Objective: To develop a learning disabilities rating scale (LDRS) for school-aged children in China and test its reliability and validity. Methods: A primary school in Heyuan, Guangdong Province was randomly selected for Pretest and Formal Test, with sample sizes of 114 and 433, respectively. Pretest data were analyzed using reliability analysis, item analysis, and exploratory factor analysis (EFA). The formal test phase employed reliability analysis, confirmatory factor analysis (CFA), and criterion validity testing. Receiver operating characteristic (ROC) curve analysis was utilized to evaluate the screening capacity of LDRS. Results: The finalized LDRS comprises 5 dimensions with 29 items, categorized as reading comprehension, mathematics, written expression, attention, and emotional behavior. LDRS has been confirmed to possess excellent reliability, construct validity, and criterion validity. Using clinical diagnosis as the gold standard, ROC analysis determined an optimal cutoff score of 71.5 for LDRS, yielding a sensitivity of 0.906, specificity of 0.756, and a Youden index of 0.662. Conclusion: The LDRS is a dependable and effective instrument for screening individuals with learning disabilities, helping to assess learning disabilities among school-aged children in China.

This review summarized the research progress on the biomedical and biomechanical characteristics, influencing the speed ability of elite sprinters, aiming to provide a scientific and comprehensive reference for the assessment and selection of sprinters. The biomedical characteristics of sprinters' speed ability including the related genotyping, body morphology, and physiological functions and etc. The main researches showed: The ACTN3 gene has been confirmed to be closely associated with sprint and jump performance in sprinters; Muscle morphology and volume, as well as muscle fiber type, also have significant impacts on sprint performance; Physiological function monitoring studies have demonstrated that indicators, such as cardiopulmonary function and blood testosterone levels, can reflect athletes' physiological states in real-time and effectively assess training effectiveness. The importance of athletes' biomechanical characteristics in sprinting is also crucial. Research has confirmed that muscle length, maximum elongation of tendons and aponeuroses, and plantar flexor torque are equally key factors determining sprint performance. Furthermore, psychological factors, age, starting techniques, and the scientific training methods collectively influence athletes' performance outcomes.

Objective: To investigate the role and mechanism of cytoskeleton-associated protein 2-like (CKAP2L) in the proliferation, epithelial-mesenchymal transition (EMT), and migration of non-small cell lung cancer (NSCLC) cells. Methods: Potential NSCLC-related genes were screened using the GEO and TCGA databases. qRT-PCR, Western blotting, and immunohistochemistry were applied to detect CKAP2L expression in NSCLC tissues. The survival rate in NSCLC patients was analyzed using the Kaplan-Meier method. After treatment with CKAP2L shRNAs, CKAP2L overexpression plasmids, or Wnt/β-catenin inhibitor IWR-1, CKAP2L expression was measured by qRT-PCR and Western blot. Cell proliferation was assessed by CCK-8 and EdU staining. Cell migration was confirmed using a Transwell assay. Western blotting and immunofluorescence were used to monitor the expression levels of EMT-related and Wnt/β-catenin pathway proteins. Results: CKAP2L was upregulated in NSCLC tissues (P<0.001), and high CKAP2L expression was associated with poor prognosis in NSCLC patients. Compared with those in the overexpression control (OE-CTRL) group, the proliferation (P<0.01 or P<0.001), EMT and migration (P<0.01) of NSCLC cells were significantly enhanced in the CKAP2L-overexpressing (OE-CKAP2L) group; compared with those in the knockdown control (sh-CTRL) group, the proliferation (P<0.01 or P<0.001), EMT and migration (P<0.01) of NSCLC cells were significantly weakened in the CKAP2L-knockdown (sh-CKAP2L) group. CKAP2L upregulated Wnt/β-catenin pathway-related proteins (P<0.01). Conclusion: CKAP2L might accelerate NSCLC progression through the Wnt/β-catenin pathway, suggesting that CKAP2L may be a new target for treating NSCLC patients.

Bone homeostasis is a dynamic homeostasis process maintained by osteocytes, osteoblasts and osteoclasts, which is essential for the growth and development of bone. Protein phosphorylation and dephosphorylation can affect bone formation and development. Protein phosphatase 2A (PP2A), as an important member of the protein phosphatase family of cellular dephosphorylation, contains many subunits, has a complex structure and a wide range of functions. It can dephosphorylate proteins and participate in cellular biological processes like regulation of enzymes, transcription factors and signaling. It also plays a key role in the differentiation process of osteogenesis and osteoclasts. This review summarizes the regulatory role of PP2A in bone homeostasis and the research progress of its various inhibitors in the treatment of bone-related diseases, hoping to provide new ideas and theoretical basis for the drug treatment of bone-related diseases in clinical practice.

Objective: To investigate the protective effect of Lycium barbarum extracts (LBE, Ningxia, China) on the retinal structure and function in the mice administrated with optic nerve crush (ONC). Methods: The changes of flash electroretinogram (fERG) and retinal structure in the normal group, ONC-2 d group and ONC-7 d group were compared. Then, the effects of intraperitoneal injection of various dosages of LBE on the survival of retinal ganglion cells (RGCs) in the ONC-7 d group were compared. After that, the LBE dosage with the best protective effect was selected for the next experiment. Finally, we further studied the effects of 1.0 mg/kg LBE on the retinal ERG and structure preservation ONC-7 d group. In all the mentioned experiments, the full-field flash ERG was used to detect the changes in the a-wave and b-wave amplitude and latency under dark and light conditions in the living animals. The collected retinas were processed as the retinal flat whole-mounts and used for the immunofluorescent labeling of the RGCs, or as the frozen sections to be utilized for the fluorescent staining of the retinal bipolar cells, cones, synaptophysin (SYN), anti-postsynaptic density protein 95 (PSD95) and the reactive oxygen species (ROS). ImageJ software was used to count the numbers of the surviving RGCs, bipolar cells and cones, to calculate the percentage of the fluorescence-positive area (PP) of the bipolar cell protrusions in the inner plexiform layer (IPL), to measure the fluorescence intensity (FI) of the SYN in the IPL and outer plexiform layer (OPL), to measure the FI and PP of the PSD95 in the OPL, and to detect the red FI after the DHE detection. Results: Compared with the N and ONC-2 d groups, the numbers of the survival RGCs, bipolar cells and cones, the PP of the bipolar cell protrusion in the INL, and the FI of the SYN in the IPL and OPL decreased (P<0.05) in the ONC-7 d group. The FI of the PSD95 did not change (P>0.05); but the PP decreased in the OPL (P<0.050). The results of ERG examination showed that the amplitudes of a- and b-waves decreased under dark adaptation (P<0.01) and the reduction in the amplitude of b wave during light adaptation was significant 7 days after surgery (P<0.05). DHE staining showed that the ROS was increased 7 days after the operation (P<0.01). Compared with the control group, treatment with LBE could increase the survival of the RGCs (P<0.01) with the largest survival average density of RGCs in the 1.0 mg/kg LBE group (P<0.01). In addition, compared with the control group, 1.0 mg/kg LBE could increase the PP occupied by the bipolar cell protrusions in the IPL (P<0.05), the number of surviving cone cells (P<0.01), the FI of SYN in the IPL (P<0.01) and OPL (P<0.05), and the FI and the PP of PSD95 in the OPL (P<0.05). The improved function of the retina was shown by the increasing amplitudes under dark adaptation (P<0.05) and light adaptation (P<0.01). LBE reduced the FI of ROS (P<0.05). Conclusion: LBE has a protective effect on the retina after ONC injury in mice, and its protective effect may be related to its inhibition of oxidative stress in the retina.

Jintiange capsule is principally composed of bionic tiger-bone powder. Significant therapeutic effects have been demonstrated in areas including the enhancement of osteogenic activity, increase in bone density, inhibition of bone resorption, and reduction of bone loss, with positive therapeutic impacts observed on various clinical manifestations caused by osteoporosis. When combined with other medications for primary or secondary osteoporosis treatment, enhanced therapeutic efficacy has been documented. Bone mineral density and osteocalcin levels have been effectively improved through this combination therapy. The bone metabolism process has been regulated, patient pain has been significantly alleviated, and favorable safety profiles have been maintained. To evaluate the clinical value of Jintiange capsule combination therapy in osteoporosis treatment, relevant application studies are systematically reviewed in this article.

Objective: To analyze the chemical components of Artemisia argyi essential oil(AAEO) extracted by supercritical carbon dioxide combined with molecular distillation and evaluate its antibacterial, anti-inflammatory and tyrosinase inhibitory activities, and to discuss the application of AAEO in external preparation. Methods: AAEO was extracted by supercritical carbon dioxide combined with molecular distillation. The chemical components of AAEO were analyzed by gas chromatography-mass spectrometry (GC-MS). The inhibition zone diameter of AAEO against five test bacteria was tested by filter paper diffusion method, including Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested by broth microdilution method. The lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model was used to investigate the effect of AAEO on the mRNA expression levels of inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and inducible nitric oxide synthase (iNOS). The effect of AAEO on tyrosinase activity and melanin production was evaluated by human melanoma cell (A375 cell) model. Results: A total of 132 compounds were identified from AAEO, the main components of which were α-terpineol (7.08%), terpinen-4-ol (6.45%), β-caryophyllene (5.83%), eucalyptol (4.82%), endo-borneol (4.54%), 2-cyclohexen-1-ol (3.82%), d-camphor (3.09%), carveol (1.49%), and α-thujone (0.75%). AAEO showed significant antibacterial activity against the five tested bacteria, among which Candida albicans was highly sensitive. The MICs against Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans were 1.88, 3.75, 7.50, 15.00, 0.94 mg/mL, and the MBCs were 1.88, 7.50, 15.00, 30.00, 1.88 mg/mL respectively. AAEO could reduce the mRNA expression levels of IL-1β, IL-6, TNF-α, iNOS in RAW264.7 cells significantly (P<0.05). AAEO could inhibit tyrosinase activity in A375 cells significantly (P<0.05), and the production of melanin was reduced significantly (P<0.05). Conclusion: AAEO extracted by supercritical carbon dioxide combined with molecular distillation are rich in chemical components and has good antibacterial, anti-inflammatory and tyrosinase inhibitory activities. Its application value is worthy of in-depth study.

Objective: To investigate renal ischemia-reperfusion (I/R) injury inducing Parthanatos in renal tubular epithelial cells and the upstream signals that initiate this death pathway's activation. Methods: A mouse model of renal I/R injury and a hypoxia/reoxygenation injury model of renal tubular epithelial cells were established and pretreated with Poly ADP-ribose polymerase 1 (PARP-1) inhibitor 3-aminobenzamide (3AB) and antioxidant N-acetyl-L-cysteine (NAC). Hematoxylin and eosin (HE) staining was used to observe renal tissue pathological damage. Serum creatinine (Cre) and blood urea nitrogen (BUN) were detected to evaluate renal function. The cell counting kit-8 (CCK-8) and lactate dehydrogenase (LDH) assays were used to detect cell viability and mortality. 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) was used to detect intracellular reactive oxygen species (ROS). The activities of superoxide dismutase (SOD) and the levels of malondialdehyde (MDA) in cells were detected using corresponding kits. Enzyme-linked immunosorbent assay (ELISA) and Western blot were applied to detect DNA damage markers and the expression of Parthanatos-related proteins. Results: 3AB pretreatment alleviated I/R-caused renal tissue damage and renal dysfunction (P<0.05), and reduced hypoxia-reoxygenation-induced cell death (P<0.05). NAC pretreatment decreased the levels of intracellular ROS and MDA in hypoxia-reoxygenation cells (P<0.05), increased the activity of SOD (P<0.05), inhibited DNA damage and Parthanatos pathway activation (P<0.05), reduced cell death (P<0.05), and alleviated renal injury and renal dysfunction (P<0.05). Conclusion: I/R-caused oxidative stress can lead to DNA damage and activate PARP-1, which in turn promotes the occurrence of Parthanatos in renal tubular epithelial cells. This provides new ideas for the effective prevention and treatment of renal I/R injury.

Objective: To explore the relationship between dietary inflammation index and the risk of gynecologic malignant tumor. Methods: From January 2001 to March 2022,52 patients with gynecological malignant tumors were selected as case group, and 315 healthy persons were selected as control group. By filling in personal history questionnaire and food frequency questionnaire (FFQ25) to collect general demographic data and dietary intake, the dietary composition was calculated according to Chinese food composition table (2002 Edition), and personal DII values were ultimately calculated. Propensity score matching (PSM) was used to make the baseline data of the cohort similar, and Logistic regression was used to assess the association between DII values and the risk of gynecologic malignancy. To consult the medical examination reports of the two groups, inflammatory markers including neutrophil, lymphocyte, platelets, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and C-reactive protein (CPR) were analyzed. Results: By using multiple regression equation, the scheme of unadjusted confounding variables, scheme of adjusted Ⅰ and scheme of Adjusted Ⅱ showed that the DII value and the high DII score (0.62-4.82) were closely related to the occurrence of gynecological tumors, the difference was significant (P<0.01), and the results of sensitivity analysis were consistent with those before propensity score matching. The relationship between DII value and the occurrence of gynaecological tumors was found by smoothing curve fitting; through threshold analysis, it was found that the inflection point of DII value was 1, and the difference was statistically significant (P<0.01). The levels of inflammatory markers such as NLR, PLR and CPR in the case group were higher than those in the control group, the difference was statistically significant (P<0.01). According to threshold analysis, DII was divided into two groups, the levels of inflammatory markers in DII ≥1 group were significantly higher than those in DII<1 group (P<0.01). Conclusion: DII value has a curve relationship with the occurrence of gynecological tumors, and DII value can be used as a tool to predict the risk of gynecological tumors. When the DII value is higher than 1, the occurrence and development of gynaecological tumors need to be highly vigilant. The DII value can be used to guide the dietary structure of residents and carry out relevant nutrition education, so as to prevent the occurrence and development of gynecological tumors.

Autoimmune glial fibrillary acidic protein astrocytopathy (A-GFAP-A) is a rare autoimmune encephalitis discovered in the last decade. The diagnosis depends on clinical symptoms, imaging, and antibody testing. Most cases require several months or even longer to make a definite diagnosis. Due to early-stage diagnosis of this condition poses significant challenges, and there is a lack of relevant clinical guidelines, there are few reports at home and abroad on early diagnosis and treatment in such cases. This paper reported a case of high fever and headache as the initial symptoms, accompanied by persistent hyponatremia. With the help of ¹⁸F-fludeoxyglucose-positron emission tomography/magnetic resonance (¹⁸F-FDG PET/MR) imaging combined with antibody testing, the patient was diagnosed with anti-GFAP encephalitis approximately one month after symptom onset, and evaluated the patient's immunotherapy efficacy, leading to a favorable prognosis for the patient. This case provides a certain reference for the early clinical diagnosis and treatment of such patients.

Objective: The present study aimed to analyze the human papillomavirus (HPV) infection rate and subtype infection among 14 794 samples in Guangzhou, and to explore the association between HPV infection and ThinPrep Cytology Test (TCT). Furthermore, it evaluated the epidemiological features of HPV infection in Guangzhou, Guangdong Province, provided epidemiological insights into HPV infection among men, and assessed the significance of combined HPV and TCT testing in cervical cancer screening for women and in devising vaccination strategies against HPV. Methods: Sample data from 14,794 individuals who underwent genotyping for 37 HPV subtypes at the Second Affiliated Hospital of Guangzhou University of Chinese Medicine between January 2023 and December 2023 were collected. Concurrent analysis of TCT screening results was conducted to statistically examine the HPV infection characteristics across different ages, genders and seasons, as well as the relationship between single HPV infection, multiple HPV infection and TCT results. Results: The overall HPV infection rate stood at 29.42%. The most prevalent HPV subtype infections were HPV52 (21.55%), HPV58 (10.00%), HPV16 (9.17%), HPV51 (8.37%), HPV39 (7.56%), HPV61 (7.34%), and HPV53 (7.01%). The infection rate was 29.03% among women and 43.25% among men. The HPV infection rate exhibited a close correlation with age distribution, with statistically significant variations noted in the overall infection rate, single infection rate, and multiple mixed infection rate across different age groups ( X²=144.641, 30.797; P<0.001). Seasonal variations in infection rates were also observed, with rates of 28.03% in spring, 28.58% in summer, 29.56% in autumn, and 32.26% in winter. Among single infections, the highest infection rate of high-risk subtypes occurred in summer (43.53%), while among multiple infections, the higher infection rate was noted in autumn (35.02%). The TCT groups were predominantly characterized by single infections. Significant differences were discernible between single infections and multiple infections within each TCT group ( X²=90.497, P<0.001). Notably, TCT results also varied significantly among different age groups ( X²=32.871, P<0.001). Conclusion: The present study illuminated the epidemiological profile of HPV infection in the Guangzhou area, with an overall infection rate of 29.42%. HPV52, HPV58, and HPV16 emerged as the most frequent types. The overall HPV infection rate among men (43.25%) surpassed that among women (29.03%). The HPV infection rate showed a strong correlation with age distribution and was higher in summer and autumn compared to spring and winter. Additionally, this study identified significant differences in HPV infection rates among TCT groups and TCT results across different age groups, furnishing a scientific rationale for early HPV screening and presenting novel perspectives on cervical cancer prevention and treatment. In summary, this study contributes vital epidemiology data on HPV infection in Guangzhou, providing a scientific foundation for the formulation of targeted HPV prevention and control strategies and enhancement of cervical cancer diagnosis and treatment. It underscores the crucial role of male HPV infection in HPV prevention and control efforts.

Objective: To compare and analyze the anti-atopic dermatitis active components and mechanisms of action of Artemisia argyi essential oil extracted by water distillation and supercritical CO₂ fluid extraction based on network pharmacology and in vitro cell experiments. Methods: The essential oils of Artemisia argyi were extracted by water distillation and supercritical CO₂ fluid extraction, and their chemical components were determined by gas chromatography-mass spectrometry. Network pharmacology was used to predict the related targets and pathways of the two essential oils against atopic dermatitis, and the anti-atopic dermatitis activities of the two essential oils were explored in the lipopolysaccharides (LPS)-induced RAW264.7 cell inflammation model. Results: There were 65 compounds in Artemisia argyi essential oil obtained by steam distillation (AEOSD), 89 compounds in Artemisia argyi essential oil obtained by supercritical fluid extraction (AEOSFE), and 44 common compounds in the two essential oils. Network pharmacology analysis results found that eucalyptol and humulene were the main potential active ingredients of AEOSD against atopic dermatitis, eugenol, eucalyptol, and Copaene were the main potential active ingredients of AEOSFE against atopic dermatitis. AEOSD may affect the progression of atopic dermatitis by regulating the biosynthesis of nitric oxide (NO), the positive regulation of interleukin-6 (IL-6) production, and the NOD-like receptor signaling pathway, while AEOSFE may affect the progression of atopic dermatitis by regulating the biosynthesis of NO, T helper cell 17 (Th17) cell differentiation, PI3K-Akt signaling pathway, and inflammatory mediator regulation of TRP channels. In vitro experimental results showed that both AEOSD and AEOSFE could reduce the excessive release of NO in RAW264.7 cells induced by LPS, and reduce the expression of their target inflammatory factors IL-1β, IL-6, TNF and IL-1β, IL-6, PTGS2, respectively. Conclusion: AEOSD and AEOSFE differ in their chemical composition and target pathways against atopic dermatitis, but both have good anti-inflammatory activity on RAW264.7 cell.

Radiation retinopathy (RR) is primarily treated with intravitreal injections of anti-vascular endothelial growth factor (anti-VEGF), which is costly and requires repeated administration. Currently, there is no unified standard for the treatment of RR. This paper reports a case where anti-VEGF therapy was ineffective in Guangdong Provincial People's Hospital in 2018, but 577 nm subthreshold micropulse laser (SML) treatment successfully resolved the severe macular edema caused by radiotherapy for nasopharyngeal carcinoma, with stable therapeutic effects maintained over a 5-year follow-up period without recurrence. There have been no domestic reports on the use of SML treatment for RR macular edema. By reviewing the existing literature, SML is considered to be a cost-effective and non-invasive, allowing for repeatable procedures, it can provide a new strategy for the treatment of RR.

Objective: To isolate guaiane-type sesquiterpenes from leaves of Artemisia argyi H. Lév. & Vaniot, providing scientific evidence for the development and utilization of sesquiterpene compounds found in Artemisia argyi leaves. Methods: Compounds were isolated using conventional open-column chromatography and semi-preparative high-performance liquid chromatography. The structures of the compounds were elucidated by modern spectroscopic techniques. The cytotoxicity of the compounds was assessed by CCK-8 assay, and their inhibitory activity against nitric oxide (NO) production was evaluated by Griess reagent. Results: Ten guaiane-type sesquiterpenes were isolated and identified, comprising 2 unknown compounds (1-2) and eight known compounds (3-10). Compound 4 significantly inhibited NO production in LPS-stimulated RAW264.7 cells, with a half-maximal inhibitory concentration (IC₅₀) of (1.5±0.1) μmol/L. Conclusion: Notable anti-inflammatory activity has been identified in sesquiterpene compounds present in Artemisia argyi leaves. The identification of these novel sesquiterpenes enhances the chemical profile of Artemisia argyi leaves and offers strong scientific support for their potential applications in the pharmaceutical industry.

Currently, there is limited systematic research on postoperative rehabilitation protocols for Maisonneuve fractures regarding functional recovery and long-term athletic performance maintenance in elite athletes. This article presented a case report on personalized and sport-specific rehabilitation protocol after Maisonneuve fracture surgery of professional basketball player, integrating progressive weight-bearing training, isokinetic strength enhancement, and neuromuscular control exercises. By 32 weeks postoperatively, the patient's ankle ROM and muscle strength had recovered to over 90% of the contralateral side, meanwhile the visual analogue scale (VAS) pain score had decreased from 7 to 1. Through a 10-year follow-up, the athlete had maintained professional competitive performance, with the American orthopaedic foot & ankle society (AOFAS) score consistently at 100 points, while without any complications such as post-traumatic osteoarthritis observed. This case illustrated that a staged rehabilitation protocol based on sport biomechanical characteristics could effectively promote functional reconstruction in athletes. Furthermore, the long-term safety and athletic-performance outcomes observed in this protocol might provide valuable insights for similar clinical cases.

Obesity is a global epidemic and the proportion of obese and overweight people is increasing. Obesity is a risk factor for a variety of non-infectious diseases involving multiple organs throughout the body, including the respiratory system. The effects of obesity on lung function are characterized by mechanical compression of the lungs and low-grade inflammatory effects on the bronchi. Mechanical compression is mainly manifested by decreased respiratory compliance, altered lung volumes, increased airway resistance, altered regional lung ventilation and lung ventilation/perfusion, and respiratory muscle overload. A low-grade inflammatory state can cause inflammatory changes in the bronchial wall and induce airway remodelling. Body mass index (BMI) is a simple and convenient way to categorise the degree of obesity, but does not account for patterns of regional fat distribution. Different patterns of fat distribution have different effects on lung function. Lung function tests can non-invasively and indirectly show changes in lung function in obese people, but they reflect changes in the respiratory system as a whole and cannot directly and dynamically show changes in lung function. CT and MRI imaging tests, can not only accurately calculate the fat content and distribution, but also quantitatively analyse the changes in the lung structure, and more importantly, can also observe the dynamic changes in the thorax, lungs, and respiratory muscles under free breathing, so as to detect the changes in the lung function as early as possible, and thus carry out weight loss interventions in time. This paper will discuss the pathophysiological mechanisms of obesity-induced lung changes; the progress of obesity-related chest imaging and future research hotspots.

Objective: To investigate the changes in systemic immune inflammatory index (SII), D-dimer level and SOFA score in patients with sepsis and their prognostic value assessment. Methods: Retrospectivly analyzed 114 patients with sepsis hospitalized in the Second Affiliated Hospital of Xinjiang Medical University during the period from July 2021 to July 2023. 43 patients who underwent health checkups during the same period were selected as the control group. Laboratory tests such as coagulation series and blood specimens were performed after admission to the hospital to derive SOFA score. All sepsis patients were followed up for 28 d. Based on the clinical outcomes during hospitalization, the patients were divided into 37 cases in the survival group and 77 cases in the death group. The differences between SII, D-dimer level and SOFA score in the three groups were compared. Univariate and multivariate Logistic regression were used to analyze the baseline data of sepsis patients, to derive the independent factors influencing the death of sepsis patients, and to draw forest plots; Spearman correlation analysis of SII index, D-dimer level and SOFA score with prognosis of patients with sepsis; survival curves were plotted by ROC curves assessing SII, D-dimer level, SOFA scores and the combination of all three. Finally, calibration curves and clinical decision curves were plotted based on the R after the model was re-sampled 500 times using the Bootstrap method. Results: Patients in the sepsis death group had significantly higher SII, D-dimer, and SOFA score than those in the survival and control groups, and the differences were statistically significant. The case fatality rate of patients with sepsis was 68%. Analysis of one-way Logistic regression showed that SII, D-dimer level, and SOFA score were correlates of death in sepsis patients. Multifactorial Logistic regression analysis showed that high SII, high D-dimer level, and high SOFA score were independent risk factors for death in patients with sepsis. SII, D-dimer level, and SOFA score were positively correlated with death in patients with sepsis. The results of the ROC curve analysis showed that the area under the curve of SII, D-dimer level, SOFA score, and the combined test to predict death in patients with sepsis, were 0.822, 0.888, 0.874, 0.967 respectively. The diagnostic sensitivity was 73.700% and specificity was 75.000% at the optimal cut-off value of 2 063.5 for SII. The diagnostic sensitivity was 85.500% and specificity 80.600% at the optimal cut-off value of 3.8 mg/mL for D-dimer level. The diagnostic sensitivity was 75.500% and specificity was 86.100% at the cut-off value of 9.500 points for SOFA score. The sensitivity of the combined test was 93.400% and the specificity was 91.700%. Conclusion: Elevated levels of SII, D-dimer and SOFA score are independent risk factors for death in sepsis patients, the constructed prediction model has high goodness of fit and clinical applicability, and the combination of the three tests has higher predictive value for sepsis patients.

Objective: To investigate the efficacy and safety of different stimulus dosage of modified electroconvulsive therapy (MECT) in the treatment of depressive episode. Methods: Sixty-five patients with depressive episode according to Diagnostic and Statistical Manual of Mental Disorders, 4th edition (DSM-IV) were randomly divided into treatment group (32 patients) and control group (33 patients). The patients in the treatment group received low stimulus dosage of MECT while the patients in the control group were treated with routine stimulus dosage of MECT. The treatment session was 6 times in both groups. The stimulus electrodes were placed on bifrontal. Hamilton depression scale-17 item (HAMD-17) and MATRICS consensus cognitive battery (MCCB) were used to evaluate the clinical symptoms and cognitive function at baseline, after 3 times of MECT, and after 6 times of MECT. Heart rate, systolic blood pressure, and diastolic blood pressure were recorded before and after stimulus in the first session of MECT treatment. Adverse reactions related to MECT were also recorded. Results: Fifty-six (29 patients in treatment group and 27 patients in control group) completed MECT treatment. The improvement rate of treatment group and control group was 68.97% and 70.37%, the remission rate was 51.72% and 62.96%, respectively. There was significant decrease of HAMD-17 score after MECT compared with baseline in both groups while there was no significant difference between groups. Compared with baseline, the heart rate, systolic pressure, and diastolic pressure increased significantly after stimulus in the first MECT session, while there was no significant difference between groups. There was no significant difference of MCCB score after MECT treatment compared with baseline and between groups. One patient experienced delirium in the treatment group, while in the control group, three patients experienced MECT related side effects involving headache, nausea and vomiting, muscle pain, and delirium. Conclusion: The efficacy of low stimulus dosage MECT treatment was equivalent to the conventional stimulus dosage in treating depressive episodes while with fewer adverse reactions.

Essential oil is the main pharmacodynamic substance of Artemisia argyi. Thus, the quality control of Artemisia argyi essential oil is crucial for its stable therapeutic effect. At present, the extraction methods of Artemisia argyi essential oil include steam distillation, supercritical CO₂ extraction, organic solvent extraction, ultrasonic-assisted extraction, etc. This review summarized quality control and extraction technology of Artemisia argyi essential oil in recent years, in order to provide scientific basis for the comprehensive development and application of Artemisia argyi.

Objective: To investigate the clinical characteristics of fibromyalgia syndrome (FMS) complicated by migraine. Methods: The study included 210 FMS patients who were treated at the Third Affiliated Hospital of Qiqihar Medical University from January 2021 to December 2023. General and clinical data were collected. Assessment was conducted using scales such as the wide spread pain index (WPI), symptom severity scale (SSS), fibromyalgia severity (FS), pain score, anxiety score, sleep score, and somatic symptom score. Results: Among the 210 FMS patients, 108 (51.43%) had concomitant migraine. Compared with FMS patients without migraine, those with migraine had significantly higher scores in FMS duration (P=0.001), WPI score (P=0.003), SSS score (P=0.001), FS score (P=0.002), pain score (P=0.008), anxiety score (P=0.005), sleep score (P=0.004), and somatization symptom score (P=0.009). In FMS patients with migraine, FS scores were positively correlated with headache duration (P=0.013), headache frequency (P=0.019), headache duration (P=0.037), FMS duration (P=0.025), pain score (P=0.028), anxiety score (P=0.030), sleep score (P=0.035), and somatization symptom score (P=0.008). Conclusion: The presence of migraine exacerbates the clinical symptoms of FMS, and there is a correlation between FMS symptoms and headache symptoms, anxiety, and sleep disturbances when FMS is complicated by migraine.

Objective:To investigate the current status of oral health knowledge, attitude and practice (KAP) among elderly individuals aged 60 years and above in a community in Nanning City, and to explore the factors influencing these, in order to provide a reference for the development of oral health management programs for the elderly. Methods:A cross-sectional study was conducted using convenience sampling to select 488 elderly individuals from two communities in Qingxiu District, Nanning City, based on inclusion and exclusion criteria. A questionnaire survey was administered, and SPSS 26.0 was used to perform descriptive analysis on general information and the oral health KAP of the elderly, utilizing frequency, percentage, mean, and standard deviation. Two independent sample t-tests, one-way analysis of variance, and multiple stepwise regression analysis were used to explore the factors influencing oral health KAP among the elderly. Results:The scores for oral health knowledge, attitude, and practice among the elderly were (10.00±4.39), (8.05±3.64), and (22.31±7.58), respectively. Single-factor analysis showed statistically significant differences in oral health KAP scores based on age, education level, and personal monthly income (P<0.05). Multiple stepwise regression analysis revealed that higher education level, younger age, and higher monthly income were associated with better oral health KAP scores. Additionally, elderly individuals with a spouse had significantly higher attitude scores compared to those without a spouse. Conclusion:The oral health knowledge, attitude, and practice of elderly individuals in this community in Nanning City are at a relatively low level and are significantly influenced by factors such as age, education level, and personal income.

Objective: To construct a strong inducible expression system based on T7-like RNA polymerase MmP1 in Escherichia coli Nissle 1917 (EcN) to achieve efficient expression of the target protein. Methods: Constructing different inducible expression systems in EcN for “dose-response” characterization to screen out the best-performing induced system with low leakage and high induction expression level. Optimizing the expression output and dynamic range of the T7-like system by integrating the T7-like RNA polymerase (MmP1) expression on the chromosome of EcN, namely strain LM01, thereby constructing promoter mutation library of P_MmP1 for tunable induced expression output. T7-like induced system based on MmP1 was used to control the expression of superoxide dismutase (SOD) of high yield, giving strong evidence for protein expression of T7-like system in EcN. Results: Several inducible systems were successfully constructed and characterized in EcN. Of which, the T7-like induction system based on MmP1 RNA polymerase displayed the best-performing saturated induction output with maximum dynamic range reaching up to 909-fold. After the MmP1 polymerase expression module was integrated into the genome of EcN, the saturated induction level was increased by 66.8%. The construction of P_MmP1 promoter library provides a wide range of inducible expression output (dynamic range), spanning 65 to 1 097 fold. The T7-like (MmP1) system demonstrated the highest soluble SOD protein production yield of 435.7 mg/L, which was 4.02-fold higher than vanillic acid induction system. In addition, the maximum enzymatic activity of SOD achieves 391.3 U/mL. Conclusion: T7-like (MmP1) induction system can be successfully constructed in EcN with high saturated expression level, low basal leakiness, as well as controllable dynamic range of tunable gene expression control for efficient protein synthesis in the coming future.

Objective: To explore the distribution pattern and related influencing factors of dampness syndrome in patients with precancerous lesions of gastric cancer (PLGC) through epidemiological investigation. Methods: Epidemiological data of PLGC patients who visited Guangdong Provincial Hospital of Chinese Medicine from June 2023 to November 2023 were collected. After grouping them into different dampness syndrome types, analysed the effects of social demographics data, lifestyle, and disease conditions on PLGC patients with different dampness syndrome types. Finally, the influencing factors with statistical difference were analysed by unordered multiclassified Logistic regression analysis. Results: A total of 197 PLGC patients were collected in this study, including 35 patients with non-dampness syndrome, 30 patients with dampness-heat syndrome, 87 patients with spleen-dampness syndrome, 17 patients with wind-dampness syndrome, and 28 patients with at least 2 different types of dampness syndrome. Correlation analysis of non-dampness syndrome group, dampness-heat syndrome group, spleen-dampness syndrome group, and wind-dampness syndrome group revealed that there were statistical differences among the four groups in depression, dietary habits, food intake, smoking history, and staying up late. Logistic regression analysis of the above factors revealed that depression and having smoking history were independent influencing factors of dampness-heat syndrome, and staying up late was an independent influencing factor of spleen-dampness syndrome. Conclusion: Dampness syndrome was widely present in PLGC patients, and depression and a history of smoking may independently affect dampness-heat syndrome in PLGC, while staying up late may independently affect spleen-dampness syndrome in PLGC.

Rapid advancements of 3D printing technology in the biomedical field have provided new solutions for vascular regeneration. This paper summarized applications of 3D printing in vascular regeneration, highlighting innovations in biomaterial design, vascular structure construction and cell carrier development. It details the characteristics of common vascular scaffold materials, the release systems of bioactive factors, and the establishment of simulation models. By analyzing the impacts of printing technology, biomaterials and active factors on vascular regeneration in practical applications, this paper discussed how to further utilize 3D printing technology to accurately build biocompatible vascular tissue models. Additionally, it addressed future directions including the selection of biocompatible materials, printing precision, cell survival rate, and maintenance of function. 3D printing technology has broad prospect in the field of vascular regeneration, but challenges in biocompatibility and functionality must be overcome for broad clinical application.

Objective: To screen the compounds with high inhibitory potency on influenza virus from Traditional Chinese Medicine (TCM) extracts. Methods: Neuraminidase (NA) inhibitory activity assay was used to screen 148 TCM extracts, among which Curcuma longa L. extract displayed the best NA inhibitory activity. The bioactive compounds were isolated from the extract of Curcuma longa L. using at-line nanofractionation(ANF). The structures of these compounds were analyzed using mass spectrometry and their interactions toward NA were examined through molecular docking. Results: NA inhibitory rate of the extract from Curcuma longa L. was found to be 92%. A total of eight bioactive compounds, mainly curcumin analogues, were identified from Curcuma longa L. extract, indicating curcuminoids play a significant role for NA inhibition. Curcuminoids have a different mechanism of action compared to oseltamivir. Conclusion: ANF technology can play an important role in the screening of neuraminidase inhibitors. This finding not only provides a scientific basis for the potential clinical use of Curcuma longa L. against the influenza virus but also establishes a foundation for the development of novel influenza antivirus drugs.

Objective: To elucidate the effect of targeting acidic nucleoplasmic DNA-binding protein 1 (And-1) on the sensitivity of ovarian cancer cells to the PARP inhibitor niraparib and explore its potential underlying mechanism. Methods: First, And-1 expression were analyzed in ovarian cancer tissues using data from the GEO and TCGA databases, and its impact on patient survival outcomes were examined. Next, ovarian cancer cell lines with stable And-1 knockdown were generated via shRNA technology, and cell proliferation was assessed using growth curves. Cellular sensitivity to niraparib was then measured with CCK-8 assay and further validated through colony formation assays. Additionally, Western blot was performed to assess the expression of the apoptosis-related protein cleaved PARP1 following niraparib treatment, and flow cytometry was used to assess apoptosis rates. Results: And-1 expression was significantly elevated in ovarian cancer tissues compared to normal tissues, and higher And-1 levels were significantly associated with worse prognosis (HR>1,P<0.05). In cell-based experiments, And-1 depletion in ovarian cancer cell lines A2780 and OVCAR3 led to a significant reduction in cell proliferation (P<0.05). Moreover, And-1 depletion markedly lowered the IC₅₀ value of niraparib (P<0.05), and colony formation assays confirmed increased sensitivity to niraparib (P<0.05). Additionally, Western blot analysis showed a marked upregulation of cleaved PARP1 (P<0.05), while flow cytometry revealed a substantial increase in apoptosis (P<0.05). Conclusion: Targeting And-1 enhanced the sensitivity of ovarian cancer cells to niraparib, likely through the promotion of apoptosis.

Hepatocellular carcinoma (HCC) accounts for 75%~85% of primary liver cancers. Due to its rapid progression, high invasiveness, and lack of effective therapeutic targets, HCC has an extremely poor prognosis. Pyruvate kinase M2 (PKM2), a key rate-limiting enzyme in glycolysis, not only sustains the malignant phenotype of HCC cells by enhancing aerobic glycolysis but also promotes HCC progression through non-glycolytic pathways, such as inducing an immunosuppressive tumor microenvironment. Currently, various PKM2-targeting agents (including natural products, nucleotide-based drugs, etc.) have demonstrated promising anti-HCC effects in both in vitro and in vivo studies. Although PKM2 presents excellent translational value as a therapeutic target for HCC in preclinical research, no PKM2-related clinical trials for HCC have been initiated to date. Worldwide, only 10 clinical trials directly related to PKM2 exist, most of which are either recruiting or terminated. This status is incongruent with the significant therapeutic potential demonstrated for PKM2 in basic research. This review systematically summarizes the latest research progress on PKM2 in HCC, preliminarily explores the potential reasons for the discrepancy between basic and clinical findings regarding PKM2 in HCC, and aims to provide new research perspectives for the targeted intervention in HCC.

Objective: To explore the relationship between triglyceride-glucose index (TyG) and all-cause mortality in patients with acute myocardial infarction (AMI). Methods: The data for this study came from the ICU Information Mart for Intensive Care (MIMIC) database. The clinical outcome is hospitalized mortality. The relationship between TyG index and hospitalized mortality was analyzed using restricted cubic spline (RCS) model, and the Cox proportional hazards model was used to evaluate the association between different TyG groups and in-hospital all-cause mortality. Results: A total of 501 patients were included in the study, including 170 in the low TyG group (TyG<8.80), 167 in the medium TyG group (8.80≤TyG<9.35), and 164 in the high TyG group (TyG≥9.35). With the middle TyG group as the reference group, in univariate analysis, the hazard ratio (HR) of the low TyG group was 1.84(95%CI: 0.68~4.98, P=0.229), the HR of the high TyG group was 3.02(95%CI: 1.19~7.65, P=0.02); in Model Ⅰ with adjusted variables, the HR of the low TyG group was 1.91(95%CI: 0.70~5.23, P=0.209), the HR of the high TyG group was 3.68 (95%CI: 1.44~9.42, P=0.007); in further adjusted Model Ⅱ, the HR of the low TyG group was 4.47(95%CI: 2.17~9.21, P<0.001), the HR of the high TyG group was 2.00(95%CI: 1.01~3.94, P=0.045). Conclusion: The research results indicate that TyG index, as a simple and easily accessible laboratory parameter, has important value in evaluating the prognosis of patients with acute myocardial infarction. A higher TyG index is significantly correlated with an increase in in-hospital mortality.

Objective: To evaluate the effects of simplified finger exercise on improvement of comfort degree and decrease related complications during transradial coronary procedures. Methods: This study randomized 302 patients who underwent a transradial coronary procedure in Huangshi central hospital during 2022 to one of the following 2 groups, (i) simplified finger exercise in addition to routine pre-procedure care (test group, 151 patients), (ii) routine pre-procedure care (control group, 151 patients). The achievement ratio of radial puncture, incidence of radial artery spasm and dissection (RAS, RAD), change of limb circumference, and painfulness of the limb were observed and analyzed between two groups. Results: Compared to the control group, test group had a higher achievement ratio of radial puncture, lower incidence of RAS and RAD, slighter tumidness of limb and patient felt less pain in test group. Conclusion: Simplified finger exercise has a positive effect on improvement of a patient's comfort degree and decrease related complications during transradial coronary procedures.

Objective: To evaluate the feasibility and the superiority of AI-assisted compressed sensing (ACS) in knee joint MRI. Methods: Thirty-six participants who underwent both the conventional scanning and ACS scanning of knee joint MRI in Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology during the period of 2021 to 2022 were included in this study. The acquisition time of each scanning sequence, selected the bone marrow, muscle, anterior cruciate ligament and posterior cruciate ligament as regions of interest on coronal PD images, and calculated the signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) of these region of interst (ROI) were recorded. We evaluated the subjective image quality, and compared the objective image quality. The Wilcoxon signed-rank test was used to compare the objective image quality, and the kappa coefficient was used to statistically analyze the subjective scores. Results: Compared with conventional group, the total acquisition time of knee joint MRI in ACS group was shortened from 461 s to 245 s. There was no significant difference in SNR and CNR of bone marrow, anterior cruciate ligament and posterior cruciate ligament between the two groups (P>0.05). And there was no significant difference in the subjective scores of the two groups (P>0.05). In addition, the subjective measurement result of the two radiologists was in good agreement (Kappa_acs=0.79,Kappa_control=0.72). Conclusion: AI-assisted compressed sensing can significantly shorten the acquisition time without influencing the image quality, which has great potential in knee joint MRI. This technology can provide new ideas for rapid magnetic resonance imaging.

Objective: To explore the curative effect of electrostatically spun astragalus polysaccharide composite nanofibre dressings on lower limb venous ulcers patients. Methods: From April 2023 to December 2023, 92 patients with lower limb venous ulcers admitted to a tertiary hospital in Sichuan Province were selected and divided into experimental group and control group according to the random number table method in a ratio of 1∶1, with 46 cases in each group. The control group used conventional dressing changes, and the experimental group added electrostatically spun astragaloside composite nano-fibre dressing on the basis of the conventional dressing changes, and the intervention lasted for 4 weeks. The healing rate, number of dressing changes and quality of life scores of patients in both groups before and after 4 weeks of intervention were recorded, and the healing time was assessed after 3 months of follow-up. Results: After 4 weeks of intervention, the healing time, number of dressing changes and quality of life scores of patients in the experimental group were lower than those of the control group, and the difference was statistically significant (P<0.05). The time effect of healing rate, the interaction effect between groups and time, and the between-group effect were statistically significant (P<0.05). The healing rate of patients in the test group was higher than that of the control group after the 1st week of intervention, after the 2nd week, after the 3rd week, and after the 4th week (P<0.05). Conclusion: Electrostatically spun astragalus polysaccharide composite nanofibre dressing can improve the healing rate of patients with lower limb venous ulcers, reduce the number of dressing changes, shorten the healing time, and improve the quality of life of patients.

Objective: Network pharmacology, molecular docking and animal experiments were used to explore the mechanism of action of Wenyang Jiedu Granules on acute lung injury associated with influenza A virus infection. Methods: The active ingredients of Wenyang Jiedu Granules were collected based on the herbal medicine group review (HERB) database, and their ingredient targets were predicted using the SwissTargetPrediction database. Influenza A virus infection disease targets were retrieved using the GeneCards, DisGennet, Pharmgkb, and CTD databases to locate intersection targets. The STRING platform was used to draw the PPI network diagram, and the CytoScape 3.9.1 software was used to construct the TCM-ingredient-target network, and gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) analysis were performed. The AutoDockTools1.5.7 software was used for molecular docking to explore the potential mechanism, and the influenza A virus-infected mouse model was used for drug effectiveness and mechanism verification. Results: The core active ingredients of Wenyang Jiedu Granules in the treatment of acute lung injury are Tangeretin, Magnococline, Sexangularetin, Retusin, Nomilin, etc, and the core targets are JUN and TP53., SRC, STAT3, EGFR and PIK3CA, etc. GO analysis mainly involved biological processes such as phosphorylation, cytokine-mediated signaling pathways, while KEGG analysis mainly involves signaling pathways such as phosphatidylinositol-3-kinase (PI3K)-AKT signaling pathway. Molecular docking showed that the key components had good binding activity with the core targets. The animal experimental results of this study showed that Wenyang Jiedu Granules can alleviate the pathological damage of mouse lung tissue caused by influenza A virus infection and reduce the expression of inflammatory factors(IL-6, IL-1β, TNF-α, IFN-γ) in lung tissue. Western blot results showed that Wenyang Jiedu Granules could inhibit the expression of PI3K/AKT/NF-κB signaling pathway related proteins. Conclusion: Wenyang Jiedu Granules may inhibit PI3K/AKT/NF-κB signaling pathway through ingredients such as nocturine and nomiline in the treatment of influenza A virus infection-related acute lung injury.

Objective: Based on Donabedian “structure-process-outcome” (SPO) three-dimensional quality evaluation model, this study adopts Delphi method and analytic hierarchy process construct the quality evaluation index system of nosocomial infection management in general hospitals. Methods: Through literature review and group discussion, the indexes of nosocomial infection management quality evaluation were initially drawn up, and 20 experts were selected by Delphi method for two rounds of correspondence consultation to determine the index system of nosocomial infection management quality evaluation, and the weights of each index were calculated by analytic hierarchy process. Results: The positive coefficient of the two rounds of expert correspondence was 100%. The expert authority coefficients were 0.869 and 0.877, Kendall's W were 0.156 and 0.219, respectively, and finally formed a 3-dimensional, 21-item nosocomial infection management quality evaluation index system. Conclusion: After two rounds of expert consultation, the indexes were modified and sorted out, and the results of the selected quality evaluation indexes of nosocomial infection management were reliable and the weights of each index were reasonable, which could provide an objective and quantifiable basis for nosocomial infection management.

Objective: To analyze the relationship among the RNA expression levels of interferon-induced transmembrane protein 3 (IFITM3) and Toll-like receptor 4 (TLR4) in peripheral blood mononuclear cells and T helper 17 (Th17) cells/regulatory T cells (Tregs) balance and disease severity in children with respiratory syncytial virus (RSV) bronchiolitis. Methods: A total of 108 children with RSV bronchiolitis were selected as the RSV infection group, and divided into the mild group (n=45), the moderate group (n=40) and the severe group (n=23) according to the severity. At the same time, 105 healthy children were selected as the normal control group. The mRNA expression levels of IFITM3 and TLR4 mRNA expression level, Th17 cell count, Tregs cell count and Th17/Tregs ratio in peripheral blood were compared between the RSV infection group and the normal control group. Pearson correlation was used to analyze the relationship between above-mentioned indicators and Th17/Tregs balance. Spearman correlation was used to analyze the relationship between above-mentioned indicators and disease severity. The value of above indicators in evaluating disease severity was analyzed. Results: The mRNA expression levels of IFITM3 and TLR4, Th17 cell count and Th17/Tregs ratio in peripheral blood of the RSV infection group were significantly higher, and the level of Tregs in the RSV infection group was lower compared to the normal control group (P<0.05). Pearson correlation analysis revealed that the mRNA expression of IFITM3 and TLR4 was positively correlated with Th17/Tregs ratio (P<0.05). The mRNA expression levels of IFITM3 and TLR4 and Th17/Tregs ratio in peripheral blood increased in order from the mild group (P<0.001), the moderate group to the severe group (P<0.001). The mRNA expression levels of IFITM3 and TLR4 were positively correlated with disease severity (r=0.505, 0.517, P<0.05). Receptor operation characteristic (ROC) curve analysis showed that the area under curve (AUC) and sensitivity of combined evaluation with the three were 0.927 and 91.3%, higher than those of single prediction (P<0.05). Conclusion: The mRNA expression levels of IFITM3 and TLR4 in peripheral blood of children with RSV infection induced bronchiolitis are significantly elevated, and there exists Th17/Tregs imbalance. The mRNA expression levels of IFITM3 and TLR4 in peripheral blood can reflect Th17/Tregs balance and the severity of the disease to some extent.

Objective: Preliminary construction of a comprehensive scoring system based on qualitative indicators of ^99mTc-SC lymphoscintigraphy to explore the predictive value of ^99mTc-SC lymphoscintigraphy in assessing the efficacy of comprehensive decongestive therapy for secondary lower limb lymphedema following gynecological cancer surgery. Methods: A retrospective analysis was conducted on the data of 48 gynecological cancer patients at the First Affiliated Hospital of Sun Yat-sen University from January 2018 to March 2023 who developed secondary lymphedema following surgical procedures and received comprehensive decongestive therapy. Subsequently, the patients were stratified into effective (n=28) and ineffective treatment (n=20) groups based on predefined research criteria, with both clinical information and ^99mTc-SC lymphography data collected. The qualitative indicators of lymphography primarily encompassed: ① visibility of main lymphatic vessels' uptake pattern; ② identification of obstruction site in the pelvis or knee joint; ③ classification of subcutaneous soft tissue drainage type in lower limbs; ④ assessment of collateral lymphatic circulation presence. Results: There was a statistical difference in the delayed phase of lower limb lymphatic drainage and comprehensive score between the two groups (P<0.05). The predictive performance of the comprehensive score is significantly superior to that of the single qualitative indicator of lymphoscintigraphy. The sensitivity, specificity, and accuracy of prognostic assessment based on the comprehensive scoring system derived from nuclear lymphatic imaging were 96.4%, 85.0%, and 91.7%, respectively. Conclusion: Preoperative lymphoscintigraphy may be valuable for predicting the response to treatment of gynecologic cancer-related lymphedema following comprehensive decongestive therapy. The late-stage soft tissue bypass classification and comprehensive score serves as the primary independent predictor, and a scoring system that integrates multiple qualitative features can significantly enhance the sensitivity and accuracy of prognosis prediction.

Objective: To explore the potential targets and mechanism of procyanidin B2 (PB2) against prostate cancer (Pca) based on network pharmacology. Methods: The core targets of PB2 against Pca were predicted by network pharmacology, and the biological processes and key signaling pathways were enriched and analyzed. CCK-8 method was used to detect the anti-Pca activity of PB2 in vitro. The anti-Pca effect of PB2 was characterized by colony formation and scratch test. The core targets and key signaling pathways predicted by network pharmacology were verified by RT-qPCR and Western blot. Results: A total of 30 intersection targets of PB2 and Pca were screened based on network pharmacology. Among them, janus kinase 2 (JAK2), albumin (ALB), matrix metalloproteinase 9 (MMP9) and phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) were the key targets of PB2 in the treatment of Pca. MAPK-JAK2/STAT3 is the key pathway. The results of in vitro experiments showed that PB2 could significantly inhibit the proliferation, colony formation and migration of human prostate cancer cells (LNCAP cells). PB2 also significantly down-regulated the mRNA expression levels of core targets ALB, MMP9, PIK3R1 and JAK2 and inhibited the activation of MAPK-JAK2/STAT3 pathway. Conclusion: PB2 may treat prostate cancer through multi-target and multi-pathway synergy; pB2 can effectively inhibit the proliferation and migration of LNCAP cells, down-regulate the mRNA expression of core targets and inhibit the activation of key signaling pathways, thereby exerting an anti-Pca effect.

Objective: To characterize the blood-borne occupational exposures among healthcare workers in a tertiary general hospital in Shenzhen from 2019 to 2023 and determine the influencing factors, aiming to provide insights for continuously improving the management of occupational exposures and strengthening the protective measures. Methods: An observational descriptive study was performed by a retrospective retrieval of five-year data of exposures to blood among healthcare workers in a tertiary general hospital in Shenzhen. The demographic distribution, exposure routes and exposure sources were descriptively statistically analyzed. Results: From 2019 to 2023, a total of 359 cases of blood-borne occupational exposures were reported. The annual incidence rate fluctuated, of which the highest was 3.59% in 2019 and the lowest was 1.53% in 2022. The exposure mainly occurred in the second half of the year. The majority of the affected individuals were female and nurses, with 79.39% of them having less than 5 years of work experience. 84.68% of occupational exposures were caused by sharp instrument injuries, mainly occurring during needle withdrawal and disposal (22.70%) and surgery (21.05%). The main exposure sites included ward (42.34%) and operation rooms (20.61%). Most of exposure sources were tested negative for blood-borne pathogens except for 131 cases. The top three pathogens were hepatitis B virus, Treponema pallidum and hepatitis C virus. Follow-up monitoring suggested that 77.78% of the exposed individuals completed the recommended preventive medication and no post-exposure infection occurred after exposure. Conclusion: The blood-borne occupational exposure reported in this hospital during 2019 and 2023 was mainly needle stick injuries in junior nurses, and the main pathogen exposed was hepatitis B virus. No post-exposure infection occurred. Nurses are high-risk group of occupational exposure, and the prevention of needle-stick injury calls for our great concern. Meanwhile, vaccination against pathogens from occupational exposure especially hepatitis B, should be paid great attention as an effective protective measure.

Objective: The purpose of this study was to monitor and analyze the sleep rhythm characteristics and their consequential impacts on training performance, to provide scientific sleep guidance for athletes and improve training efficiency. Methods: Sleep and training data of 23 athletes from the Jiangsu Provincial Fencing Team from April to September 2023 were collected by HUAWEI band 6. We used night sleep duration, the adjusted mid-point of sleep on free days (MSFSc) and social jet lag (SJL) to assess the objective sleep characteristics of athletes. The classification of chronotypes was determined by the pivotal metric MSFSc, categorizing individuals into early type (<3:00 AM), intermediate type (3:00-5:00 AM), and late type (>5:00 AM). SJL is a measure of circadian rhythm disruption. We used the mean heart rate, maximum heart rate and training impulse (TRIMP) during training to evaluate the athlete's training. Results: 2 459 days of sleep data and 1 798 days of training data were collected. In 2 459 days of sleep monitoring, 57.2% of the days showed less than 8 hours of night sleep duration. The MSFSc of the athletes was (4:52±1:30), with approximately 60.87% categorized as intermediate types and 39.13% as late types; no early types were identified. SJL was (1.50±1.08) hours, with 65.22% of athletes experiencing more than 1 hour of SJL. SJL was strongly correlated with MSFSc(r=0.845,P<0.001). The mean heart rate, maximum heart rate and TRIMP of late types during training were lower than intermediate types; The mean heart rate and maximum heart rate of the athletes with higher SJL were also lower than those with lower SJL. Conclusion: Athletes generally experience sleep deprivation and circadian rhythm disorders, which are influenced by chronotype. Disrupted sleep rhythms can diminish athletes' capacity to endure exercise loads, highlighting the importance of optimizing sleep management strategies in athletic training programs.

Objective: This study aims to investigate the correlation between the platelet to lymphocyte ratio (PLR) and the occurrence of vascular events in patients undergoing maintenance hemodialysis (MHD). Methods: We collected clinical data from 189 end-stage renal disease patients receiving first and maintenance hemodialysis at the Blood Purification Center of the First Affiliated Hospital of Jinan University between January 1, 2016 and October 31, 2021. Patients were followed up until October 31, 2022, with the occurrence of new cardiovascular events as the study endpoint. Based on the optimal PLR cutoff value of 133.0 determined by X-tile software, patients were categorized into low PLR (<133.0) and high PLR (≥133.0) groups. Baseline characteristics and clinical data correlations were analyzed. Kaplan-Meier curves and log-rank tests evaluated the probability of cardiovascular events between groups. Cox proportional hazards regression models, including inverse probability weighting, identified risk factors for cardiovascular events. Results: Compared to the low PLR group, patients in the high PLR group had a higher prevalence of cardiovascular disease (CVD), elevated blood glucose, C-reactive protein (CRP), and platelet count (P<0.05). PLR was positively correlated with CVD, CRP, and neutrophils (P<0.05). The log-rank test indicated a significant difference in cardiovascular event probability between the high and low PLR groups (log-rank=7.396, P=0.007). Multivariate Cox regression analysis, adjusted for age, history of CVD, diastolic blood pressure, glucose, ALB, and CRP, showed that high PLR is an independent risk factor for CVD incident in MHD patients [HR (95%CI)=1.867 (1.023~3.410), P=0.042]. Univariate Cox regression analysis of the inverse probability-weighted population confirmed that patients in the high PLR group had a 1.9-fold higher risk of cardiovascular events compared to the low PLR group [HR (95%CI)=1.931 (1.033~3.606), P=0.039]. Conclusion: PLR is indicative of inflammatory status and serves as an independent risk factor for cardiovascular events in MHD patients. There may be a causal relationship between elevated PLR and the increased risk of cardiovascular events in MHD patients.

Objective: Familial platelet disorder with a predisposition to acute myeloid leukemia (FPD/AML) is a rare autosomal dominant disorder caused by runt-related transcription factor 1 (RUNX1) genetic variant. This study aimed to explore the clinical phenotypic and RUNX1 genotypic characteristics of a pediatric patient with FPD/AML, who is a three-day-old male, providing evidences for the diagnosis and management of this condition. Methods: Clinical information of the patient was collected and analyzed. Peripheral blood DNA was extracted from the patient and his parents. Next-generation sequencing (NGS) was used to explore the genetic causes. Minigene splice variant analysis was employed to study the aberrant transcript arising from novel splice-site variant. The pathogenicity was assessed according to American College of Medical Genetics and Genomics guidelines. Results: The patient was admitted due to thrombocytopenia uncovered for two days. Laboratory analysis revealed significant thrombocytopenia and dysplasia of megakaryocytes in the bone marrow. Genetic testing identified a heterozygous RUNX1 variant c.509-2A>G, which was not previously reported in any official literatures. On minigene analysis, this variant resulted in the formation of an aberrant transcript r.509_515del (p.Gly170Alafs*3). The patient was clearly diagnosed with FPD/AML. Symptomatic and supportive therapeutics stabilized the platelet count, which remained below the lower limit. The long-term outcome needed to be followed-up. Conclusion: This study identified a novel RUNX1 splice-site variant c.509-2A>G and confirmed its pathogenic role in FPD/AML by using Minigene splicing assay. The findings expanded the RUNX1 variant spectrum and had reference value for the diagnosis and management of FPD/AML.