目的: 探讨在非小细胞肺癌(NSCLC)中SNORA56的基因性状及其临床意义.方法: 从癌症基因组图谱(TCGA) 数据库下载NSCLC癌组织及癌旁组织中SNORA56数据及临床信息资料,分析SNORA56表达水平与NSCLC临床病理学参数的相关性及预后的影响.收集广西医科大学第一附属医院的NSCLC患者标本,利用PCR技术检测30例NSCLC患者组织中SNORA56的表达,进一步验证SNORA56在NSCLC中的表达意义.结果: TCGA结果显示:SNORA56在癌组织中的表达量明显高于癌旁组织,具有统计学差异(P<0.001);SNORA56表达水平在有淋巴结转移的肺鳞状细胞癌(LUSC)癌组织中明显高于无淋巴结转移的LUSC癌组织(P<0.05);基因富集分析结果显示SNORA56可能通过细胞周期凋亡相关通路影响肺癌的发展.临床样本验证结果显示:PCR结果显示,与癌旁组织相比,LUSC与肺腺癌(LUAD)癌组织中SNORA56表达水平均明显升高;SNORA56表达水平在有淋巴结转移的LUSC癌组织中明显高于无淋巴结转移的LUSC癌组织(P<0.05);基因富集分析结果显示SNORA56可能通过细胞周期凋亡相关通路影响肺癌的发展.结论: SNORA56在LUSC及LUAD癌组织中的表达明显高于癌旁组织;SNORA56在有淋巴结转移的LUSC癌组织中的表达明显高于无淋巴结转移的LUSC癌组织;SNORA56可能通过细胞凋亡通路影响NSCLC发展.
Abstract
Objective: To investigate the gene character and clinical significance of SNORA56 in non-small cell lung cancer (NSCLC). Methods: The data and clinical information of SNORA56 in carcinoma and para-cancerous tissues of NSCLC were downloaded from the Cancer Genome Atlas (TCGA) database, and the correlation between SNORA56 expression level and the clinicopathological parameters of NSCLC and the influence on prognosis were analyzed. Samples of NSCLC patients from the first affiliated hospital of Guangxi medical university were collected, and the expression of SNORA56 in tissues of 30 patients with NSCLC was detected by PCR, so as to further verify the expression significance of SNORA56 in NSCLC. Results: TCGA data showed that the expression level of SNORA56 in carcinoma tissues was significantly higher than that in para-cancerous tissues, and the difference was statistically significant (P<0.001). The expression level of SNORA56 in lung squamous cell carcinomas (LUSC) with lymph node metastasis was significantly higher than that without lymph node metastasis (P<0.05). Gene enrichment analysis revealed that SNORA56 may influence the development of lung cancer through cell cycle apoptosis-related pathways. Verification results of clinical samples showed that compared with para-cancerous tissues, the expression level of SNORA56 in LUSC and lung adenocarcinoma (LUAD) was significantly increased. The expression level of SNORA56 in LUSC with lymph node metastasis was significantly higher than that without lymph node metastasis (P<0.05). Gene enrichment analysis showed that SNORA56 was likely to influence the development of lung cancer through apoptosis-related pathways in the cell cycle. Conclusion: The expression of SNORA56 in LUSC and LUAD was significantly higher than that in para-cancerous tissues. SNORA56 was significantly higher in LUSC with lymph node metastasis than that without lymph node metastasis. SNORA56 may influence the development of NSCLC through the apoptosis pathway.
关键词
癌症基因组图谱 /
非小细胞肺癌(NSCLC) /
SNORA56 /
临床样本
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Key words
TCGA /
non-small cell lung cancer (NSCLC) /
SNORA56 /
clinical samples
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参考文献
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脚注
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基金
国家自然科学基金项目(81660488)
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