeks. In addition, each administration group was given the corresponding drug treatment once a day for 6 weeks. After the last dose, Hematoxylin-eosin (HE) staining was used to observe the pathological changes of the liver and kidney tissue in each group. The active levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured by the kit method, while the levels of liver fibrosis markers type Ⅳ collagen (Col-Ⅳ), type Ⅲ Procollagen (PC-Ⅲ), hyaluronic acid (HA), laminin (LN), malondialdehyde (MDA), and inflammatory cytokines including TNF-α, and IL-6 in the liver tissue were measured by ELISA. Besides, the immunohistochemistry method or western blot method was adopted to measure the expression levels of proteins in the liver tissue, including Smad3, TGF-β₁, LC3B and Beclin1. Results: Compared with the normal group, the liver tissue in the model group appeared significant symptoms: large amount of collagen fiber deposition, infiltration of inflammatory cell, unclear liver lobule structure, with obvious fibrous septum forming and liver cells degeneration and necrosis. Besides, it was found that while the active levels of ALT and AST in serum, the levels of Col-Ⅳ, HA, LN, PC-Ⅲ, MDA, LN (P<0.01), and the protein level of liver tissue TNF-ɑ and IL-6 increased significantly (P<0.01), Smad3 and TGF-β₁ levels in liver tissue (P<0.01) and LC3B and Beclin1 levels (P<0.05, P<0.01) increased dramatically. Moreover, comparing with the model group, it was found that the collagen deposition in liver of each dose essential oils groups were found significantly reduced, with the degree of fibrosis were alleviated in different levels and remarkably reduce in the levels of ALT and AST in serum, levels of Col-Ⅳ, HA, LN, PC-Ⅲ, MDA, LN (P<0.05, P<0.01), liver tissue TNF-ɑ and IL-6 levels were observed (P<0.05, P<0.01). In addition, liver tissue Smad3 and TGF-β₁ levels (P<0.05, P<0.01) were also found reduced significantly while the evident reduction in LC3B and Beclin1 levels (P<0.01) was found in oils extracted from Yinchenhao high dose group. Conclusion: Essential oil extracted from Yinchenhao Tang Formula have significant therapeutic effects on carbon tetrachloride (CCl₄)-induced mice with liver fibrosis model, which is able to obviously improve liver function, mitigate the liver fibrosis lesions. Through TGF-β₁/Smad3-mediated signal transduction pathway, the mechanism is able to reduce autophagy of liver cells fibrosis and realize the anti-fibrosis.

Objective: This study aimed to investigate the effect of EGFL9 on the proliferation and metastasis of hepatocellular carcinoma cells (HCC) in nude mice model. Methods: EGFL9 silenced JHH-7 HCC cell line was constructed by lentivirus-mediated small interfering RNA technology. The effect of EGFL9 on the proliferation of JHH-7 hepatoma cells was analyzed by MTT growth curve. The effect of EGFL9 gene on the proliferation and metastasis of JHH-7 liver cancer cells in vivo was observed by subcutaneous tumor formation experiment and tail vein injection metastasis experiment in nude mice. Results: The lentiviral infection efficiency of JHH-7^shEGFL9 cell group and JHH-7^shCtrl cell group was higher than 80% when observed under fluorescence microscope. The results of qRT-PCR assay demonstrated that the interference efficiency of EGFL9 in JHH-7^shEGFL9 cell group was 98.3%, indicating the JHH-7 human hepatoma cell line with EGFL9 silencing was successfully constructed. The results of MTT growth curve showed that the proliferation ability was significantly lower in the JHH-7^shEGFL9 cell group than in the JHH-7^shCtrl cell group (P<0.05). In nude mice with subcutaneous tumor inoculation, the mass [(0.246±0.104) g vs (0.710±0.221) g] and volume of subcutaneous tumors in the JHH-7^shEGFL9 group were significantly smaller than those in the JHH-7^shCtrl group (All P<0.05). After tail vein injection of tumor cells, the total fluorescence expression level in the mice of JHH-7^shEGFL9 group was significantly lower than that in the mice of JHH-7^shCtrl group [(2.75×10⁸±4.18×10⁸) vs (1.46×10⁹±1.30×10⁹), P<0.05], the incidence of lung metastasis (37.5% vs 62.5%) and the number of lung and kidney metastasis foci in the mice of JHH-7^shEGFL9 group were lower than those in the mice of JHH-7^shCtrl group (3 vs 6,14 vs 16), but the differences were not statistically significant (P>0.05). Conclusion: EGFL9 silencing significantly inhibited the proliferation and distant metastasis of HCC cells in nude mice, suggesting that EGFL9 may be a potential therapeutic target for HCC.

Objective: To investigate the inhibitory mechanism of dl-3-n-butylphthalide (NBP) on neuronal apoptosis after intracerebral hemorrhage (ICH) through the mitogen and stress activated protein kinase1-cAMP-response element binding protein(MSK1-CREB)signaling axis. Methods: A rat model of ICH was established using collagenase type Ⅶ injection into the caudate nucleus. Groups were set up as follows: control, sham operation(Sham), ICH+NBP and ICH+ normal saline (NS). Neurological function was assessed using the modified neurological severity score (mNSS). Brain water content was measured by the wet-dry weight method. Expression levels of Caspase-3, B-cell lymphoma-2(Bcl-2), cAMP-response element binding protein (CREB), and mitogen and stress activated protein kinase1 (MSK1) in the perihematoma tissue were detected by Western blotting and immunofluorescence analysis. Results: NBP treatment significantly improved neurological deficits after ICH in rats, alleviated brain edema, and inhibited the expression of Caspase-3 while increasing the expression of Bcl-2, CREB, and MSK1. Conclusion: NBP inhibits neuronal apoptosis after ICH in rats through the MSK1-CREB signaling axis, a theoretical basis for the application of NBP in the treatment of ICH.

Objective: This study aims to investigate the application effect of the “dual-track parallel” treatment process of the oculo-cerebral stroke green channel in superselective thrombolysis for patients with central retinal artery occlusion (CRAO). Methods: The “dual-track” treatment process was implemented, and 42 CRAO patients were selected as the control group, while 38 CRAO patients were selected as the observation group. After the two groups, the treatment response rate, macular foveal thickness, door-to-needle time (DNT), and key links were compared. Results: The treatment response rate was 23.7% higher than the control group, and there was a significant difference between the two groups (P<0.05). Compared with the control group, the observation group had a thinner macular foveal thickness one week after surgery, and the difference between the two groups was statistically significant (P<0.05). The median DNT was 220 minutes shorter than the control group, and there was a significant difference between the two groups (P<0.05). The observation group shortened the time from the medical orders to the fundus fluorescence angiography, the time to the return of the blood drawing, the time to the completion of the CT, the decision-making time, and the surgical arrangement time than that of the control group. There were significant differences between the two groups (P<0.01). Conclusion: The optimization of the treatment process by dual-track parallel implementing of the oculo-cerebral stroke green channel is effective, which can reduce the DNT and the time spent in critical points and improve the treatment response rate in patients with CRAO.

Objective: To improve the overall survival rate of LUAD patients through early screening and access to effective diagnosic and therapeutic tools, and to explore the impact of PIK3R1 gene hypomethylation on the diagnosis and prognosis of LUAD. Methods: Large public databases were used for analysis: TCGA, GEO, GEPIA2, UALCAN, Kaplan-Meier Plotter, LinkedOmics, and TIMER databases. Constructing univariate and multivariate COX proportional-hazards model in the clinical characteristics sub-groups of different LUAD populations. Results: The lower-expressed PIK3R1 significantly contributed to worse Overall Survival (OS), First Progression (FP) and Post Progression Survival (PPS) in LUAD patients. Subgroup for gender, tumor stage, smoking history, T classification, N classification and low expression of PIK3R1 were acting risk roles in LUAD. Lower expressed PIK3R1 was a potential independent risk factor in LUAD. DNA methylation analysis showed hypo-methylation of CpGs within the PIK3R1 promoter of the probe IDs (including cg01239651, cg24797508, cg16664523 and cg25008444) were significantly related to the low-expressed PIK3R1 through DNMT1. Receiver Operating Characteristic (ROC) curve analysis showed that PIK3R1 methylation and expression can be highly sensitive and specific in the accurate diagnosis of LUAD. Immunoassay study demonstrated that PIK3R1 hypomethylation and expression are associated with immune cells infiltration (B cells, CD8⁺T cells, CD4⁺T cells, macrophages, neutrophils and dendritic cells). Finally the co-expression of PGR, C5orf41, SCN7A, RBMS3, FAT4, RASSF2, A2M, ITGA8, FLI1 and RECK typically have similar functions with PIK3R1 were found. Conclusion: PIK3R1 hypomethylation and expression serve as a specifically diagnostic biomarker and independent prognostic factor that predicting immunotherapy effectiveness.

Objective: To analyze the risk factors of non-motor symptoms in patients with Parkinson’s disease (PD) with restless leg syndrome (RLS) and to investigate the related risk factors. Methods: Retrospectively collected 283 PD patients treated in the First Hospital of Hebei Medical University from October 2019 to December 2022, including 160 patients with RLS (PD-RLS) and 123 patients without RLS (PD-NRLS). By comparing the differences in mood (HAMD₂₄、 HAMA₁₄), cognition (MMSE), sleep (PQSI), fatigue (BFI), rapid eye movement (REM) sleep behavior disorder (RBD) motor symptoms (UPDRS-Ⅲ) and other factors between the RLS group and the non-RLS group. Logistic regression analysis was used to analyze the risk factors affecting RLS in PD patients. Results: ① The incidence of anxiety, depression, sleep disorders and RBD in PD-RLS was higher than that in PD-NRLS. ② Compared with the PD-NRLS, patients in PD-RLS were younger, but the HAMD₂₄, HAMA₁₄, PQSI, BFI, RBD and UPDRS-Ⅲ levels were higher (P<0.05). ③ In PD patients, RLS was not correlated with gender, Hoehn-Yahr stage and MMSE (P>0.05). ④ Multivariate Logistic regression analysis showed that the age and HAMA₁₄ were independent risk factors for RLS in PD patients. Conclusion: In PD patients, with the increase of age, the risk of RLS decreases, whereas the PD patients with anxiety should be alert to the occurrence of RLS. Early identification and treatment of RLS may improve the quality of life in PD patients.

Objective: To investigate the specific regulatory mechanism of human keratin Krt5 and M1/M2 macrophage polarization factor in the regeneration of jaw and heart bud base plasticity in zebrafish. Methods: Human Krt5 was overexpressed in zebrafish embryonic fibroblasts PAC2, followed by the addition of four representative macrophage polarization factor proteins. After detecting the protein expression, changes in the distribution of Krt5 and F-actin in PAC2 cells were observed by immunofluorescence; cell scratch assay was performed to detect the migration ability of PAC2 cells; transcriptome sequencing and immunoprecipitation-mass spectrometry (IP-MS) were used to analyze the Krt5 regulatory network. Results: Krt5 surrounded the nucleus and was distributed in bundles and networks in the cytoplasm and extended to the cytoplasmic membrane; overexpression of Krt5 increased the formation of cellular filamentous pseudopods represented by ghost pencil cyclic peptide and accelerated cell migration healing; transcriptome analysis showed that Krt5 significantly increased Rac2/Rhov, NF-κB, Mylkb, Ifnphi1, IL-13, and IL-11 transcription and decreased transcription of several collagen, actin and TGF-β family genes. IL-1β, IL-4, IL-6 and IL-10 induction treatments were generally consistent with Krt5 overexpressing effect; IP-MS confirmed that Krt5 tightly bound to α-Actinin (Actn1) and non-muscle myosin heavy chain (Myh9b, Myh10). Conclusion: Krt5 may activate Rax/Rho by interacting with actin to promote cellular filamentous pseudopods formation, thereby reshaping the interaction of cytokine receptor structures with macrophage polarization factors.

Objective: To conduct reliability and validity analysis on the Chinese version of the cognitive reserve index questionnaire (CRIq) to adapt it to the Chinese population. Methods: A total of 590 healthy volunteers (330 women and 260 men), mean age (39.71±9.81) years, mean years of education (15.34±3.23) years, were participated in the study. Thirty-six of the volunteers were assessed separately by two raters at different times to obtain inter-rater reliability. To obtain retest reliability, 34 of the volunteers were selected to be reassessed using the questionnaire 2~4 weeks after the first measurement. Independent samples t-test was used to observe gender differences. The validity of the scale was evaluated by exploratory factor analysis using the obtained data. Results: CRIq had a Cronbachs alpha coefficient of 0.835, and the reliability of the questionnaire was good. The inter-rater reliability was quite high (ICC: 0.998, 95% CI:0.997-0.999, n=36). Retest reliability results were good (r=0.805~0.996, n=34). Exploratory factor analysis extracted three factors (CRI-education, CRI-occupational activity, and CRI-leisure time) with a cumulative variance contribution of 46.17%. Conclusion: CRIq has good reliability and validity in the Chinese population.

Objective: To elucidate the molecular mechanism by which miR-9-5p regulates the proliferation, migration, and invasion abilities of colorectal cancer cells by targeting the expression of ZAK. Methods: The level of miR-9-5p and mRNA or protein expression levels of ZAK in colorectal cancer cell lines (HCT116, SW260, HT29 and LoVo) and clinical tissue samples were detected by qPCR and western blot. Bioinformatics analysis was employed to analyze the target sequences of miR-9-5p binding to ZAK. Dual-luciferase reporter assays were conducted to investigate the regulatory relationship between miR-9-5p and ZAK. Lipofection was used to co-transfect colorectal cancer cell lines HCT116 and SW620 with miR-9-5p mimics and/or ZAK plasmids. The effects of the miR-9-5p/ZAK signaling axis on cell proliferation were observed using CCK-8 and colony formation assays, while the effects on cell migration and invasion abilities were examined using Transwell assays. Results: Compared with human normal epithelial cell lines, the mRNA and protein levels of ZAK were significantly up-regulated in colorectal cancer cell lines (P<0.05), and ZAK protein level was up-regulated in colorectal cancer tissue samples (P<0.05). The expression level of miR-9-5p was down-regulated in cancer cell lines and tissue samples (P<0.05). Dual-luciferase reporter gene experiment showed that miR-9-5p significantly affected the luciferase activity of the wild-type ZAK 3’UTR expression vector (P<0.05). Overexpression of the miR-9-5p mimic fragment inhibited ZAK protein expression, while the inhibitor fragment promoted ZAK protein expression. CCK-8, colony formation and Transwell experiments demonstrated that overexpression of ZAK significantly promoted the growth, clony formation, migration and invasion ability of colorectal cancer cell lines (all P<0.05), while co-transfection of miR-9-5p counteracted the promoting effect of ZAK on cell function (all P<0.05). Conclusion: ZAK is highly expressed in colorectal cancer, while miR-9-5p is down-regulated; ZAK is a target gene regulated by miR-9-5p; The miR-9-5p/ZAK signaling axis collectively regulates the growth, migration and invasion of rectal cancer cells, involved in the occurrence and development of colorectal cancer.

Objective: To investigate the effect of Hanjingtang on phenotype transformation of aortic smooth muscle cells, and inflammation and oxidative stress in hypertensive rats of cold coagulation type, and to explore the mechanism of action. Methods: The rat model of cold coagulation type hypertension was established by cold stimulation combined with high salt diet for 6 weeks. The model rats were randomly divided into model(MOD) group, Hanjingtang (HJT) group and Candesartan(CCT) group, and a blank group(CON) was set as well. All groups were given corresponding drug intervention for 2 weeks. The blood pressure (BP) of rats was measured by small animal noninvasive blood pressure analysis system. HE staining and Masson staining was used for the analysis of aortic pathological change. Immunohistochemical assay was used for measure the vascular smooth muscle cell phenotype markers like α-SMA, OPN and calponin. Serum antioxidant markers SOD and MDA were measured by the WST-1 method and TBA, respectively. Serum inflammatory factors of IL-1β,TNF-α and NLRP3 were measured by Elisa. The protein expression levels of Nrf2, NQO1, NF-κB and IκBα were determined by immunoblotting in rat aorta. The gene expression levels of NQO1 and NF-κB were measured by Real-time fluorescence quantitative polymerase chain reaction assay in rat aorta. Results: Compared with the blank group, BP of the model group increased; the expression of SOD in serum decreased and the level of MDA, IL-1β, TNF-α and NLRP3 in serum increased; protein expression of Nrf2 and NQO1 decreased and protein expression of NF-κB and IκBα increased in rat aorta; NQO1 gene expression decreased and NF-κB gene expression increased in rat aorta. Compared with the model group, BP of rats in HJT group decreased, the expression of SOD in serum increased and the level of MDA, IL-1β, TNF-α and NLRP3 in serum decreased; protein expression level of Nrf2 and NQO1 increased and protein expression level of NF-κB and IκBα decreased in rat aorta; NQO1 gene expression level increased and NF-κB gene expression level decreased in rat aorta. The differences of the above indexes were statistically significant(P<0.05). Conclusion: The mechanism of HJT in reducing inflammation and oxidative stress, regulating vascular phenotype transformation and lowering BP in hypertensive rats of cold coagulation type is related to Nrf2/NF-κB signal pathway.

Objective: To investigate the correlation between mitochondrial damage and premature ovarian failure, as well as to explore the potential of repairing mitochondrial damage by umbilical cord mesenchymal stem cells for treating premature ovarian failure. Methods: Fifteen rabbits were randomly allocated into three groups, with three in the normal control group and six in each of the premature ovarian failure model group and umbilical cord mesenchymal stem cells treatment group. Both the model group and treatment group were intraperitoneally injected with 50 mg/kg of cyclophosphamide for two consecutive days to establish a POF model. In the treatment group, a suspension of 2 mL UCMSCs was administered via ear vein injection for three consecutive days starting from the third day after cyclophosphamide injection, with a cell count of 5×10⁶/mL. The model group was injected with the same volume of sterilization water. The serum levels of E2 and FSH were analyzed via venous blood samples prior to model construction, as well as at 1, 7 and 14 days post stem cell therapy. Ovarian tissue samples were collected on day of 14 days following treatment in order to observe the apoptosis of ovarian granulosa cells and changes in mitochondrial ultrastructure within the ovarian tissue.Additionally, relative expression of mtDNA was detected within the ovarian tissue. Results: ①After cyclophosphamide injection, the ovarian index of the model group exhibited a statistically significant decrease in comparison to the control group (P<0.05). ②Rabbits in the model group consistently exhibited low levels of E2 and high levels of FSH, with a statistically significant difference compared to the normal control group (P<0.05); In comparison to the model group, rabbits in the treatment group showed a significant increase in serum E2 levels and decrease in FSH levels (P<0.05). ③Compared to the normal control group, there was a significant difference in the number of apoptotic cells in the ovarian tissue of the model group (P<0.05); Additionally, compared to the model group, there was a significant difference in the number of apoptotic cells in the ovarian tissue of the umbilical cord mesenchymal stem cells treatment group (P<0.05). ④In the normal control group, follicular granulosa nuclei exhibited larger size and cytoplasm contained abundant round or oblong mitochondria that were scattered throughout. Conversely, in the model group, follicular granulosa cell mitochondria appeared disordered with swelling and vacuolation present. However, treatment with umbilical cord mesenchymal stem cells resulted in clear mitochondrial morphology within follicle granulosa cells along with some cristae presence and minimal vacuolation. ⑤Compared to the normal control group, the mtDNA content in the model group exhibited significant differences (P<0.05). Furthermore, compared to the model group, a statistically significant increase in mtDNA was observed in the ovarian tissue of the treatment group (P<0.05). Conclusion: Mitochondrial damage repair may potentially contribute to the therapeutic effect of umbilical cord mesenchymal stem cell treatment for premature ovarian failure.

Objective: To explore the application value of ALP, tumor markers (CEA, CA125, CA153, CYFRA21-1) and immune-inflammatory indicators(NLR, PLR, LMR, SII, SIRI) in predicting the risk of breast cancer bone metastasis. Methods: A total of 150 patients with breast cancer who were newly diagnosed at Sun Yat-sen Memorial Hospital of Sun Yat-sen University from October 2016 to October 2020 were included,including 50 patients with bone metastasis and 100 patients without bone metastasis.Clinical data and test results of serum ALP, serum tumor markers (CEA, CA125, CA153, CYFRA21-1), and peripheral blood immune-inflammatory indicators (NLR, PLR, LMR, SII, SIRI) were collected before treatment. The differences in clinical data and various indicators were compared between patients with and without bone metastasis.Spearman correlation analysis was conducted to evaluate the correlation between each indicator and the grade of bone metastasis in breast cancer.Logistic regression analysis was performed to identify effective indicators for predicting bone metastasis in breast cancer,and the predictive value of each indicator was assessed using ROC curves. Results: There were significant statistical differences (P<0.05) in histological classification, tumor size, lymph node metastasis, and visceral metastasis between the breast cancer patients with and without bone metastasis. The levels of ALP, CEA, CA125, CA153, CYFRA21-1, NLR, PLR, SII and SIRI in the breast cancer bone metastasis group were significantly higher than those in the non-bone metastasis group, while the level of LMR was significantly lower than that in the non-bone metastasis group, and all differences were statistically significant(P<0.05).The levels of ALP, CEA, CA125, CA153, CYFRA21-1, NLR, PLR, SII, and SIRI were positively correlated with the grade of bone metastasis in breast cancer (P<0.05). Conversely, the level of LMR was negatively correlated with the grade of bone metastasis in breast cancer (P<0.05).Visceral metastasis, elevated levels of ALP, CEA, CYFRA21-1, and NLR were identified as risk factors for bone metastasis in breast cancer.The areas under the ROC curves of ALP, CEA,CYFRA21-1 and NLR were 0.824,0.746,0.845 and 0.718, respectively. The combined detection of these four indicators had an AUC of 0.915, which was higher than any individual indicator, with a sensitivity and specificity of 78.0% and 89.0%, respectively. Conclusion: The combined detection of serum ALP, CEA, CYFRA21-1, and peripheral blood NLR has significant application value in predicting the risk of breast cancer bone metastasis,which can aid in the early detection and evaluation of bone metastasis in breast cancer patients.

Breast cancer with low expression of Human Epidermal Growth Factor Receptor 2 (HER-2) was defined by Immunocytochemistry score 1+ or 2+ and In Situ Hybridization negative. The previous binary classification of HER-2 patients and the limitations of traditional HER-2 targeted drugs have resulted in this group of people not receiving much attention. With the revelation of Destiny-Break04 (DB-04) research results, the effect of antibody drug conjugate (ADC) (DS-8201) on patients with advanced breast cancer with low HER-2 expression has been affirmed. It provides more possibilities for the treatment and prognosis of breast cancer patients with low expression of HER-2. Although the concept of HER-2 low expression is widely used, existing evaluation standards and detection methods have certain limitations in accurately identifying such patients. Whether breast cancer patiemts with low expression of HER-2 can become an independent biological entity and the significance of HER-2 evaluation indicators in guiding prognosis and treatment is still controversial. The prognosis of early breast cancer patients with low HER-2 expression has also attracted much attention. Most of the previous studies were retrospective, and the results were inconsistent, It’s worth exploring further. This article systematically reviewed the clinicopathological characteristics, response to systematic treatment, prognosis and future exploration direction of early breast cancer with low HER-2 expression.

Objective: To investigate the expression of circular RNA_0053063 (circ_0053063) in colon cancer cells and its effect on the migration and invasion ability of colon cancer cells and the regulatory mechanism. Methods: The expression levels of circ_0053063 in normal colonic epithelial NCM460 cells and colon cancer LoVo, HCT116, SW480, CACO-2 and HT29 cells were detected by qRT-PCR. Colon cancer LoVo cells stably overexpressing circ_0053063 were constructed. Transient transfection of shATG13 was applied to interfere with the expression of ATG13. The migration and invasion ability of each group of LoVo colon cancer cells were detected by wound healing assay and Transwell assay after pretreatment with lipopolysaccharide. Results: circ_0053063 was highly expressed in normal colonic epithelial NCM460 cells, while it was lowly expressed in colon cancer LoVo, HCT116, SW480, CACO-2 and HT29 cells. Stable overexpression of circ_0053063 significantly promoted the migration and invasive ability of colon cancer LoVo cells. Transient interference with ATG13 inhibited the migratory and invasive ability of colon cancer LoVo cells caused by overexpression of circ_0053063. Conclusion: Circ_0053063 regulates the migration and invasion ability through an ATG13-dependent pathway in colon cancer cells.

Objective: To examine the expression, clinical value and biological function and the underlying mechanism of AAMDC in hepatocellular carcinoma (HCC). Methods: The correlation between the expression abundance of AAMDC in HCC and its clinicopathological characteristics and prognosis were examined by immunohistochemistry and tumor databases. qRT-PCR and Western blot were used to detected the mRNA and protein expression levels of AAMDC. The impact of AAMDC on the migration and invasion were determined by transwell. The cellular localization of AAMDC was examined by immunofluorescence. The interaction network and biological function was investigated by Co-IP and mass spectrometry, and the downstream pathways of AAMDC in HCC were revealed by transcriptomics. Results: The expression of AAMDC was upregulated in HCC tissues and hepatoma cell lines. High AAMDC expression was significantly associated with poor disease-free survival. AAMDC was mainly distributed in the cytoplasm of HCC. Overexpression of AAMDC enhanced, whereas knockdown of AAMDC inhibited the migration and invasion of HCC cells. Co-IP and mass spectrometry demonstrated that AAMDC interacted with fatty acid synthetase (FASN). High-throughput transcriptomics and in vitro experiments showed that overexpression of AAMDC activated the mTOR pathway. Conclusion: AAMDC is up-regulated in HCC, and exerts oncogenic function to promote cell invasion and migration via the interaction with FASN to activate mTOR signaling.

Objective: The study aimed to create a personalized predictive model for postoperative recurrent lumbar disc herniation (PRLDH) following percutaneous endoscopic transforaminal discectomy (PETD) in patients. Methods: Data from 227 patients who underwent PETD surgery between June 2019 and January 2023, including 28 additional clinical features were collected. Independent risk factors for PRLDH were identified using Lasso and multiple-factor logistic regression, leading to the development of a Nomogram model. Internal validation was performed to assess model accuracy, calibration, and clinical utility. Results: Intervertebral disc angles, functional cross-sectional area of the ipsilesional multifidus muscle, Modic changes, and BMI were identified as independent predictive factors significantly associated with the occurrence of PRLDH (P<0.05). A Nomogram model was developed based on selected predictors. Internal validation resulted in an AUC of 0.867 (95%CI: 0.787~0.946) for predicting PRLDH. The Hosmer-Lemeshow test yielded non-significance ( x²=10.154, P=0.254), and the calibration curve showed good consistency between observed and predicted values. DCA identified a threshold range of 0.05~0.91 for PRLDH prediction, indicating clinical net benefits. CIC indicated that within this threshold probability range, the expected recurrent cases exceeded the actual ones. Furthermore, the Nomogram model consistently outperformed individual risk factors in clinical benefits. Conclusion: The Nomogram predictive model, based on the assessment of paraspinal muscles, demonstrates excellent predictive capability and contributes to the personalized assessment of the risk of PRLDH.

Objective: To investigate the technical characteristics and therapeutic effects of transanterolateral approach microsurgery for the treatment of cervical paraneurogenic tumors. Methods: The clinical data of 15 patients with cervical paravertebral neurogenic tumors treated by anterolateral approach from June 2017 to September 2021 in the Department of Neurosurgery, the First Affiliated Hospital of Jinan University were summarized and analyzed. The incision length, operation time, blood loss, total tumor resection rate, and postoperative complications of the patients were analyzed. Results: The operation time ranged from 62 to 143 min, the blood loss was 50 to 120 mL, the surgical incision length was 7 to 12 cm, and the hospital stay was 7 to 9 days. The paravertebral tumor resection rate was 100%. Pathological diagnosis was made in 9 cases of neurofibroma, 5 cases of schwannoma and 1 case of paraganglioma. Follow-up for 12 months showed no tumor recurrence, infection or death. Conclusion: The anterolateral approach is safe and effective for the treatment of cervical side neurogenic tumors.

Objective: To construct a post competency evaluation index system for nurses in the hybrid operating room, in an attempt to provide reference for post setting, ability training, and assessment of nurses in the hybrid operating room. Methods: A post competency evaluation index system was constructed for nurses in the hybrid operating room based on literature retrieval, semi-structured interview, and Delphi method, and the analytic hierarchy process was used to determine the indicator weights. Results: The effective recovery rate of the questionnaire reached 97%, 100% after two rounds of consultation. Besides, the importance scores of all indexes were (4.12±0.78)~(4.94±0.24), the coefficient of variation (CV) was 0.05~0.19, the authority coefficient was 0.81~0.90, the Kendall’s coefficient of concordance was 0.31~0.33, and the consistency test result was P<0.001, the index weight was 0.0028~0.2987. The post competency evaluation index system for nurses in the hybrid operating room included 7 primary indexes (hybrid operation expertise, hybrid operation nursing skills, communication & coordination ability, organization & management ability, professional quality, personal characteristics, and career planning) and 36 secondary indexes. Conclusion: The post competency evaluation index system for nurses in the hybrid operating room has high reliability, scientificity and specialty. It may provide a theoretical foundation for the selection, training, and assessment of nurses in the hybrid operating room.

One of the common RNA post-transcriptional changes, 5-methylcytosine (m⁵C) methylation, is controlled by the cooperative action of m⁵C methyltransferases and binding proteins. Tumor growth is influenced by the abnormal expression of RNA m⁵C, which is directly linked to cancers of the digestive system. Specific cancer cells are encouraged to proliferate, migrate and invade as a result of the elevated level of m⁵C. In digestive system cancers, it is envisaged that RNA m⁵C methylation and its regulatory mechanism may become novel biomarkers, providing potential targets for intervention, prevention and treatment.

Objective: To study the weighting of MRI images of lumbar disc herniation in order to develop an optimal and uniform diagnostic imaging pathway. Methods: After reading the literature, we extracted statements, keywords and related words about the diagnosis of MRI images of lumbar disc herniation to establish a pool of entries, discussed the entries by brainstorming method, constructed a questionnaire by combining the discussed entries with Likert’s five levels of importance, conducted expert consultation using the Delphi method, and calculated the weight coefficients of MRI images of lumbar disc herniation based on the consultation results and the diagnostic imaging pathway is formed by ordering the weight coefficients. Results: The following diagnostic imaging pathways were developed: nerve root compression (LNRC), nucleus pulposus moisture (LNPM), disc morphology and height (LDMAH), cerebrospinal fluid patency (CFP), cauda equina travel (CEL), fibrous ring integrity (LDFRI), posterior longitudinal ligament integrity (PLLI), nucleus pulposus-fibrous ring demarcation (NPAFRD), vertebral body stability (LVBS), endplate morphology (LEM), osteophytes (LVO), Modic changes (MC), and Schmorl’s nodes (SN). Conclusion: The MRI imaging diagnostic pathway of lumbar disc herniation explored in this study can provide the basic conditions for later imaging intelligent diagnosis and reference for related imaging studies.

Objective: To develop an artificial intelligence (AI) algorithms tool for data collection in chronic hepatitis B, addressing the issue of low efficiency in traditional multicenter data collection. Methods: Based on internationally recognized data standards, this study applies mature AI techniques in the field of computer science, such as optical character recognition and natural language processing, to the data collection of real-world cohorts in chronic hepatitis B research. This tool enables the electronic collection of various data formats, including digitization of raw data in image format, structured processing, and automatic population of data into electronic case report forms (eCRFs) designed in the Research Electronic Data Capture (REDCap) system. Results: Experimental results demonstrate that the AI algorithmstool for data collection achieves the same average accuracy as manual data collection (P=0.23), with an accuracy rate of 98.66%, while reducing the time required by 75.49%(P<0.05) compared to manual collection. Conclusion: The AI algorithms tool for data collection developed in this study significantly improves the efficiency of research data collection and brings new developments to the real-world research data collection paradigm.

Objective: To explore the application value of shear wave elastography (SWE) combined with ultrasound weighted score in the diagnosis of breast lumps. Methods: A total of 120 patients with breast mass admitted to our hospital from May 2018 to May 2020 were retrospectively selected as research objects, including 62 cases with benign lesions in benign group and 58 cases with malignant lesions in malignant group. All underwent routine breast ultrasound examination and SWE. The maximum SWE, minimum SWE, mean SWE and ultrasound weighted score of the two groups were compared, and ROC curve was used to analyze the diagnostic efficiency of shear wave elastic imaging score combined with ultrasound weighted score in the diagnosis of breast mass. Results: The ultrasound weighted score of the malignant group was (7.97±4.01) points, which was significantly higher than that of the benign group (4.48±2.34) points (t=5.771, P<0.05). There was no significant difference between ultrasound weighted score and SWE combined ultrasound weighted score in the diagnosis of breast lumps (P>0.05). The maximum and mean values of SWE in benign group were lower than those in malignant group (P<0.05). There was no significant difference in the minimum value of SWE between the two groups (P>0.05). Binary Logistic regression analysis showed that the maximum value of SWE, the average value of SWE and the weighted score of ultrasound were the factors affecting benign and malignant breast masses (P<0.05). ROC curve analysis showed that the AUC of maximum SWE, mean SWE and ultrasound-weighted score was 0.931, higher than that of ultrasound-weighted score (0.763), maximum SWE (0.799) and mean SWE (0.769) (Z=4.188; Z=3.241; Z=3.938, P<0.05). Conclusion: Shear wave elastic imaging score combined with weighted ultrasound score can improve the accuracy of breast mass diagnosis and contribute to the early screening and diagnosis of breast cancer.

Objective: To investigate whether brain-derived neurotrophic factor (BDNF) is involved in protection of the survival of microvascular endothelial cells (MECs) under glucose starvation. Methods: The glucose starvation model was established using mouse brain microvascular endothelial cell line, bEnd.3. The modeled MECs were treated with siRNA of BDNF and BDNF-TrkB pathway blockers respectively. The flow cytometry was applied to investigate the apoptosis of treated cells. Western blot and qRT-PCR were used to analyze the expression of BDNF and other regulatory genes. Results: Glucose starvation leaded to apoptosis of MECs, and the degree of late apoptosis was increased with the prolongation of glucose starvation. With glucose starvation, the expression of BDNF in MECs was up-regulated. Under glucose starvation, when BDNF-TrkB pathway was blocked with inhibitor of BDNF receptor, K252a, the early apoptosis of MECs was increased. In addition, inhibition of BDNF expression was able to increase apoptosis of MECs in glucose starvation. Conclusion: BDNF is involved in protection of the apoptosis of MECs mediated by glucose starvation, and in the maintenance of the expression of PFKM gene, a key regulatory gene of glycolysis, in the non-glucose starvation and glucose starvation. Thus plays an important regulatory role in maintaining the survival of MECs and energy supporting of MECs via glycolysis.

Studies have found a strong relationship between chronic periodontal disease and cardiovascular disease (CVD). Porphyromonas gingivalis is a common bacterium in periodontal disease with a detection rate of 100% in cardiovascular plaques. In this paper, the pathogenic factors of Porphyromonas gingivalis were summarized: fimbriae, gingival protease, capsula, etc. To explore the correlation between Porphyromonas gingivalis and CVD, Porphyromonas gingivalis on the pathogenic pathways of CVD, including phagocytes-mediated and bacteremic infection, and the pathogenic mechanism of Porphyromonas gingivalis on CVD, including oxidative stress (OS), endothelial dysfunction, and vascular remodeling, aiming to provide ideas for later studies of the occurrence and development of CVD associated with Porphyromonas gingivalis.

Objective: To investigate the clinical effect of low-dose aspirin (LDA) plus different doses of calcium in the prevention of preeclampsia and its influence on inflammatory factors. Methods: According to the method of random number table,the patients were randomly divided into control group, LDA group, LDA+low-dose calcium and LDA+high-dose calcium group. And the maternal and infant outcomes, coagulation function, and inflammatory factors in each group were compared. Results: The incidence of severe preeclampsia in the LDA + low-dose calcium group and the LDA + high-dose calcium group was significantly lower than that in the control group and the LDA group (P<0.05). Compared with the control group and LDA group, the incidence of premature delivery, FGR, fetal distress and cesarean section in the LDA+low-dose cacium group and LDA+high-dose calcium group were significantly reduced, while the gestational weeks of delivery, weight and length of newborns were significantly increased, the difference was statistically significant (P<0.05). Compared with the LDA group, the weight of newborns in the LDA+low-dose calcium group was significantly increased, while the gestational week, newborn weight, length, and 1-minute Apgar score of the LDA+high-dose calcium group were significantly increased, the difference was statistically significant (P<0.05). After treatment, compared with the control group and LDA group, PT, APTT and TT in LDA+low-dose calcium group and LDA+high-dose calcium group were significantly prolonged, while Fib and TNF-α, IL-6 and hs-CRP decreased significantly (P<0.05). Conclusion: Aspirin combined with calcium can improve the blood coagulation and inflammatory function, which has a certain role in preventing preeclampsia and improving the maternal and infant outcomes, and is worthy of clinical promotion.

The Ocimum plants are annual or perennial herbs or shrubs, which are used in medical researches or treated as ornamental plants. Their chemical constituents include terpenoids, flavonoids, phenylpropanoids etc, which have significant pharmacological effect on anti-tumor, anti-diabetic, anti-microorganism, anti-inflammation, anti-depression and anti-amnesic properties, anti-gastrointestinal diseases,and relief of pain. This paper summarized the chemical constituents and pharmacological activities of the main medicinal plants from Ocimum, which can be a good reference for further studies and utilization of the Ocimum plants.

Objective: To explore the effects of Casticin on the expression of miR-342-3p and glycolysis in cervical cancer HeLa cells, and to test whether Casticin inhibited glycolysis in cervical cancer HeLa cells by upregulating the expression of miR-342-3p. Methods: The method employed in the study was to treat HeLa cells with different concentrations (0, 10, 30, 100 nmol/L) of Casticin and Casticin (30 nmol/L) combined with miR-342-3p mimics or inhibitor transfection. RT-qPCR assay was adopted to examine the expression level of miR-342-3p in HeLa cells. Simultaneously, through measuring relative glucose consumption and relative lactate production, the effects of Casticin or Casticin combined with miR-342-3p mimics or inhibitor transfection on the glycolysis in cervical cancer HeLa cells were detected and analyzed. Results: Casticin upregulated the expression level of miR-342-3p in a concentration dependent manner as well as reduced the glucose consumption and inhibited the lactate production in HeLa cells. Moerover, miR-342-3p mimic synergistically inhibits glucose consumption and lactate production in HeLa cells in combination with Casticin. On the contrary, miR-342-3p inhibitors could rescue Casticin’s upregulation of miR-342-3p expression level as well as the inhibitory effects on glucose consumption and lactate production of HeLa cells. Conclusion: The conclusion indicated that Casticin inhibited glycolysis in HeLa cells through upregulating the expression level of miR-342-3p.

Objective: To investigate the clinical characteristics and risk factors of extubation in patients with tracheotomy after stroke. Methods: The clinical data of 72 patients with tracheotomy after stroke who were hospitalized in the Department of Hyperbaric Oxygen Rehabilitation, General Hospital of Southern Theater Command from 2015 to 2023 were retrospectively analyzed. Binary logistic regression method was used to analyze the factors that may affect extubation. Results: Univariate analysis showed that age, NHISS score at admission, NHISS score at discharge,Stroke type,and consciousness state at discharge were correlated with extubation. Logistic regressionanalysis showed that NHISS score and consciousness state at discharge score was an independent factor for extubation. The x² value of the Hosmer-Lemeshow test was 8.232(P=0.411), and Area Under Curve (AUC) of ROC is 0.916 suggesting that the goodness of fit of this model is outstanding ( x²=0.9166). Conclusion: The ability of extubation in patients with tracheotomy after stroke is related to age, Stroke type,NHISS score and state of consciousness.

Objective: The study aimed to predict the 2017~2021 survival rate of patients with Hepatocellular carcinoma (HCC) in the United States through the Surveillance, Epidemiology, and End Results (SEER) database. Methods: Data were provided by the SEER database. The period analysis to collate and evaluate hepatocellular carcinoma data from 2002 to 2016 in the SEER database were performed and predicted 3- and 5-year survival rates in the subsequent 5 years. Results: The relative survival rates showed an overall upward trend from 2002 to 2016, among which the 3-year and 5-year survival rates of HCC patients from 2002~2006 were 36.4% and 26.9% respectively, and the 3-year and 5-year survival rates of HCC patients from 2007~2011 were 39.9% and 30.3% respectively, and the 3- and 5-year survival rates of HCC patients from 2012~2016 were 48.6% and 38.1% respectively. The expected 3- and 5-year survival rates from 2017 to 2021 are 49.40% and 43.55% and show obvious upward trends compared to 2002~2016. The trends of the relative survival rates differed according to age group, sex, race, marital status, and surgery treatment. Conclusion: Period analysis provides an effective prediction of survival rate from 2017 to 2021. Understanding the 3- and 5-year survival rates of HCC patients from 2002 to 2016 and the expected survival rates in the subsequent 5 years is of great significance to the development of strategies for the treatment of hepatocellular carcinoma treatment and the reduction of its morbidity and mortality.

Objective: To evaluate the clinical efficacy of cervical discectomy by unilateral biportal endoscopy(UBE) for cervical spondylotic radiculopathy. Methods: Data of 7 patients with cervical spondylotic radiculopathy who underwent cervical discectomy via UBE from April 2021 to November 2022 were reviewed. Visual analogue scale(VAS), neck disability index(NDI)were recorded to evaluate the clinical outcome. The excellent and good rate of operation was evaluated by using MacNab criteria. The cervical curvature and the surgical segment ROM were compared pre- and post-operation. Results: The mean operation time was (120.0±39.7)min, the mean intraoperative blood loss was (24.3±14.0)mL, the mean postoperative hospitalization time was (4.3±0.8)days. The mean preoperative VAS decreased from (6.7±0.8) to (1.7±0.5) at the last postoperative follow-up, and the mean preoperative NDI decreased from (59.7±7.0)% to (10.7±1.7)% at the last postoperative follow-up (P<0.05). There was no significant difference in cervical curvature and operative segment ROM at 1 month after surgery and at the last follow-up (P>0.05). There was no significant difference in cervical curvature and surgical segment ROM preoperatively and postoperatively(P>0.05). The excellent and good rate was 100% according to MacNab criteria (excellent in 4 cases, good in 3 cases. All the 7 patients were successfully operated without cerebrospinal fluid leakage, muscle strength loss, operative infection, epidural hematoma and other serious complications. Conclusion: UBE sliding technique is an effective and safe operation for treatment of two-level CSR.

Objective: To investigate effects of partial depolarization on peripatellar tissues underwent electrocautery on the post-operative knee joint and patellar functions of patients underwent unicompartmental knee arthroplasty (UKA) surgery. Methods: This retrospective study was conducted based on 60 knee osteoarthritis (OA) patients with UKA surgery in the Affiliated Chizhou Hospital of Wannan Medical College from March 2020 to October 2022. Patients were randomly assigned to the treatment group and control group. Patients in the control group received only UKA surgery, while patients in the treatment group received UKA surgery combined with partial depolarization of peripatellar tissue underwent electrocautery. Pain, knee joint function, patellar inclination angle and the Feller patellar score of the both groups were compared before and 1, 3, 6 months after surgery, respectively. Results: The Visual Analogue Scale scores of patients in the treatment group were significantly lower than the control group at 1 month (t=3.437, P=0.001), 3 months (t=6.591, P<0.001) and 6 months (t=5.162, P<0.001) after surgery, respectively. The Hospital for Special Surgery Knee scores of patients in the treatment group were higher than the control group at 1 month (t=9.625, P<0.001), 3 months (t=7.970, P<0.001) and 6 months (t=4.092, P<0.001) after surgery, respectively. The patellar inclination angles of patients in the study group were significantly smaller than the control group at 1 month (t=2.571, P=0.013), 3 months (t=2.671, P=0.010) and 6 months (t=2.741, P=0.008) after surgery, respectively. Feller scores of patients in the study group were higher than the control group at 1 month (t=2.510, P=0.015), 3 months (t=3.384, P=0.001) and 6 months (t=3.483, P=0.001) after surgery, respectively. Conclusion: Partial depolarization of peripatellar tissues by electrocautery can effectively improve the knee and patellar functions in OA patients after unicompartmental knee arthroplasty surgery.

Objective: The aim of this study was to investigate the predictive value of blood lactate, interleukin-6 (IL-6), and the sequential organ failure assessment (SOFA) score on the 28-day mortality risk in sepsis patients. Methods: Retrospective analysis of clinical data of 145 sepsis patients admitted to the Department of Critical Care Medicine at the Second Affiliated Hospital of Xinjiang Medical University. All patients were admitted to the hospital and laboratory tests such as blood gas analysis and routine blood tests were completed and SOFA scores were performed. All patients were followed up for 28 days. Based on survival outcome, the patients were divided into two groups: the survival group (46 cases) and the death group (99 cases). Comparative analysis was then carried out to assess the differences in blood lactate, IL-6, and SOFA scores between the two groups. Univariate and multivariate logistic regression analyses were performed to identify independent factors influencing sepsis patient mortality. Spearman correlation analysis was employed to examine the associations between blood lactate, IL-6, SOFA scores, and sepsis patient mortality. Furthermore, the predictive value of blood lactate, IL-6, and SOFA scores, as well as their combined tests, were evaluated using receiver operating characteristic (ROC) curves. The model underwent 500 resampling iterations using the Bootstrap method in R, followed by the generation of calibration curves and clinical decision curves.Results: Blood lactate levels, IL-6 and SOFA scores of patients in the sepsis death group were significantly higher than those in the survival group, and the differences were all statistically significant (P<0.05). The overall morbidity and mortality rate among patients with sepsis was 68% (99/145). Univariate logistic regression analysis shows diabetes, blood lactate, IL-6, and SOFA score as correlates of death in patients with sepsis. Multivariable logistic regression analysis revealed that high SOFA score, having diabetes mellitus, and high IL-6 were independent risk factors for death in sepsis patients. Furthermore, blood lactate, IL-6, and SOFA scores positively correlated with sepsis patient mortality, while the area under the ROC curve (AUC) for blood lactate, IL-6, SOFA scores, and the combination of the three tests were 0.863, 0.850, 0.897, and 0.949, respectively. The optimal threshold values for lactate, IL-6, and SOFA scores were identified as 2.950 mmol/L, 100.970 pg/mL, and 7.500 points, respectively. The diagnostic sensitivity and specificity for lactate were 77.800% and 89.100%, for IL-6 were 70.700% and 91.300%, and for SOFA score were 93.900% and 80.400%. The combined test exhibited a sensitivity of 90.900% and a specificity of 89.100%. Conclusion: Elevated levels of blood lactate, IL-6, and SOFA scores have been identified as independent risk factors for mortality in patients with sepsis. The clinical prediction model developed in this study demonstrates high accuracy and clinical applicability, while the simultaneous use of these three tests holds significant predictive value in prognostic analysis for sepsis patients.

Objective: To evaluate the application value of deep learning-based reconstruction(DLIR) algorithm in superior mesenteric artery CT angiography. Methods: The 40 patients’ scan raw data of CT angiography of superior mesenteric artery were retrospectively included in this study. The images were reconstructed using six algorithms, including filtered back projection (FBP), adaptive statistical iterative reconstruction (ASIR)30% and 60%, deep learning image reconstruction (DLIR)-L, DLIR-M and DLIR-H. The CT value, SD value and signal to noise ratio of superior mesenteric artery were analyzed and two radiologists evaluated the subjective image quality. Results: There was a statistical difference (P<0.001) between the six algorithms in terms of CT value, noise, signal-to-noise ratio (SNR), and subjective image quality score for reconstructed images. Specifically, DLIR-H, DLIR-M, ASIR60%, DLIR-L, ASIR30%, and FBP images showed an increase in noise and a decrease in signal-to-noise ratio. There was no significant difference in CT values among the three types of DLIR, but the CT values of DLIR were higher than FBP and ASIR. Subjective scoring showed enhanced vascular sharpness in DLIR reconstructed images. Conclusion: DLIR can improve the image quality of CT angiography of superior mesenteric artery and improve the vessel sharpness, but there is also a risk of changing the original information.

Objective: To systematically evaluate the risk factors of knee osteoarthritis. Methods: We searched Wanfang, CNKI, VIP, Web of Science, PubMed, Cochrane and other databases by computer from the beginning of the database establishment to June 2022 to collect the literatures about the risk factors of knee osteoarthritis. Data were independently screened and extracted by 2 individuals for quality control, and a meta-analysis was performed using Cochrane RevMan 5.4 software. Results: A total of 12 Case-control study literatures were included in this study for quantitative analysis evaluation.Meta-analysis showed that age (OR=1.08,95% CI[1.06,1.11]), female (OR=2.67,95% CI[2.18,3.27]), BMI (OR=2.03,95% CI[1.50,2.73]), high-intensity exercise (OR=1.89,95% CI[1.52.2.35]), frequent squatting or kneeling (OR=3.87,95% CI[1.09,13.77]), smoking (OR=1.33,95% CI[1.24,1.43]), alcohol consumption (OR=2.28,95% CI[1.42,3.66]), history of other knee joint diseases (OR=2.63,95% CI[1.36,5.08]) and family history of knee osteoarthritis (OR=1.69,95% CI[1.36,2.11]) were the risk factors of knee osteoarthritis, while education level was not associated with knee osteoarthritis. Conclusion: In order to reduce or delay the occurrence of knee osteoarthritis, smoking cessation, alcohol abstinence, reduction of squatting or kneeling movements, weight control and moderate to low-intensity exercise should be advocated in daily life. Patients with a history of knee joint trauma and family history of knee osteoarthritis should pay attention to protect the knee joint and prevent the occurrence of knee osteoarthritis.

Objective: To create an evidence-based dietary plan and assess its effectiveness for patients who undergo laparoscopic sleeve gastrectomy surgery. Methods: A systematic search of seven English databases (Cochrane Library, EMBASE, Medline, Scopus, PubMed, Web of Science, and BMJ Best Practice) and four Chinese databases (CNKI, Wanfang Data, VIP, and SinoMed) was conducted to identify postoperative diet research for LSG patients. The study involved synthesizing data from systematic evaluations, meta-analyses, systematic reviews, and papers to develop an evidence-based dietary management program tailored to postoperative LSG patients. Using a convenience sampling method, patients who underwent LSG at Guangdong Provincial People’s Hospital between January and December 2022 were recruited. The recruited patients were then randomly assigned to two groups. In the observation group, 36 participants adhered to an evidence-based dietary regimen, while the control group consisted of 35 participants following a traditional dietary regimen. Measurements of waist circumference, hip circumference, waist-to-hip ratio, and body mass index (BMI) were taken and statistically analyzed prior to and at 1, 3, 6, 9, and 12 months following LSG surgery for all participants. Results: Without considering the passage of time, a statistical analysis of variance between the observation and control groups showed a statistically significant difference in the primary effects of the intervention on waist circumference and waist-to-hip ratio (P<0.05). Over time, there were significant differences in the primary effects of the intervention on waist circumference, hip circumference, waist-to-hip ratio, and BMI (P<0.05). There was a statistically significant interaction effect between the intervention and the passage of time (P<0.05). Starting from the 6th month post-operation, there were significant differences in waist circumference, hip circumference, waist-to-hip ratio, and BMI between the observation group and control group based on intergroup comparisons at various time points (P<0.05). Furthermore, the group under observation demonstrated a considerable reduction in each metric when compared to the control group. Conclusion: In conclusion, a multidisciplinary team’s evidence-based dietary management program and long-term follow-up intervention can effectively maintain the patients’ nutritional status and therapeutic outcomes after undergoing LSG.

Objective: To investigate the efficacy and safety of lidocaine aerosol through gastroscopy in patients with obstructive sleep apnea syndrome (OSAS). Methods: A total of 100 OSAS patients aged from 24 to 60 years were enrolled to the study from patients admitted to the gastroscopy center of our hospital from July 2022 to December 2022. Patients with ASA grade of Ⅱ~Ⅲ and STOP-Bang scale score of ≥3 points were arranged for analgesic gastroscopy. Patients were divided into group A and group B through random number table method (n=50). Patients in group A was pretreated with lidocaine aerosol throat surface anesthesia, followed by remifentanil+propofol combined intravenous anesthesia, while group B was treated with remifentanil+propofol combined intravenous anesthesia separately. Ramsay sedation score, anesthetic effect during the examination, dosage of propofol at the time of successful gastroscopy implantation and total dosage of propofol after the examination, number of cases of respiratory depression (SpO₂<90%) during the examination, number of cases of cough during the examination, and number of cases of endoscopist suspending the operation during the examination were recorded as evaluation indicators in the two groups. Results: Compared with group B, Ramsay’s sedation score and anesthetic effect were significantly decreased in group A after gastroscopy implantation. Besides, reduced number of cases of respiratory depression during the examination, decreased total dosage of propofol during the examination, reduced number of cases of choking during the examination, and the number of cases of suspension during the examination were observed in patients of group A. Conclusion: Lidocaine aerosol significantly reduces and the adverse reactions in intravenous anesthesia, especially the occurrence of respiratory depression, and the dosage of propofol in OSAS patients which further improves the anesthetic effect and the safety of anesthesia. Thus, lidocaine aerosol is especially suitable for painless gastroscopy anesthesia in OSAS patients.

Oral implant restoration for patients with severe skeletal class Ⅲ edentulous jaws is challenging and requires multidisciplinary treatment. However, with the development of oral implant technology, it is possible to restore severely skeletal class Ⅲ edentulous jaws with implant-supported fixed dentures. There are few reports at home and abroad about implant restoration in such cases. In this paper, a case of severe skeletal class Ⅲ edentulous was restored by implant fixed dentures, and the diagnosis and treatment process and postoperative clinical efficacy were evaluated, with the view to provide a certain reference for the clinical treatment of such patients.

Objective: To investigate the incidence and cause of false positive reaction about hepatitis C virus antibody detection in maintenance hemodialysis outpatients, in order to correctly deal with the antibody test reports and the doctor-patient communication problems. Methods: The hepatitis C virus antibody and RNA in 279 maintenance hemodialysis outpatients of General Hospital of Southern Theater Command of PLA were analyzed by cluster sampling, cross-sectional investigation and retrospective study from December 2019 to December 2022. Results: It was found that 81 patients were false positive for hepatitis C virus antibody,which accounted to 29.03%; S/CO values between 1.0 and 5.0 was 98.77%, and between 5.0 and 10.0 was 1.23%. All cases were reviewed by HCV-RNA three times,and were negative. Different detection methods and reporting procedures were chosen at the department of laboratory and the department of blood transfusion medicine,and the results were significantly different(P<0.01). Conclusion: The S/CO values of false positive reactions about hepatitis C virus antibody detection are mostly in the gray zone range and are related to the detection strategy and reporting procedure.

Uric acid is the final product of purine metabolism. It is an independent risk factor for a variety of cardiovascular and metabolic diseases, and even can cause changes in mood and cognitive function. In recent years, discussions about the effects of uric acid on the nervous system and cognitive function have received widespread attention. Many scholars have recognized the importance of uric acid, which is a naturally occurring antioxidant in the human body that can alleviate oxidative stress and thus play a neuroprotective role. However, other scholars believe that uric acid has pro-oxidative, pro-inflammatory and neurotoxic properties, which can cause damage to brain cognitive functions. Magnetic resonance imaging(MRI) technology can detect changes in the microscopic structure of the brain at an early stage and explore its relationship with cognitive function. This article will focus on the impact of uric acid on cognitive function and explore the application of MRI in this area.

Data mining (DM) is the process of extracting potentially valuable information and knowledge from large amounts of incomplete, noisy, fuzzy, and random real-world application data. This technology integrates artificial intelligence, databases, statistics and other multidisciplinary content, providing excellent convenience for the innovation and development of diseases management-related research. In recent years, the application of DM technology to diabetes management has achieved great results in the early prevention of diabetes, the prediction of diabetes complications and the prognosis of diabetes. However, the application of data mining technology in the field of diabetes is still in the exploratory stage in China, so this paper aims to make a detailed review of the classification of data mining technology and the application and progress of data mining technology in diabetes screening, diabetes complications prediction and improvement of the management quality of diabetes patients, so that medical staffs can have a clear and intuitive understanding of the application of data mining technology in diabetes management, and find the existing problems in the application of data mining technology in diabetes management. This paper will provide evidences for the fine management of healthcare professionals to facilitate the production of research results that are beneficial to patients and healthcare providers.