Objective: To investigate the role and mechanism of cytoskeleton-associated protein 2-like (CKAP2L) in the proliferation, epithelial-mesenchymal transition (EMT), and migration of non-small cell lung cancer (NSCLC) cells. Methods: Potential NSCLC-related genes were screened using the GEO and TCGA databases. qRT-PCR, Western blotting, and immunohistochemistry were applied to detect CKAP2L expression in NSCLC tissues. The survival rate in NSCLC patients was analyzed using the Kaplan-Meier method. After treatment with CKAP2L shRNAs, CKAP2L overexpression plasmids, or Wnt/β-catenin inhibitor IWR-1, CKAP2L expression was measured by qRT-PCR and Western blot. Cell proliferation was assessed by CCK-8 and EdU staining. Cell migration was confirmed using a Transwell assay. Western blotting and immunofluorescence were used to monitor the expression levels of EMT-related and Wnt/β-catenin pathway proteins. Results: CKAP2L was upregulated in NSCLC tissues (P<0.001), and high CKAP2L expression was associated with poor prognosis in NSCLC patients. Compared with those in the overexpression control (OE-CTRL) group, the proliferation (P<0.01 or P<0.001), EMT and migration (P<0.01) of NSCLC cells were significantly enhanced in the CKAP2L-overexpressing (OE-CKAP2L) group; compared with those in the knockdown control (sh-CTRL) group, the proliferation (P<0.01 or P<0.001), EMT and migration (P<0.01) of NSCLC cells were significantly weakened in the CKAP2L-knockdown (sh-CKAP2L) group. CKAP2L upregulated Wnt/β-catenin pathway-related proteins (P<0.01). Conclusion: CKAP2L might accelerate NSCLC progression through the Wnt/β-catenin pathway, suggesting that CKAP2L may be a new target for treating NSCLC patients.

This review summarized the research progress on the biomedical and biomechanical characteristics, influencing the speed ability of elite sprinters, aiming to provide a scientific and comprehensive reference for the assessment and selection of sprinters. The biomedical characteristics of sprinters' speed ability including the related genotyping, body morphology, and physiological functions and etc. The main researches showed: The ACTN3 gene has been confirmed to be closely associated with sprint and jump performance in sprinters; Muscle morphology and volume, as well as muscle fiber type, also have significant impacts on sprint performance; Physiological function monitoring studies have demonstrated that indicators, such as cardiopulmonary function and blood testosterone levels, can reflect athletes' physiological states in real-time and effectively assess training effectiveness. The importance of athletes' biomechanical characteristics in sprinting is also crucial. Research has confirmed that muscle length, maximum elongation of tendons and aponeuroses, and plantar flexor torque are equally key factors determining sprint performance. Furthermore, psychological factors, age, starting techniques, and the scientific training methods collectively influence athletes' performance outcomes.

Objective: To investigate the protective effect of Lycium barbarum extracts (LBE, Ningxia, China) on the retinal structure and function in the mice administrated with optic nerve crush (ONC). Methods: The changes of flash electroretinogram (fERG) and retinal structure in the normal group, ONC-2 d group and ONC-7 d group were compared. Then, the effects of intraperitoneal injection of various dosages of LBE on the survival of retinal ganglion cells (RGCs) in the ONC-7 d group were compared. After that, the LBE dosage with the best protective effect was selected for the next experiment. Finally, we further studied the effects of 1.0 mg/kg LBE on the retinal ERG and structure preservation ONC-7 d group. In all the mentioned experiments, the full-field flash ERG was used to detect the changes in the a-wave and b-wave amplitude and latency under dark and light conditions in the living animals. The collected retinas were processed as the retinal flat whole-mounts and used for the immunofluorescent labeling of the RGCs, or as the frozen sections to be utilized for the fluorescent staining of the retinal bipolar cells, cones, synaptophysin (SYN), anti-postsynaptic density protein 95 (PSD95) and the reactive oxygen species (ROS). ImageJ software was used to count the numbers of the surviving RGCs, bipolar cells and cones, to calculate the percentage of the fluorescence-positive area (PP) of the bipolar cell protrusions in the inner plexiform layer (IPL), to measure the fluorescence intensity (FI) of the SYN in the IPL and outer plexiform layer (OPL), to measure the FI and PP of the PSD95 in the OPL, and to detect the red FI after the DHE detection. Results: Compared with the N and ONC-2 d groups, the numbers of the survival RGCs, bipolar cells and cones, the PP of the bipolar cell protrusion in the INL, and the FI of the SYN in the IPL and OPL decreased (P<0.05) in the ONC-7 d group. The FI of the PSD95 did not change (P>0.05); but the PP decreased in the OPL (P<0.050). The results of ERG examination showed that the amplitudes of a- and b-waves decreased under dark adaptation (P<0.01) and the reduction in the amplitude of b wave during light adaptation was significant 7 days after surgery (P<0.05). DHE staining showed that the ROS was increased 7 days after the operation (P<0.01). Compared with the control group, treatment with LBE could increase the survival of the RGCs (P<0.01) with the largest survival average density of RGCs in the 1.0 mg/kg LBE group (P<0.01). In addition, compared with the control group, 1.0 mg/kg LBE could increase the PP occupied by the bipolar cell protrusions in the IPL (P<0.05), the number of surviving cone cells (P<0.01), the FI of SYN in the IPL (P<0.01) and OPL (P<0.05), and the FI and the PP of PSD95 in the OPL (P<0.05). The improved function of the retina was shown by the increasing amplitudes under dark adaptation (P<0.05) and light adaptation (P<0.01). LBE reduced the FI of ROS (P<0.05). Conclusion: LBE has a protective effect on the retina after ONC injury in mice, and its protective effect may be related to its inhibition of oxidative stress in the retina.

Bone homeostasis is a dynamic homeostasis process maintained by osteocytes, osteoblasts and osteoclasts, which is essential for the growth and development of bone. Protein phosphorylation and dephosphorylation can affect bone formation and development. Protein phosphatase 2A (PP2A), as an important member of the protein phosphatase family of cellular dephosphorylation, contains many subunits, has a complex structure and a wide range of functions. It can dephosphorylate proteins and participate in cellular biological processes like regulation of enzymes, transcription factors and signaling. It also plays a key role in the differentiation process of osteogenesis and osteoclasts. This review summarizes the regulatory role of PP2A in bone homeostasis and the research progress of its various inhibitors in the treatment of bone-related diseases, hoping to provide new ideas and theoretical basis for the drug treatment of bone-related diseases in clinical practice.

Objective: To screen an extract against Streptococcus agalactiae and Streptococcus iniae from Artemisia argyi Levl. et Vant, and to explore its in vitro antimicrobial activity and mechanism. Methods: The agar plate diffusion method was used to screen for Artemisia argyi Levl. et Vant extracts with excellent in vitro antibacterial effects. Subsequently, the minimum inhibitory concentration against Streptococcus agalactiae and Streptococcus iniae was determined. By observing the formation of biofilms, the hydrophobicity and relative conductivity of bacterial surfaces treated with the extract, and the structure of bacterial cells, its antibacterial mechanisms were explored. Results: The selected Artemisia argyi Levl. et Vant extract with good antibacterial effect was Artemisia argyi water extract (AWE). AWE can increase cell membrane permeability by inhibiting the formation of bacterial biofilm, reducing surface hydrophobicity, and increasing relative conductivity, thereby damaging bacterial cell membrane and bacterial structure. Conclusion: AWE is an excellent antibacterial extract of Artemisia argyi Levl. et Vant, which has the potential to serve as a natural antibacterial agent for Streptococcus agalactiae and Streptococcus iniae.

Objective: The interleukin-33 (IL-33)/suppression of tumorigenicity 2 (ST2) signaling pathway plays an important role in the development of tumors. Increased IL-33 levels promote the proliferation of tumor cells. This article aims to screen and express nanoantibodies targeting interleukin IL-33, block the transduction of the IL-33/ST2 signaling pathway by binding to IL-33, regulate inflammatory responses, and prevent tumor deterioration. Methods: Nanoantibody sequences that specifically bind to interleukin IL-33 were screened from a large-capacity natural phage nanoantibody library by panning, enrichment verification and screening, and two Nb_IL-33 nanoantibodies with different amino acid sequences were obtained; the Nb_IL-33 nanoantibody sequence was amplified from the phage plasmid pMECS by PCR technology, and the nanoantibody sequence was connected to the expression vector pMal-c4x by plasmid construction method and transformed into the expression strain BL21 (DE3) to obtain the soluble fusion expression strain of Nb_IL-33 and MBP tag protein: Ni-NTA affinity chromatography was used to purify Nb_A1 fusion protein and Nb_E12 fusion protein; ELISA method was used to detect the specificity, affinity and thermal stability of Nb_IL-33; CCK8 method was used to detect the inhibitory effect of Nb_IL-33 on IL-33-induced human breast cancer cell proliferation. Results: By screening the nanoantibody sequence that specifically binds to interleukin IL-33 from the natural phage nanoantibody library, two Nb_IL-33 nanoantibodies with different amino acid sequences were obtained, named Nb_A1 and Nb_E12 respectively; they were expressed in E.coli BL21, and two nanoantibodies with a purity of more than 90% were obtained by Ni-NTA affinity chromatography. ELISA showed that both Nb_A1 and Nb_E12 could bind to IL-33, and their affinity constants Ka values were (6.068±2.58)×10⁵ mol/L and (2.17±0.37)×10⁶ mol/L, respectively; CCK8 assay proved that both NbA1 and NbE12 had an inhibitory effect on IL-33-induced human breast cancer cell proliferation, and the inhibitory effect of Nb_A1 was greater than that of Nb_E12. Conclusion: In this study, two IL33-targeting nanoantibody sequences were screened from the natural phage nanoantibody library, and the Nb_IL-33 fusion protein expression strain was constructed. Non-fusion proteins Nb_A1 and Nb_E12 with a purity greater than 90% were purified by Ni-NTA affinity chromatography, and their biological functions were preliminarily studied, laying the foundation for the tumor treatment strategy targeting IL-33.

Objective: To isolate terpenoids from supercritical carbon dioxide extracts of Artemisia argyi L. and evaluae their anti-inflammatory and anti-tumour activities, in order to provide a basis for the development of highly effective and low-toxicity anti-inflammatory and anti-tumour drug leads. Methods: The essential oil was extracted by supercritical carbon dioxide extraction technique after crushing the air-dried Artemisia argyi leaves. The crude extracts of essential oil were separated and purified using various separation chromatographic columns (silica gel columns, ODS, MCI, LH-20, semi-preparative HPLC, etc). The structures of the isolated compounds were identified by nuclear magnetic resonance spectroscopy. The anti-inflammatory activity of the compounds on the lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model was determined by the Griess method; the anti-tumor activity of the compounds and their toxicity to normal cells were determined by the CCK8 method. Results: Twenty-eight monomer compounds were isolated from Artemisia argyi leaves, including 3 monoterpenes, 21 sesquiterpenes, 1 diterpene compound and 3 aliphatic alcohols compounds. Compounds 4, 5, 6, 9, 10, 14 and 15 were found to have anti-inflammatory activity, among which compounds 4, 9 and 15 showed significant anti-inflammatory activity, with IC₅₀s of (1.8±0.3) μmol/L, (1.2±0.1) μmol/L, (1.5±0.2) μmol/L, and CC₅₀s of (28.6±1.5) μmol/L, (15.6±0.6) μmol/L, (18.9±0.7) μmol/L. Compounds 4, 5, 6, 9 and 14 possessed anti-tumour activity, of which compounds 4 and 5 were more significant, the IC₅₀s were (5.9±0.6) μmol/L, (6.3±0.1) μmol/L and (13.5±0.9) μmol/L, (20.5±0.6) μmol/L for tumour cells U251 and A549, and the CC₅₀s were (41.0±1.2) μmol/L and (37.4±0.2) μmol/L for normal cells BV2, which provided a certain degree of safety. Conclusion: A total of 28 compounds were isolated from the supercritical carbon dioxide extract of Artemisia argyi L. Compounds 1, 9, 11, 12, 23, and 24 were isolated from Artemisia argyi L for the first time. Compounds 4, 9, and 15 showed significant anti-inflammatory activity, while compounds 4 and 5 had significant anti-tumor activity and had pharmacological value for further study.

Objective: To explore the relationship between dietary inflammation index and the risk of gynecologic malignant tumor. Methods: From January 2001 to March 2022,52 patients with gynecological malignant tumors were selected as case group, and 315 healthy persons were selected as control group. By filling in personal history questionnaire and food frequency questionnaire (FFQ25) to collect general demographic data and dietary intake, the dietary composition was calculated according to Chinese food composition table (2002 Edition), and personal DII values were ultimately calculated. Propensity score matching (PSM) was used to make the baseline data of the cohort similar, and Logistic regression was used to assess the association between DII values and the risk of gynecologic malignancy. To consult the medical examination reports of the two groups, inflammatory markers including neutrophil, lymphocyte, platelets, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and C-reactive protein (CPR) were analyzed. Results: By using multiple regression equation, the scheme of unadjusted confounding variables, scheme of adjusted Ⅰ and scheme of Adjusted Ⅱ showed that the DII value and the high DII score (0.62-4.82) were closely related to the occurrence of gynecological tumors, the difference was significant (P<0.01), and the results of sensitivity analysis were consistent with those before propensity score matching. The relationship between DII value and the occurrence of gynaecological tumors was found by smoothing curve fitting; through threshold analysis, it was found that the inflection point of DII value was 1, and the difference was statistically significant (P<0.01). The levels of inflammatory markers such as NLR, PLR and CPR in the case group were higher than those in the control group, the difference was statistically significant (P<0.01). According to threshold analysis, DII was divided into two groups, the levels of inflammatory markers in DII ≥1 group were significantly higher than those in DII<1 group (P<0.01). Conclusion: DII value has a curve relationship with the occurrence of gynecological tumors, and DII value can be used as a tool to predict the risk of gynecological tumors. When the DII value is higher than 1, the occurrence and development of gynaecological tumors need to be highly vigilant. The DII value can be used to guide the dietary structure of residents and carry out relevant nutrition education, so as to prevent the occurrence and development of gynecological tumors.

Objective: To analyze the chemical components of Artemisia argyi essential oil(AAEO) extracted by supercritical carbon dioxide combined with molecular distillation and evaluate its antibacterial, anti-inflammatory and tyrosinase inhibitory activities, and to discuss the application of AAEO in external preparation. Methods: AAEO was extracted by supercritical carbon dioxide combined with molecular distillation. The chemical components of AAEO were analyzed by gas chromatography-mass spectrometry (GC-MS). The inhibition zone diameter of AAEO against five test bacteria was tested by filter paper diffusion method, including Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested by broth microdilution method. The lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model was used to investigate the effect of AAEO on the mRNA expression levels of inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and inducible nitric oxide synthase (iNOS). The effect of AAEO on tyrosinase activity and melanin production was evaluated by human melanoma cell (A375 cell) model. Results: A total of 132 compounds were identified from AAEO, the main components of which were α-terpineol (7.08%), terpinen-4-ol (6.45%), β-caryophyllene (5.83%), eucalyptol (4.82%), endo-borneol (4.54%), 2-cyclohexen-1-ol (3.82%), d-camphor (3.09%), carveol (1.49%), and α-thujone (0.75%). AAEO showed significant antibacterial activity against the five tested bacteria, among which Candida albicans was highly sensitive. The MICs against Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans were 1.88, 3.75, 7.50, 15.00, 0.94 mg/mL, and the MBCs were 1.88, 7.50, 15.00, 30.00, 1.88 mg/mL respectively. AAEO could reduce the mRNA expression levels of IL-1β, IL-6, TNF-α, iNOS in RAW264.7 cells significantly (P<0.05). AAEO could inhibit tyrosinase activity in A375 cells significantly (P<0.05), and the production of melanin was reduced significantly (P<0.05). Conclusion: AAEO extracted by supercritical carbon dioxide combined with molecular distillation are rich in chemical components and has good antibacterial, anti-inflammatory and tyrosinase inhibitory activities. Its application value is worthy of in-depth study.

Objective: To isolate guaiane-type sesquiterpenes from leaves of Artemisia argyi H. Lév. & Vaniot, providing scientific evidence for the development and utilization of sesquiterpene compounds found in Artemisia argyi leaves. Methods: Compounds were isolated using conventional open-column chromatography and semi-preparative high-performance liquid chromatography. The structures of the compounds were elucidated by modern spectroscopic techniques. The cytotoxicity of the compounds was assessed by CCK-8 assay, and their inhibitory activity against nitric oxide (NO) production was evaluated by Griess reagent. Results: Ten guaiane-type sesquiterpenes were isolated and identified, comprising 2 unknown compounds (1-2) and eight known compounds (3-10). Compound 4 significantly inhibited NO production in LPS-stimulated RAW264.7 cells, with a half-maximal inhibitory concentration (IC₅₀) of (1.5±0.1) μmol/L. Conclusion: Notable anti-inflammatory activity has been identified in sesquiterpene compounds present in Artemisia argyi leaves. The identification of these novel sesquiterpenes enhances the chemical profile of Artemisia argyi leaves and offers strong scientific support for their potential applications in the pharmaceutical industry.

Objective: To investigate renal ischemia-reperfusion (I/R) injury inducing Parthanatos in renal tubular epithelial cells and the upstream signals that initiate this death pathway's activation. Methods: A mouse model of renal I/R injury and a hypoxia/reoxygenation injury model of renal tubular epithelial cells were established and pretreated with Poly ADP-ribose polymerase 1 (PARP-1) inhibitor 3-aminobenzamide (3AB) and antioxidant N-acetyl-L-cysteine (NAC). Hematoxylin and eosin (HE) staining was used to observe renal tissue pathological damage. Serum creatinine (Cre) and blood urea nitrogen (BUN) were detected to evaluate renal function. The cell counting kit-8 (CCK-8) and lactate dehydrogenase (LDH) assays were used to detect cell viability and mortality. 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) was used to detect intracellular reactive oxygen species (ROS). The activities of superoxide dismutase (SOD) and the levels of malondialdehyde (MDA) in cells were detected using corresponding kits. Enzyme-linked immunosorbent assay (ELISA) and Western blot were applied to detect DNA damage markers and the expression of Parthanatos-related proteins. Results: 3AB pretreatment alleviated I/R-caused renal tissue damage and renal dysfunction (P<0.05), and reduced hypoxia-reoxygenation-induced cell death (P<0.05). NAC pretreatment decreased the levels of intracellular ROS and MDA in hypoxia-reoxygenation cells (P<0.05), increased the activity of SOD (P<0.05), inhibited DNA damage and Parthanatos pathway activation (P<0.05), reduced cell death (P<0.05), and alleviated renal injury and renal dysfunction (P<0.05). Conclusion: I/R-caused oxidative stress can lead to DNA damage and activate PARP-1, which in turn promotes the occurrence of Parthanatos in renal tubular epithelial cells. This provides new ideas for the effective prevention and treatment of renal I/R injury.

Autoimmune glial fibrillary acidic protein astrocytopathy (A-GFAP-A) is a rare autoimmune encephalitis discovered in the last decade. The diagnosis depends on clinical symptoms, imaging, and antibody testing. Most cases require several months or even longer to make a definite diagnosis. Due to early-stage diagnosis of this condition poses significant challenges, and there is a lack of relevant clinical guidelines, there are few reports at home and abroad on early diagnosis and treatment in such cases. This paper reported a case of high fever and headache as the initial symptoms, accompanied by persistent hyponatremia. With the help of ¹⁸F-fludeoxyglucose-positron emission tomography/magnetic resonance (¹⁸F-FDG PET/MR) imaging combined with antibody testing, the patient was diagnosed with anti-GFAP encephalitis approximately one month after symptom onset, and evaluated the patient's immunotherapy efficacy, leading to a favorable prognosis for the patient. This case provides a certain reference for the early clinical diagnosis and treatment of such patients.

Objective: This study aims to explore the characteristic genes and potential mechanisms of ferroptosis-related idiopathic pulmonary fibrosis (IPF). Methods: To construct a ferroptosis-related IPF risk model (Fer-Score) by using WGCNA and ssGSEA. Subsequently analyzed immune cell infiltration and pathway enrichment in different risk subtypes. The LASSO algorithm was employed to screen ferroptosis-related genes characteristic of IPF and assess the correlation between immune cell infiltration abundance, key pathway scores and characteristic genes. Finally, we validated the expression of characteristic genes and conducted functional studies using an IPF cell model. Results: Nine ferroptosis-related IPF genes were identified for constructing the Fer-Score. The risk scores of different subtypes of Fer-Score showed significant difference, with the model achieving an area under the ROC curve of 0.917 7 and 0.937 5 for the discovery and validation sets, respectively. Immune infiltration analysis revealed significant correlations between enrichment scores and memory B cells, dendritic cells, resting NK cells, monocytes, and neutrophils. Pathway enrichment analysis identified 24 pathways significantly enriched by GSEA, seven of which were notably related to immune cell infiltration. Combined LASSO algorithm and Pearson correlation analysis identified MEG8, ALOX15, and ADAM23 as characteristic genes contributing to IPF. Cell experiments showed that ADAM23 was significantly overexpressed in the IPF cell model, and knockdown of ADAM23 inhibited LPS induced proliferation and migration of BEAS-2Bs cells. Conclusion: Knockdown of ADAM23 can inhibit the progression of IPF potentially by regulating metabolism-related pathways to influence the infiltration patterns of five types of immune cells. Thereby ADAM23 may contribute to the occurrence of IPF.

Objective: To evaluate the clinical efficacy of intelligent controlled pressure ureterorenoscopy in treating polycystic kidney disease with renal calculi. Methods: A retrospective analysis was conducted on the clinical data of three patients with polycystic kidney disease and renal calculi treated in the first affiliated hospital of Jinan University between June 2022 and September 2023. Intelligent controlled pressure ureterorenoscopy was performed, and the treatment outcomes and incidence of complications were analyzed. Results: All three patients were male, with an average age of (51.00±6.08) years old. The average surface area of the stones in (4.75±4.53) cm². Two patients had stones on the left side, and one had stones on the right side. All three patients exhibited varying degrees of renal hydronephrosis. All three surgeries were successfully completed. The average operative time was (195.30±65.61) min. No active bleeding occurred during or after the surgery. None of the patients experienced complications such as sepsis, organ injury, or irrigation fluid absorption syndrome. Routine blood and biochemical tests were conducted on the first day post-surgery. The average decrease in hemoglobin was (9.73±13.35) g/L, and no patient required blood transfusion. The average postoperative serum creatinine level was (265.10±194.28) μmol/L. Conclusion: Intelligent controlled pressure ureterorenoscopy is a safe and effective treatment for polycystic kidney with renal calculi.

Objective: To investigate the active anti-neuroinflammatory components and mechanisms of Artemisia vulgaris L. from Tangyin, providing a theoretical basis for the discovery of lead compounds and the development of anti-neuroinflammatory drugs from this plant. Methods: Terpenoids from Artemisia vulgaris L. were isolated using open column chromatography and high-performance liquid chromatography (HPLC), and their structures and configurations were identified using nuclear magnetic resonance (NMR) spectroscopy. The CCK8 method was utilized to assess the effect of compounds on cellular proliferation capacity. The content of NO in cells was detected by the Griess assay, while the mRNA expression levels of inflammatory cytokines TNF-α, IL-6, and IL-1β were assessed by RT-qPCR. Protein levels of TNF-α and IL-6 were determined by ELISA. Furthermore, Western Blot analysis was employed to determine the expression levels of relevant proteins within the PI3K/AKT/mTOR and NF-κB signaling pathways. Results: Nineteen compounds were isolated and identified from the active fraction of Artemisia vulgaris L. with anti-neuroinflammatory activity. Among them, artemvulactone U(AU) had no obvious inhibitory effect on the growth of BV-2 cells, as determined by the CCK-8 assay. Notably, AU also demonstrated the most significant inhibition of NO production, as evidenced by the Griess assay. RT-qPCR and ELISA results indicated that AU suppressed the LPS-induced expression of inflammatory cytokines TNF-α, IL-6, and IL-1β. Western blot analysis revealed that AU alleviated neuroinflammation by downregulating the expression of INOS and COX-2. Furthermore, Western blot analysis suggesting that its anti-inflammatory activity may be mediated through modulation of the PI3K/AKT/mTOR and NF-κB signaling pathways.Conclusion: The compound artemvulactone U isolated from the petroleum ether fraction of Artemisia vulgaris L.has good anti-neuroinflammatory activity and holds the potential for further research and development as an anti-inflammatory agent.

Obesity is a global epidemic and the proportion of obese and overweight people is increasing. Obesity is a risk factor for a variety of non-infectious diseases involving multiple organs throughout the body, including the respiratory system. The effects of obesity on lung function are characterized by mechanical compression of the lungs and low-grade inflammatory effects on the bronchi. Mechanical compression is mainly manifested by decreased respiratory compliance, altered lung volumes, increased airway resistance, altered regional lung ventilation and lung ventilation/perfusion, and respiratory muscle overload. A low-grade inflammatory state can cause inflammatory changes in the bronchial wall and induce airway remodelling. Body mass index (BMI) is a simple and convenient way to categorise the degree of obesity, but does not account for patterns of regional fat distribution. Different patterns of fat distribution have different effects on lung function. Lung function tests can non-invasively and indirectly show changes in lung function in obese people, but they reflect changes in the respiratory system as a whole and cannot directly and dynamically show changes in lung function. CT and MRI imaging tests, can not only accurately calculate the fat content and distribution, but also quantitatively analyse the changes in the lung structure, and more importantly, can also observe the dynamic changes in the thorax, lungs, and respiratory muscles under free breathing, so as to detect the changes in the lung function as early as possible, and thus carry out weight loss interventions in time. This paper will discuss the pathophysiological mechanisms of obesity-induced lung changes; the progress of obesity-related chest imaging and future research hotspots.

Currently, the incidence and mortality rates of tumors are increasing. Traditional tumor diagnosis and treatment methods have many limitations, while photodynamic therapy (PDT), as an emerging tumor treatment method, has attracted more and more attention due to its unique advantages. PDT relies on the photophysical and photochemical actions of photosensitizers under specific wavelengths of light to produce a series of reactive oxygen species (ROS) that induce tumor cell death. Corrole, due to its unique optical properties, selectivity for tumor tissues, rich potential for chemical modification, and good biocompatibility, has broad application prospects in photodynamic therapy. This article provides a systematic review of the synthesis of corrole-based compounds and their progress in tumor treatment.

Jintiange capsule is principally composed of bionic tiger-bone powder. Significant therapeutic effects have been demonstrated in areas including the enhancement of osteogenic activity, increase in bone density, inhibition of bone resorption, and reduction of bone loss, with positive therapeutic impacts observed on various clinical manifestations caused by osteoporosis. When combined with other medications for primary or secondary osteoporosis treatment, enhanced therapeutic efficacy has been documented. Bone mineral density and osteocalcin levels have been effectively improved through this combination therapy. The bone metabolism process has been regulated, patient pain has been significantly alleviated, and favorable safety profiles have been maintained. To evaluate the clinical value of Jintiange capsule combination therapy in osteoporosis treatment, relevant application studies are systematically reviewed in this article.

Essential oil is the main pharmacodynamic substance of Artemisia argyi. Thus, the quality control of Artemisia argyi essential oil is crucial for its stable therapeutic effect. At present, the extraction methods of Artemisia argyi essential oil include steam distillation, supercritical CO₂ extraction, organic solvent extraction, ultrasonic-assisted extraction, etc. This review summarized quality control and extraction technology of Artemisia argyi essential oil in recent years, in order to provide scientific basis for the comprehensive development and application of Artemisia argyi.

Radiation retinopathy (RR) is primarily treated with intravitreal injections of anti-vascular endothelial growth factor (anti-VEGF), which is costly and requires repeated administration. Currently, there is no unified standard for the treatment of RR. This paper reports a case where anti-VEGF therapy was ineffective in Guangdong Provincial People's Hospital in 2018, but 577 nm subthreshold micropulse laser (SML) treatment successfully resolved the severe macular edema caused by radiotherapy for nasopharyngeal carcinoma, with stable therapeutic effects maintained over a 5-year follow-up period without recurrence. There have been no domestic reports on the use of SML treatment for RR macular edema. By reviewing the existing literature, SML is considered to be a cost-effective and non-invasive, allowing for repeatable procedures, it can provide a new strategy for the treatment of RR.

Objective: To investigate the changes in systemic immune inflammatory index (SII), D-dimer level and SOFA score in patients with sepsis and their prognostic value assessment. Methods: Retrospectivly analyzed 114 patients with sepsis hospitalized in the Second Affiliated Hospital of Xinjiang Medical University during the period from July 2021 to July 2023. 43 patients who underwent health checkups during the same period were selected as the control group. Laboratory tests such as coagulation series and blood specimens were performed after admission to the hospital to derive SOFA score. All sepsis patients were followed up for 28 d. Based on the clinical outcomes during hospitalization, the patients were divided into 37 cases in the survival group and 77 cases in the death group. The differences between SII, D-dimer level and SOFA score in the three groups were compared. Univariate and multivariate Logistic regression were used to analyze the baseline data of sepsis patients, to derive the independent factors influencing the death of sepsis patients, and to draw forest plots; Spearman correlation analysis of SII index, D-dimer level and SOFA score with prognosis of patients with sepsis; survival curves were plotted by ROC curves assessing SII, D-dimer level, SOFA scores and the combination of all three. Finally, calibration curves and clinical decision curves were plotted based on the R after the model was re-sampled 500 times using the Bootstrap method. Results: Patients in the sepsis death group had significantly higher SII, D-dimer, and SOFA score than those in the survival and control groups, and the differences were statistically significant. The case fatality rate of patients with sepsis was 68%. Analysis of one-way Logistic regression showed that SII, D-dimer level, and SOFA score were correlates of death in sepsis patients. Multifactorial Logistic regression analysis showed that high SII, high D-dimer level, and high SOFA score were independent risk factors for death in patients with sepsis. SII, D-dimer level, and SOFA score were positively correlated with death in patients with sepsis. The results of the ROC curve analysis showed that the area under the curve of SII, D-dimer level, SOFA score, and the combined test to predict death in patients with sepsis, were 0.822, 0.888, 0.874, 0.967 respectively. The diagnostic sensitivity was 73.700% and specificity was 75.000% at the optimal cut-off value of 2 063.5 for SII. The diagnostic sensitivity was 85.500% and specificity 80.600% at the optimal cut-off value of 3.8 mg/mL for D-dimer level. The diagnostic sensitivity was 75.500% and specificity was 86.100% at the cut-off value of 9.500 points for SOFA score. The sensitivity of the combined test was 93.400% and the specificity was 91.700%. Conclusion: Elevated levels of SII, D-dimer and SOFA score are independent risk factors for death in sepsis patients, the constructed prediction model has high goodness of fit and clinical applicability, and the combination of the three tests has higher predictive value for sepsis patients.

Objective: To compare the efficacy of transcatheter arterial chemoembolization (TACE) combined with hepatic arterial infusion chemotherapy (HAIC) versus TACE alone in the treatment of advanced hepatocellular carcinoma (HCC). Methods: Retrospective clinical data from 156 patients diagnosed with advanced HCC and treated either with TACE combined with HAIC (55 cases) or TACE alone (101 cases) in the First Affiliated Hospital of Jinan University between March 2018 and October 2021 were collected. Propensity score matching (PSM) was employed to minimize between-group differences. Overall survival (OS), progression-free survival (PFS), tumor response rate, and adverse reaction rates were compared. Results: In the pre-adjusted PSM cohort, the TACE-HAIC group demonstrated significantly longer median OS (19.7 months vs. 9.8 months, P=0.010) and median PFS (10.7 months vs. 5.1 months, P=0.008) compared to the TACE alone group. Although the objective response rate (ORR) did not significantly differ between groups (23.6% vs. 20.2%, P=0.303), the disease control rate (DCR) was higher in the TACE-HAIC group (72.7% vs. 65.8%, P=0.024). After 1∶1 propensity score matching, a matched cohort of 78 cases showed that the TACE-HAIC group had significantly better median OS (19.1 months vs. 7.4 months) and median PFS (10.7 months vs. 5.0 months) than the TACE alone group (P<0.05). The combined treatment also exhibited advantages in overall response rate and DCR, with comparable incidence of adverse reactions between groups (P>0.05). Conclusion: The combination of TACE and HAIC offers superior survival benefits and tumor response rates compared to traditional TACE alone in treating advanced HCC, with good tolerability.

Objective:To investigate the current status of oral health knowledge, attitude and practice (KAP) among elderly individuals aged 60 years and above in a community in Nanning City, and to explore the factors influencing these, in order to provide a reference for the development of oral health management programs for the elderly. Methods:A cross-sectional study was conducted using convenience sampling to select 488 elderly individuals from two communities in Qingxiu District, Nanning City, based on inclusion and exclusion criteria. A questionnaire survey was administered, and SPSS 26.0 was used to perform descriptive analysis on general information and the oral health KAP of the elderly, utilizing frequency, percentage, mean, and standard deviation. Two independent sample t-tests, one-way analysis of variance, and multiple stepwise regression analysis were used to explore the factors influencing oral health KAP among the elderly. Results:The scores for oral health knowledge, attitude, and practice among the elderly were (10.00±4.39), (8.05±3.64), and (22.31±7.58), respectively. Single-factor analysis showed statistically significant differences in oral health KAP scores based on age, education level, and personal monthly income (P<0.05). Multiple stepwise regression analysis revealed that higher education level, younger age, and higher monthly income were associated with better oral health KAP scores. Additionally, elderly individuals with a spouse had significantly higher attitude scores compared to those without a spouse. Conclusion:The oral health knowledge, attitude, and practice of elderly individuals in this community in Nanning City are at a relatively low level and are significantly influenced by factors such as age, education level, and personal income.

Objective: To compare and analyze the anti-atopic dermatitis active components and mechanisms of action of Artemisia argyi essential oil extracted by water distillation and supercritical CO₂ fluid extraction based on network pharmacology and in vitro cell experiments. Methods: The essential oils of Artemisia argyi were extracted by water distillation and supercritical CO₂ fluid extraction, and their chemical components were determined by gas chromatography-mass spectrometry. Network pharmacology was used to predict the related targets and pathways of the two essential oils against atopic dermatitis, and the anti-atopic dermatitis activities of the two essential oils were explored in the lipopolysaccharides (LPS)-induced RAW264.7 cell inflammation model. Results: There were 65 compounds in Artemisia argyi essential oil obtained by steam distillation (AEOSD), 89 compounds in Artemisia argyi essential oil obtained by supercritical fluid extraction (AEOSFE), and 44 common compounds in the two essential oils. Network pharmacology analysis results found that eucalyptol and humulene were the main potential active ingredients of AEOSD against atopic dermatitis, eugenol, eucalyptol, and Copaene were the main potential active ingredients of AEOSFE against atopic dermatitis. AEOSD may affect the progression of atopic dermatitis by regulating the biosynthesis of nitric oxide (NO), the positive regulation of interleukin-6 (IL-6) production, and the NOD-like receptor signaling pathway, while AEOSFE may affect the progression of atopic dermatitis by regulating the biosynthesis of NO, T helper cell 17 (Th17) cell differentiation, PI3K-Akt signaling pathway, and inflammatory mediator regulation of TRP channels. In vitro experimental results showed that both AEOSD and AEOSFE could reduce the excessive release of NO in RAW264.7 cells induced by LPS, and reduce the expression of their target inflammatory factors IL-1β, IL-6, TNF and IL-1β, IL-6, PTGS2, respectively. Conclusion: AEOSD and AEOSFE differ in their chemical composition and target pathways against atopic dermatitis, but both have good anti-inflammatory activity on RAW264.7 cell.

Objective: To investigate the clinical characteristics of fibromyalgia syndrome (FMS) complicated by migraine. Methods: The study included 210 FMS patients who were treated at the Third Affiliated Hospital of Qiqihar Medical University from January 2021 to December 2023. General and clinical data were collected. Assessment was conducted using scales such as the wide spread pain index (WPI), symptom severity scale (SSS), fibromyalgia severity (FS), pain score, anxiety score, sleep score, and somatic symptom score. Results: Among the 210 FMS patients, 108 (51.43%) had concomitant migraine. Compared with FMS patients without migraine, those with migraine had significantly higher scores in FMS duration (P=0.001), WPI score (P=0.003), SSS score (P=0.001), FS score (P=0.002), pain score (P=0.008), anxiety score (P=0.005), sleep score (P=0.004), and somatization symptom score (P=0.009). In FMS patients with migraine, FS scores were positively correlated with headache duration (P=0.013), headache frequency (P=0.019), headache duration (P=0.037), FMS duration (P=0.025), pain score (P=0.028), anxiety score (P=0.030), sleep score (P=0.035), and somatization symptom score (P=0.008). Conclusion: The presence of migraine exacerbates the clinical symptoms of FMS, and there is a correlation between FMS symptoms and headache symptoms, anxiety, and sleep disturbances when FMS is complicated by migraine.

Objective: To explore the relationship between triglyceride-glucose index (TyG) and all-cause mortality in patients with acute myocardial infarction (AMI). Methods: The data for this study came from the ICU Information Mart for Intensive Care (MIMIC) database. The clinical outcome is hospitalized mortality. The relationship between TyG index and hospitalized mortality was analyzed using restricted cubic spline (RCS) model, and the Cox proportional hazards model was used to evaluate the association between different TyG groups and in-hospital all-cause mortality. Results: A total of 501 patients were included in the study, including 170 in the low TyG group (TyG<8.80), 167 in the medium TyG group (8.80≤TyG<9.35), and 164 in the high TyG group (TyG≥9.35). With the middle TyG group as the reference group, in univariate analysis, the hazard ratio (HR) of the low TyG group was 1.84(95%CI: 0.68~4.98, P=0.229), the HR of the high TyG group was 3.02(95%CI: 1.19~7.65, P=0.02); in Model Ⅰ with adjusted variables, the HR of the low TyG group was 1.91(95%CI: 0.70~5.23, P=0.209), the HR of the high TyG group was 3.68 (95%CI: 1.44~9.42, P=0.007); in further adjusted Model Ⅱ, the HR of the low TyG group was 4.47(95%CI: 2.17~9.21, P<0.001), the HR of the high TyG group was 2.00(95%CI: 1.01~3.94, P=0.045). Conclusion: The research results indicate that TyG index, as a simple and easily accessible laboratory parameter, has important value in evaluating the prognosis of patients with acute myocardial infarction. A higher TyG index is significantly correlated with an increase in in-hospital mortality.

Objective: To explore the correlation between C-reactive protein clearance rate (CRPc) and airway hyperresponsiveness (AHR) in acute bronchitis patients, in order to provide a reference for clinical diagnosis of AHR degree in acute bronchitis patients. Methods: A total of 182 patients with acute bronchitis admitted to Luzhou Hospital of Traditional Chinese Medicine from June 2020 to June 2023 were selected as the study objects, and were divided into group A: mild (n=67), group B: moderate (n=59), and group C: severe (n=56) according to the different degrees of AHR. The method of 1∶1 orientation matching was used to adjust the balance of patient data. All patients underwent lung examination, fasting blood was collected in the morning, and serum indexes of patients were detected by enzyme-linked immunosorbent assay. CRP level and CRPc were detected within 24 h and after admission and 3, 5, and 7 d after treatment. Multivariate Logistic regression analysis was conducted to analyze the correlation between CRPc and AHR degree, analyze the clinical factors affecting AHR degree, construct the corresponding prediction model and evaluate. Results: After 1∶1 orientation matching, 47 patients with mild, moderate and severe AHR with no statistical difference in various indicators before treatment were obtained. After treatment, the levels of FEV₁/FVC, MVV, interferon-γ (IFN-γ), IL-10 and PD₂₀FEV₁ in the 3 groups were increased, while the levels of CRP, YKL-39 and LTE4 were decreased, indicating a significant improvement effect. In addition, the levels of FEV₁/FVC, MVV, IFN-γ, IL-10 and PD₂₀FEV₁ in group A were higher than those in the other two groups, while the levels of CRP, YKL-39 and LTE4 were lower than those in the other two groups, and the improvement effect was better. The CRPc of group A, B and C after 7 d of treatment were (70.66±14.85)%, (60.55±15.52)% and (48.24±14.47)%, respectively, and the CRPc of group A were significantly higher than those of the other two groups. Multivariate Logistic regression analysis showed that CRPc-3 (OR=0.85, 95%CI: 0.69-0.87), CRPc-5 (OR=0.92, 95%CI: 0.77-0.95) and CRPc-7 (OR=0.96, 95%CI: 0.89-0.98) were correlated with the degree of AHR in patients. YKL-39 and LTE4 are risk factors (OR>1) for developing severe AHR, while CRPc-3, CRPc-5, CRPc-7, IFN-γ and PD₂₀FEV₁ are protective factors (OR<1) for developing severe AHR. All 7 factors have high predictive value for the risk of developing severe AHR, but the combined detection has the highest predictive value. A model built by combining the above 7 factors: Logit(P)=-2.036-0.449 (CRPc-3)-0.507(CRPc-5)-0.114(CRPc-7)-0.464(IFN-γ)+0.449(LTE4)+0.622(YKL-39)-0.200(PD₂₀FEV₁). When P=0.90, the Yoden index is the highest and the prediction effect is the best with prediction accuracy 83.10%, sensitivity 93.71%, and specificity 78.52%. Conclusion: Patients with mild AHR had higher CRPc than those with moderate and severe AHR, CRPc-3, CRPc-5 and CRPc-7 were correlated with the degree of AHR.

Objective: To explore the distribution pattern and related influencing factors of dampness syndrome in patients with precancerous lesions of gastric cancer (PLGC) through epidemiological investigation. Methods: Epidemiological data of PLGC patients who visited Guangdong Provincial Hospital of Chinese Medicine from June 2023 to November 2023 were collected. After grouping them into different dampness syndrome types, analysed the effects of social demographics data, lifestyle, and disease conditions on PLGC patients with different dampness syndrome types. Finally, the influencing factors with statistical difference were analysed by unordered multiclassified Logistic regression analysis. Results: A total of 197 PLGC patients were collected in this study, including 35 patients with non-dampness syndrome, 30 patients with dampness-heat syndrome, 87 patients with spleen-dampness syndrome, 17 patients with wind-dampness syndrome, and 28 patients with at least 2 different types of dampness syndrome. Correlation analysis of non-dampness syndrome group, dampness-heat syndrome group, spleen-dampness syndrome group, and wind-dampness syndrome group revealed that there were statistical differences among the four groups in depression, dietary habits, food intake, smoking history, and staying up late. Logistic regression analysis of the above factors revealed that depression and having smoking history were independent influencing factors of dampness-heat syndrome, and staying up late was an independent influencing factor of spleen-dampness syndrome. Conclusion: Dampness syndrome was widely present in PLGC patients, and depression and a history of smoking may independently affect dampness-heat syndrome in PLGC, while staying up late may independently affect spleen-dampness syndrome in PLGC.

Objective: To screen the compounds with high inhibitory potency on influenza virus from Traditional Chinese Medicine (TCM) extracts. Methods: Neuraminidase (NA) inhibitory activity assay was used to screen 148 TCM extracts, among which Curcuma longa L. extract displayed the best NA inhibitory activity. The bioactive compounds were isolated from the extract of Curcuma longa L. using at-line nanofractionation(ANF). The structures of these compounds were analyzed using mass spectrometry and their interactions toward NA were examined through molecular docking. Results: NA inhibitory rate of the extract from Curcuma longa L. was found to be 92%. A total of eight bioactive compounds, mainly curcumin analogues, were identified from Curcuma longa L. extract, indicating curcuminoids play a significant role for NA inhibition. Curcuminoids have a different mechanism of action compared to oseltamivir. Conclusion: ANF technology can play an important role in the screening of neuraminidase inhibitors. This finding not only provides a scientific basis for the potential clinical use of Curcuma longa L. against the influenza virus but also establishes a foundation for the development of novel influenza antivirus drugs.

Objective: To compare the predictive value of decision tree and Logistic regression model for pregnancy outcomes in in vitro fertilization-embryo transfer (IVF-ET) patients. Methods: A total of 350 patients undergoing IVF-ET in Heping Hospital of Changzhi Medical College from January 2021 to October 2022 were included in the study, and were divided into pregnancy success group (215 patients) and pregnancy failure group (135 patients) according to pregnancy outcome. Clinical data of patients were collected, Logistic regression and decision tree models were established to predict pregnancy outcomes of IVF-ET patients, and decision tree analysis models (decision tree 1 and decision tree 2) were established under the condition of whether the results of Logistic regression were based, and receiver operating characteristic (ROC) curve was used to evaluate the prediction effect of the model. Results: Of the 350 patients, 61.43% had successful pregnancy, and the 38.57% had failed pregnancy. In the pregnancy failure group, the age ≥35 years, infertility years ≥5 years, cycle times ≥1, the proportion of patients with mental disorders and serum progesterone level on HCG day were higher than those in the pregnancy success group, and the proportion of patients with egg number ≥10, fertilization rate ≥75%, endometrial thickness on HCG day and high-quality embryo number were lower than those in the pregnancy success group (P<0.05). Multivariate Logistic regression analysis showed that age, serum progesterone level on HCG day, number of high-quality embryos and mental disorders were all influencing factors of pregnancy outcome in IVF-ET patients (P<0.05). Decision tree model showed that age, serum progesterone level on HCG day and number of high-quality embryos were influencing factors of pregnancy outcome in IVF-ET patients. The AUC of Logistic regression model was 0.832, and the prediction sensitivity, specificity and accuracy were 87.3%, 71.4% and 83.5%, respectively. The AUC of decision tree 1 was 0.859, and the prediction sensitivity, specificity and accuracy were 85.1%, 76.8% and 85.6%, respectively. The AUC of decision tree 2 was 0.820, and the prediction sensitivity, specificity and accuracy were 83.7%, 73.2% and 82.4%, respectively. The AUC of decision tree 1 was greater than that of decision tree 2 (P<0.05), but there was no statistical significance compared with that of Logistic regression model (P>0.05). Conclusion: Both Logistic model and decision tree model have certain predictive value for pregnancy outcome of IVF-ET patients.

Objective: To investigate the efficacy and safety of different stimulus dosage of modified electroconvulsive therapy (MECT) in the treatment of depressive episode. Methods: Sixty-five patients with depressive episode according to Diagnostic and Statistical Manual of Mental Disorders, 4th edition (DSM-IV) were randomly divided into treatment group (32 patients) and control group (33 patients). The patients in the treatment group received low stimulus dosage of MECT while the patients in the control group were treated with routine stimulus dosage of MECT. The treatment session was 6 times in both groups. The stimulus electrodes were placed on bifrontal. Hamilton depression scale-17 item (HAMD-17) and MATRICS consensus cognitive battery (MCCB) were used to evaluate the clinical symptoms and cognitive function at baseline, after 3 times of MECT, and after 6 times of MECT. Heart rate, systolic blood pressure, and diastolic blood pressure were recorded before and after stimulus in the first session of MECT treatment. Adverse reactions related to MECT were also recorded. Results: Fifty-six (29 patients in treatment group and 27 patients in control group) completed MECT treatment. The improvement rate of treatment group and control group was 68.97% and 70.37%, the remission rate was 51.72% and 62.96%, respectively. There was significant decrease of HAMD-17 score after MECT compared with baseline in both groups while there was no significant difference between groups. Compared with baseline, the heart rate, systolic pressure, and diastolic pressure increased significantly after stimulus in the first MECT session, while there was no significant difference between groups. There was no significant difference of MCCB score after MECT treatment compared with baseline and between groups. One patient experienced delirium in the treatment group, while in the control group, three patients experienced MECT related side effects involving headache, nausea and vomiting, muscle pain, and delirium. Conclusion: The efficacy of low stimulus dosage MECT treatment was equivalent to the conventional stimulus dosage in treating depressive episodes while with fewer adverse reactions.

Objective: Preliminary construction of a comprehensive scoring system based on qualitative indicators of ^99mTc-SC lymphoscintigraphy to explore the predictive value of ^99mTc-SC lymphoscintigraphy in assessing the efficacy of comprehensive decongestive therapy for secondary lower limb lymphedema following gynecological cancer surgery. Methods: A retrospective analysis was conducted on the data of 48 gynecological cancer patients at the First Affiliated Hospital of Sun Yat-sen University from January 2018 to March 2023 who developed secondary lymphedema following surgical procedures and received comprehensive decongestive therapy. Subsequently, the patients were stratified into effective (n=28) and ineffective treatment (n=20) groups based on predefined research criteria, with both clinical information and ^99mTc-SC lymphography data collected. The qualitative indicators of lymphography primarily encompassed: ① visibility of main lymphatic vessels' uptake pattern; ② identification of obstruction site in the pelvis or knee joint; ③ classification of subcutaneous soft tissue drainage type in lower limbs; ④ assessment of collateral lymphatic circulation presence. Results: There was a statistical difference in the delayed phase of lower limb lymphatic drainage and comprehensive score between the two groups (P<0.05). The predictive performance of the comprehensive score is significantly superior to that of the single qualitative indicator of lymphoscintigraphy. The sensitivity, specificity, and accuracy of prognostic assessment based on the comprehensive scoring system derived from nuclear lymphatic imaging were 96.4%, 85.0%, and 91.7%, respectively. Conclusion: Preoperative lymphoscintigraphy may be valuable for predicting the response to treatment of gynecologic cancer-related lymphedema following comprehensive decongestive therapy. The late-stage soft tissue bypass classification and comprehensive score serves as the primary independent predictor, and a scoring system that integrates multiple qualitative features can significantly enhance the sensitivity and accuracy of prognosis prediction.

Objective: To evaluate the feasibility and the superiority of AI-assisted compressed sensing (ACS) in knee joint MRI. Methods: Thirty-six participants who underwent both the conventional scanning and ACS scanning of knee joint MRI in Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology during the period of 2021 to 2022 were included in this study. The acquisition time of each scanning sequence, selected the bone marrow, muscle, anterior cruciate ligament and posterior cruciate ligament as regions of interest on coronal PD images, and calculated the signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) of these region of interst (ROI) were recorded. We evaluated the subjective image quality, and compared the objective image quality. The Wilcoxon signed-rank test was used to compare the objective image quality, and the kappa coefficient was used to statistically analyze the subjective scores. Results: Compared with conventional group, the total acquisition time of knee joint MRI in ACS group was shortened from 461 s to 245 s. There was no significant difference in SNR and CNR of bone marrow, anterior cruciate ligament and posterior cruciate ligament between the two groups (P>0.05). And there was no significant difference in the subjective scores of the two groups (P>0.05). In addition, the subjective measurement result of the two radiologists was in good agreement (Kappa_acs=0.79,Kappa_control=0.72). Conclusion: AI-assisted compressed sensing can significantly shorten the acquisition time without influencing the image quality, which has great potential in knee joint MRI. This technology can provide new ideas for rapid magnetic resonance imaging.

Objective: To construct a strong inducible expression system based on T7-like RNA polymerase MmP1 in Escherichia coli Nissle 1917 (EcN) to achieve efficient expression of the target protein. Methods: Constructing different inducible expression systems in EcN for “dose-response” characterization to screen out the best-performing induced system with low leakage and high induction expression level. Optimizing the expression output and dynamic range of the T7-like system by integrating the T7-like RNA polymerase (MmP1) expression on the chromosome of EcN, namely strain LM01, thereby constructing promoter mutation library of P_MmP1 for tunable induced expression output. T7-like induced system based on MmP1 was used to control the expression of superoxide dismutase (SOD) of high yield, giving strong evidence for protein expression of T7-like system in EcN. Results: Several inducible systems were successfully constructed and characterized in EcN. Of which, the T7-like induction system based on MmP1 RNA polymerase displayed the best-performing saturated induction output with maximum dynamic range reaching up to 909-fold. After the MmP1 polymerase expression module was integrated into the genome of EcN, the saturated induction level was increased by 66.8%. The construction of P_MmP1 promoter library provides a wide range of inducible expression output (dynamic range), spanning 65 to 1 097 fold. The T7-like (MmP1) system demonstrated the highest soluble SOD protein production yield of 435.7 mg/L, which was 4.02-fold higher than vanillic acid induction system. In addition, the maximum enzymatic activity of SOD achieves 391.3 U/mL. Conclusion: T7-like (MmP1) induction system can be successfully constructed in EcN with high saturated expression level, low basal leakiness, as well as controllable dynamic range of tunable gene expression control for efficient protein synthesis in the coming future.

Currently, there is limited systematic research on postoperative rehabilitation protocols for Maisonneuve fractures regarding functional recovery and long-term athletic performance maintenance in elite athletes. This article presented a case report on personalized and sport-specific rehabilitation protocol after Maisonneuve fracture surgery of professional basketball player, integrating progressive weight-bearing training, isokinetic strength enhancement, and neuromuscular control exercises. By 32 weeks postoperatively, the patient's ankle ROM and muscle strength had recovered to over 90% of the contralateral side, meanwhile the visual analogue scale (VAS) pain score had decreased from 7 to 1. Through a 10-year follow-up, the athlete had maintained professional competitive performance, with the American orthopaedic foot & ankle society (AOFAS) score consistently at 100 points, while without any complications such as post-traumatic osteoarthritis observed. This case illustrated that a staged rehabilitation protocol based on sport biomechanical characteristics could effectively promote functional reconstruction in athletes. Furthermore, the long-term safety and athletic-performance outcomes observed in this protocol might provide valuable insights for similar clinical cases.

Objective: The present study aimed to analyze the human papillomavirus (HPV) infection rate and subtype infection among 14 794 samples in Guangzhou, and to explore the association between HPV infection and ThinPrep Cytology Test (TCT). Furthermore, it evaluated the epidemiological features of HPV infection in Guangzhou, Guangdong Province, provided epidemiological insights into HPV infection among men, and assessed the significance of combined HPV and TCT testing in cervical cancer screening for women and in devising vaccination strategies against HPV. Methods: Sample data from 14,794 individuals who underwent genotyping for 37 HPV subtypes at the Second Affiliated Hospital of Guangzhou University of Chinese Medicine between January 2023 and December 2023 were collected. Concurrent analysis of TCT screening results was conducted to statistically examine the HPV infection characteristics across different ages, genders and seasons, as well as the relationship between single HPV infection, multiple HPV infection and TCT results. Results: The overall HPV infection rate stood at 29.42%. The most prevalent HPV subtype infections were HPV52 (21.55%), HPV58 (10.00%), HPV16 (9.17%), HPV51 (8.37%), HPV39 (7.56%), HPV61 (7.34%), and HPV53 (7.01%). The infection rate was 29.03% among women and 43.25% among men. The HPV infection rate exhibited a close correlation with age distribution, with statistically significant variations noted in the overall infection rate, single infection rate, and multiple mixed infection rate across different age groups ( X²=144.641, 30.797; P<0.001). Seasonal variations in infection rates were also observed, with rates of 28.03% in spring, 28.58% in summer, 29.56% in autumn, and 32.26% in winter. Among single infections, the highest infection rate of high-risk subtypes occurred in summer (43.53%), while among multiple infections, the higher infection rate was noted in autumn (35.02%). The TCT groups were predominantly characterized by single infections. Significant differences were discernible between single infections and multiple infections within each TCT group ( X²=90.497, P<0.001). Notably, TCT results also varied significantly among different age groups ( X²=32.871, P<0.001). Conclusion: The present study illuminated the epidemiological profile of HPV infection in the Guangzhou area, with an overall infection rate of 29.42%. HPV52, HPV58, and HPV16 emerged as the most frequent types. The overall HPV infection rate among men (43.25%) surpassed that among women (29.03%). The HPV infection rate showed a strong correlation with age distribution and was higher in summer and autumn compared to spring and winter. Additionally, this study identified significant differences in HPV infection rates among TCT groups and TCT results across different age groups, furnishing a scientific rationale for early HPV screening and presenting novel perspectives on cervical cancer prevention and treatment. In summary, this study contributes vital epidemiology data on HPV infection in Guangzhou, providing a scientific foundation for the formulation of targeted HPV prevention and control strategies and enhancement of cervical cancer diagnosis and treatment. It underscores the crucial role of male HPV infection in HPV prevention and control efforts.

Rapid advancements of 3D printing technology in the biomedical field have provided new solutions for vascular regeneration. This paper summarized applications of 3D printing in vascular regeneration, highlighting innovations in biomaterial design, vascular structure construction and cell carrier development. It details the characteristics of common vascular scaffold materials, the release systems of bioactive factors, and the establishment of simulation models. By analyzing the impacts of printing technology, biomaterials and active factors on vascular regeneration in practical applications, this paper discussed how to further utilize 3D printing technology to accurately build biocompatible vascular tissue models. Additionally, it addressed future directions including the selection of biocompatible materials, printing precision, cell survival rate, and maintenance of function. 3D printing technology has broad prospect in the field of vascular regeneration, but challenges in biocompatibility and functionality must be overcome for broad clinical application.

Objective: To evaluate the effects of simplified finger exercise on improvement of comfort degree and decrease related complications during transradial coronary procedures. Methods: This study randomized 302 patients who underwent a transradial coronary procedure in Huangshi central hospital during 2022 to one of the following 2 groups, (i) simplified finger exercise in addition to routine pre-procedure care (test group, 151 patients), (ii) routine pre-procedure care (control group, 151 patients). The achievement ratio of radial puncture, incidence of radial artery spasm and dissection (RAS, RAD), change of limb circumference, and painfulness of the limb were observed and analyzed between two groups. Results: Compared to the control group, test group had a higher achievement ratio of radial puncture, lower incidence of RAS and RAD, slighter tumidness of limb and patient felt less pain in test group. Conclusion: Simplified finger exercise has a positive effect on improvement of a patient's comfort degree and decrease related complications during transradial coronary procedures.

Objective: Based on Donabedian “structure-process-outcome” (SPO) three-dimensional quality evaluation model, this study adopts Delphi method and analytic hierarchy process construct the quality evaluation index system of nosocomial infection management in general hospitals. Methods: Through literature review and group discussion, the indexes of nosocomial infection management quality evaluation were initially drawn up, and 20 experts were selected by Delphi method for two rounds of correspondence consultation to determine the index system of nosocomial infection management quality evaluation, and the weights of each index were calculated by analytic hierarchy process. Results: The positive coefficient of the two rounds of expert correspondence was 100%. The expert authority coefficients were 0.869 and 0.877, Kendall's W were 0.156 and 0.219, respectively, and finally formed a 3-dimensional, 21-item nosocomial infection management quality evaluation index system. Conclusion: After two rounds of expert consultation, the indexes were modified and sorted out, and the results of the selected quality evaluation indexes of nosocomial infection management were reliable and the weights of each index were reasonable, which could provide an objective and quantifiable basis for nosocomial infection management.

Objective: To characterize the blood-borne occupational exposures among healthcare workers in a tertiary general hospital in Shenzhen from 2019 to 2023 and determine the influencing factors, aiming to provide insights for continuously improving the management of occupational exposures and strengthening the protective measures. Methods: An observational descriptive study was performed by a retrospective retrieval of five-year data of exposures to blood among healthcare workers in a tertiary general hospital in Shenzhen. The demographic distribution, exposure routes and exposure sources were descriptively statistically analyzed. Results: From 2019 to 2023, a total of 359 cases of blood-borne occupational exposures were reported. The annual incidence rate fluctuated, of which the highest was 3.59% in 2019 and the lowest was 1.53% in 2022. The exposure mainly occurred in the second half of the year. The majority of the affected individuals were female and nurses, with 79.39% of them having less than 5 years of work experience. 84.68% of occupational exposures were caused by sharp instrument injuries, mainly occurring during needle withdrawal and disposal (22.70%) and surgery (21.05%). The main exposure sites included ward (42.34%) and operation rooms (20.61%). Most of exposure sources were tested negative for blood-borne pathogens except for 131 cases. The top three pathogens were hepatitis B virus, Treponema pallidum and hepatitis C virus. Follow-up monitoring suggested that 77.78% of the exposed individuals completed the recommended preventive medication and no post-exposure infection occurred after exposure. Conclusion: The blood-borne occupational exposure reported in this hospital during 2019 and 2023 was mainly needle stick injuries in junior nurses, and the main pathogen exposed was hepatitis B virus. No post-exposure infection occurred. Nurses are high-risk group of occupational exposure, and the prevention of needle-stick injury calls for our great concern. Meanwhile, vaccination against pathogens from occupational exposure especially hepatitis B, should be paid great attention as an effective protective measure.