基于单剪试验模型，采用割线形式卸载的双线性剪切－滑移本构关系对ＦＲＰ侧面黏贴抗剪加固混凝土梁的界面破坏过程进行了研究，详细分析了中间裂缝的扩展过程．首先依据裂缝两侧不同的黏结长度分布对裂缝进行详细分类，在此基础上绘制了详细的界面破坏流程图，推导得出了每一类裂缝每一阶段裂缝处的正应力和裂缝宽度的解析表达式，由此得到了界面破坏过程中裂缝处的正应力与裂缝宽度关系曲线，通过构造的有限元模型对文中的算例进行了数值模拟，验证了理论推导的准确性和可靠性．

目的：观察针刺治疗抑郁症的临床疗效。方法：将７２例抑郁症患者随机分为治疗组和对照组，每组３６例。治疗组采用针刺治疗，对照组给予盐酸氟西汀治疗，治疗前后采用汉密顿抑郁量表（ＨＡＭＤ）进行评分以观察疗效。结果：经４个疗程治疗后，治疗组临床疗效和ＨＡＭＤ评分与对照组相比，差异具有统计学意义（Ｐ＜００５）。结论：针刺治疗抑郁症疗效优于西药盐酸氟西汀，且是一种治疗抑郁症的有效方法

Objective: To investigate the prognostic factors after laparoscopic ultrasound-guided microwave ablation treatment for hepatocellular carcinoma. Methods: A total of 81 patients treated by laparoscopic ultrasound guided microwave ablation were retrospectively analyzed in our study. Among them, 55 cases were primary cancer, including 37 cases of stage Ⅰ and Ⅱ, 18 cases of stage Ⅲ and Ⅳ of the Union for International Cancer Control (UICC), and 26 cases of recurrent cancer. The follow-up period ranged from 3 to 72 months. Number of 24 possible factors related to postoperative survival were analyzed to explore the correlation with survival. Kaplan-Meier model and Log-rank test were used for univariate analysis, and COX proportional hazard model was used for multivariate analysis. Results: The 1-, 2-, 3-, and 5- years cumulative overall survival rates of 81 patients were 79.1%, 63.5%, 52.3%, 43.1%, respectively, and the 1- 2- 3-, and 5-year progression-free survival rates were 50.7%, 37.2%, 24.0%, 12%, respectively. Of the 24 potential factors 14 factors were statistically significant, including basic diseases, types of liver cancer, UICC stage, degree of tumor differentiation, histological type, Child-Pugh grade, number of lesions, multi-segment of lesions, tumor size, preoperative alpha fetoprotein (AFP) level, postoperative AFP level, ablation status, tumor progression, tumor progression time. The 1-, 2-, 3-, and 5-year survival rates of the UICC stage Ⅰ and Ⅱ group were 94.1%, 90.6%, 78.8%, 67.1%, respectively and the 1- and 2-year survival rates of stage Ⅲ and Ⅳ group were 60.0%, 28% respectively, and there were statistically significant differences between stage Ⅰ and Ⅱ and stage Ⅲ and Ⅳ groups ( x²=18.349, P=0.000). the 1-, 2-, 3-, and 5-year survival rates for recurrent cancer were 84.0%, 49.0%, 32.7%, 0.0% respectively, and there were statistically significant differences between primary and recurrent cancer ( x²=4.193, P=0.041) while there was no statistical difference between recurrent group and stage Ⅲ and Ⅳ group. Multivariate analysis showed that histological type, Child-Pugh grade, multi-segment lesions, postoperative AFP level, tumor progression time were independent factors for overall survival. Conclusion: Factors influencing the prognosis of patients with primary liver cancer treated by laparoscopic ultrasound-guided microwave ablation include (1).Tumor factors, including histological type, multi-segment lesions; (2)Patient and other factors include Child-Pugh grade, postoperative AFP level and tumor progression time.

  细胞自噬是维持细胞内环境自稳的一种自我保护机制，是由溶酶体介导的降解细胞内受损的蛋白质或者细胞器的代谢过程。细胞自噬与炎症反应密切相关，模式识别受体Toll样受体的激活能够诱导细胞自噬的发生，而细胞自噬又对炎症反应有调控作用。同时细胞自噬与抗感染免疫密切相关，而细胞自噬的缺损是诱发炎症反应和炎症性疾病的一个重要因素。本文简要综述细胞自噬与炎症反应的相互作用，为研究细胞自噬调控炎症反应的机制提供参考。

With the development of material science,it is necessary to build effective methods to understand nano-particles.Transmission electron microscope(TEM) can be used to measure size and morphology of nano-particles.Combined with selected area electron diffraction(SAED),it could further identify the crystal phases and structure of samples,thus to increase the accuracy and reliability in the analysis of samples.In the paper the advantages of combination of TEM and SAED are presented.The progress of application o...

Long non-coding RNAs(lncRNAs) are RNA transcripts longer than 200 bp without no protein coding capacity.lncRNAs regulate the expression of genes at the epigenetic,transcriptional and post-transcriptional levels.They are widely involved in biological and pathological changes.lncRNAs play a key role in cancer development.

Objective: To monitor the treatment of early changes in tongue subcutaneous transplanted tumor in nude mice by silencing miRNA-221, using the IVIM-DWI technique and to investigate the correlation between the early pathological and physiological changes in tumor and the IVIM parameters after silencing miRNA-221 treatment. Methods: Twelve nude mice were randomly divided into examination group and control group.The experimental group mice were subcutaneously injected with lentiviral transfected UM1 cells which were stably low expression with miR-221, and the control group mice were subcutaneously injected with lentiviral empty transfected UM1 cells. All of the nude mice were received the intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI) at 7 d and 21 d. The pure water molecular diffusion coefficient (D),perfusion-related diffusion coefficient (D^* value) and perfusion fraction (f value) values were measured dynamically. And all of them were sacrificed at 21 d, and the tumor samples were treated with the routine HE and CD31 immunohistochemical staining. Results: After subcutaneous inoculation of UM1 cells by silencing miR-221 in nude mice, the D value of the experimental group was significantly higher than that of the control group on 7 d and 21 d, with statistically significant difference(P<0.01). While the D^* and f values of the experimental group were both significantly lower than that of the control group on 7 d and 21 d, with statistically significant difference(P<0.05). Conclusion: Among the IVIM-DWI parameters, D, D^* and f can dynamically monitor and quantitatively evaluate the early effect of silencing miR-221 on tongue subcutaneous transplanted tumor in nude mice. And the changes in D, D^* and f values are closely related to the pathology and CD34.

Objective: To explore the correlation between serum leukemia inhibitor factor (LIF), heparin-binding epidermal-growth-actor-like growth factor (HB-EGF) and pregnancy outcome after frozen thawed embryo transfer and to analyze their predictive value in pregnancy success. Methods: 92 cycles of patients planning to accept frozen thawed embryo transfer from March 2019 to December 2019 were selected. The follow-up was divided into pregnancy group (32 cycles) and non pregnancy group (60 cycles) according to the pregnancy outcome. The factors possible to affect pregnancy outcome were compared in the patients of two groups, including age, infertility duration, endometrial morphology and endometrial thickness on the day of transfer. The levels of LIF and HB-EGF were also compared between the two groups. The risk factors of pregnancy failure were determined by logistic regression analysis. By drawing the receiver operating characteristic curve (ROC) of subjects, the predictive value of serum LIF and HB-EGF levels on pregnancy success was analyzed..Results: There were significant differences in endometrial morphology and endometrial thickness between pregnant group and non pregnant group (P<0.05). The serum LIF level [(24.45±2.91) pg/mL vs. (19.32±2.04) pg/mL] and HB-EGF level [(3 320.59±300.74) pg/mL vs. (2 430.55±261.37) pg/mL] of pregnancy group was higher than that of non pregnancy group (P<0.05) on the day of transplanation. The logistic regression analysis showed that endometrial morphology, endometrial thickness on the day of transplantation, and serum LIF and HB-EGF levels were all risk factors of pregnancy failure after frozen thawed embryo transfer (OR=3.074, 2.563, 4.437, 3.881, P<0.05). The ROC analysis indicated that the sensitivity, specificity, accuracy and AUC of serum LIF in predicting pregnancy success after frozen thawed embryo transfer were 84.38%, 76.67%, 79.35% and 0.760, respectively, and the sensitivity, specificity, accuracy and AUC of serum HB-EGF were 78.13%, 73.33%, 75.00% and 0.722, respectively. Conclusion: Serum LIF and HB-EGF level are closely related to the outcome of pregnancy after frozen thawed embryo transfer, and they have a high predictive value for the success of pregnancy after transfer.

Objective: Known about the cognition and real demand of medical humanities of clinical professional degree students, providing theoretical basis and strategy to practice bio-psycho-social medical model. Methods: To investigate the cognition and real demand of the medical humanities for full-time clinical professional degree students by questionnaire..Results: Clinical professional degree students have certain cognitive of the medical humanities, 72.4% students can realize that the core idea of medical humanities is love, awe and care for life; their demand for the medical humanities knowledge are relatively large, 91.5% of the students believe their medical humanities level needs to be further improved; The demand analysis of medical humanities courses and course teaching methods found that: clinical professional degree students have a large demand for Doctor-patient Communication (28.7%), Medical Psychology (21.8%), Medical Ethics (16.5%), and Medical Law (13.4%), compared with traditional classroom infusion teaching (2.1%), they prefer teacher-student interactive teaching (33.0%), heuristic teaching (27.5%), individual clinical guidance (19.0%) or group discussion (18.4%). Conclusion: Medical educational institutes should take effective methods form multiple aspects and levels to establish and improve the education of medical humanities, improving the humanistic quality of clinical professional degree students.

Objective: To establish a new in vitro hemolysis detection technology for detecting low level blood group antibodies in serum. Methods: A total of 33 samples of isolated mononuclear cells were resuspended with autologous plasma, and reacted with the hemolysis detection system in this study. The Hb colloidal gold strip was used for qualitative detection, and then the results were compared and analyzed with the results of indirect anti-human globulin detection by saline method and gel card method. Statistical significance was assessed using Mann-Whitney Test in SPSS 19.0. Results: Compared with the indirect anti human globulin test of brine and gel card, the new hemolysis technology had higher detection rate of irregular antibodies (IgG/IgM) than brine and gel method. The detection rate of irregular antibodies of different kinds (RH, MNSs, Duffy, Kidd, Lewis and jmh system and autoantibodies) was higher than that of other two methods (P<0.05). Conclusion: The new in vitro hemolysis detection technology can improve the sensitivity of detection, detect various irregular antibodies effectively, avoid the hemolytic transfusion reaction of patients with repeated blood transfusion, and ensure the safety of blood transfusion.

Objective: To explore the clinical characteristics and prognostic factors of patients with hematological malignancies associated hypercalcemia. Methods: The data of 153 patients with hematological malignancies associated hypercalcemia were analyzed retrospectively. The correlation between clinical characteristics and serum calcium level was analyzed by correlation analysis and multiple linear regression. The survival curve of Kaplan-Meier was drawn, and the prognostic factors were analyzed by log-rank test and Cox proportional risk model..Results: The incidence rate of hematological malignancies associated hypercalcemia was 3.9% before correction of serum calcium, while 13.1% after correction, and the median survival time after diagnosis of hypercalcemia was 13 months. Correlation analysis showed that serum calcium level was correlated with serum albumin, serum globulin, creatinine and platelet. Multiple linear regression showed that serum albumin, serum globulin and creatinine could significantly affect serum calcium level. Single factor analysis showed that serum calcium, hemoglobin and creatinine were the important factors affecting the prognosis. Multivariate Cox regression analysis showed that the concentration of serum calcium ≥2.9 mmol/L and the mass concentration of hemoglobin <90 g/L were the independent risk factors affecting the prognosis. The median survival time of patients scored 0, 1 and 2 in the prognostic factor scoring system was 20, 18 and 4 months, respectively. Conclusion: Corrected calcium can more accurately reflect the incidence rate of hematological malignancies associated hypercalcemia. Most of the patients with hematological malignancies associated hypercalcemia had late stage and short median survival time. Serum albumin, serum globulin and creatinine can significantly affect the level of serum calcium. Hypercalcemia (the concentration of serum calcium ≥2.9 mmol/L) and moderate or above anemia (the mass concentration of hemoglobin <90 g/L) can independently affect the prognosis of patients with hematological malignancies associated hypercalcemia, and the more independent factors, the worse the prognosis.

Objective: To observe the clinical responses to two plasma concentrations of tacrolimus in treatment of idiopathic membranous nephropathy (IMN) with nephrotic syndrome, including both efficacy and complication, thus to provide evidence for clinical rational application of tacrolimus. Methods: A total of 66 patients diagnosed with nephrotic syndrome and as IMN through renal puncture biopsy in our hospital was selected. The clinical data and tacrolimus concentration of the patients were collected. The level of serum albumin, urinary protein, cholesterol and serum creatinine (Scr), the remission rate and adverse reactions of patients were observed for comparative analysis..Results: Serum albumin in the two groups was significantly higher than that before treatment, and the difference was not statistically significant (P>0.05). The quantitative level of proteinuria of 24h was significantly lower than that before treatment (P<0.05), but there was no statistical difference in the quantitative level of urinary protein at the same time between the two groups (P>0.05). There was no significant change in Scr level in the two groups during treatment (P>0.05). The cholesterol level of the two groups was significantly reduced (P<0.05), but there was no statistical difference in the cholesterol level of the two groups at the same time (P>0.05). In terms of remission rate, the high concentration group was higher than the low concentration group, but there was no statistical difference between the two groups (P>0.05). In terms of adverse drug reactions, the number of cases of adverse drug reactions was higher in the group with high blood drug concentration than in the group with low blood drug concentration, but the difference between the two groups was not statistically significant.Conclusion: Tacrolimus had good remission rate at both low and high concentration and the incidence of adverse reactions was similar between the two groups. Tacrolimus can achieve clinical efficacy at low concentration.

Objective: To investigate the risk factors of intracranial hemorrhage transformation after mechanical thrombectomy in patients with acute cerebral infarction and to put forward some preventive suggestions. Methods: In shenzhen people's hospital, 45 cases of acute cerebral infarction after mechanical thrombectomy were recruited continously as participants. After collected the baseline data, according to the results of the review of head CT or MRI, patients were divided into HT and non HT groups to analyze the data of two groups of patients with baseline results, using Logistic regression was conducted to analyze the risk factors of intracranial HT.Results: The proportion of atrial fibrillation, ORT time, preoperative NIHSS scores, precirculation ratio of thrombectomy site, and postoperative systolic blood pressure in the HT group were significantly higher than those in the non-ht group, with statistical significant differences (P<0.05), P value were 0.026, 0.012, 0.041, 0.020, 0.038 respectively. Logistic regression analysis found that atrial fibrillation, ORT and preoperative NIHSS scores were independent risk factors for intracranial HT after thrombectomy in patients of acute cerebral infarction.Conclusion: Atrial fibrillation, prolonged ORT and higher preoperative NIHSS scores increase the risk of intracranial HT after mechanical thrombectomy in patients of acute cerebral infarction.

Objective: To assess the value of pulmonary arterial systolic pressure in predicting ischemic stroke event in patients with non-valvular atrial fibrillation (NVAF). Methods: A total of 198 hospitalized NVAF patients were included, and divided in stroke group (n=39) and non-stroke group (n=159). Risk factors were screened by statistical analysis, and their value in predicting ischemic stroke event was further evaluated. Results: The percentage of patients with high pulmonary atrial pressure (hPAP) was significantly higher in stroke group (61.5%) than in non-stroke group (26.4%, P<0.01). The results of unconditional logistic regression analysis showed that hPAP was an independent risk factor for ischemic stroke event in patients with NVAF (OR=4.072, P<0.01). ROC curve analysis showed that AUC of CHA2DS2-VASc-hPAP score was larger than that of CHA2DS2-VASc score, indicating that the predictive value of CHA2DS2-VASc-hPAP score was significantly better than CHA2DS2-VASc score.Conclusion: High pulmonary atrial systolic pressure (≥30 mmHg) is closely related to ischemic stroke in NVAF patients. The CHA2DS2-VASc-hPAP score might perform better in predicting ischemic stroke compared with the CHA2DS2-VASc score.

Objective: In order to understand the ecological process of alpine soil more comprehensively, the changes of soil microbe under exogenous carbon input in winter were analyzed. Methods: The alpine meadow in the east of Qinghai Tibet Plateau was taken as the research object and the response mechanism of alpine soil microbe to short-term exogenous carbon input in winter was studied..Results: The short-term exogenous carbon input significantly increased the number of microorganisms, especially fungi, which reached 7.23~22.27 times of the control. Short-term exogenous carbon input significantly increased the activities of soil cellulase and sucrase. The cellulase activity increased by 59.13%~62.33% and sucrase activity by 38.63%~169.23% compared with the control. It indicated that the carbon limitation of alpine soil in eastern Qinghai Tibet Plateau in winter was reduced or eliminated with the input of exogenous carbon. The freezing and thawing events that must be experienced in the alpine region in winter also have significant effects on soil microorganisms. The results showed that the soil invertase activity was significantly decreased by the large-scale freezing and thawing, while the soil fungal number, catalase and cellulase activities were significantly increased by the freezing and thawing events with small temperature changes. Conclusion: Moderate and mild freezing and thawing in winter in alpine regions in winter may be beneficial to microorganisms, and it is helpful to further study the process of soil nutrient transformation and plant nutrient supply.

Objective: The inhibitory effects of ICT on cisplatin-resistant ovarian cancer cell lines with different p53-type were studied, hoping to make contribution to the development of therapy for drug-resistant and recurrent ovarian cancer. Methods: Using thethymidine (TdR) double-block method to establish cell cycle synchronization model, the cycle arrest effect of ICT on synchronized cisplatin-resistant ovarian cancer cells with different p53 types were detected by flow cytometry, Western blot was used to detect the expression of CyclinE, CyclinB1, p53 and CHK1 protein after treated with ICT(20 μmol/L) for different time.Results: Cell cycle began to be arrested after 4 h treated by ICT (20 μmol/L) and the blocking effect was time depended. C13^* (p53-wild) cell line was arrested in G1 phase (P<0.05). A2780cp (p53-mutation) and SKOV3 (p53-null) cell lines were arrested in G2 phase (P<0.05). After treated with ICT (20 μmol/L), the expression of CyclinE in C13^* (p53-wild) cell line was down-regulated (P<0.05) without changes in CyclinB1. The expression of CyclinB1 in A2780cp (p53-mutation) and SKOV3 (p53-null) cell lines were down-regulated (P<0.05) without changes in CyclinE. The expression of p53 was up-regulated in C13^* (p53-wild) cell line, unchangeable in A2780cp (p53-mutation) cell line and non-expressed in SKOV3 (p53-null) cell line. The expression of CHK1 protein in three cell lines was significantly increased (P<0.05).Conclusion: -Icariin had time-dependent cell cycle arrest effect on cisplatin resistant ovarian cancer cells with different p53 subtypes. ICT arrested cell cycle in G1 phase by degrading the Cyclin-E through p53 in C13^* (p53-wild) cell line, and arrested cell cycle in G2 phase by degrading the Cyclin-B1 through CHK1 in A2780cp (p53-mutation) and SKOV3 (p53-null) cell lines. The effects was time depended.

Objective: To study the effects and mechanism of PM_2.5 on the proliferation and migration of human hepatic stellate cells. Methods: Human hepatic stellate cells LX-2 were cultured in vitro and divided into control group, PM_2.5 treatmented group (the final concentration of PM_2.5 was 5 μg/mL, 10 μg/mL and 20 g/mL, respectivley), PM_2.5 with SB525334 treatmented group. The proliferation of LX-2 cells was detected using CCK-8 assay. The cell migration was measured by Transwell assay and cell scratch test. The relative mRNA and protein expression level of TGF-β1 was evaluated by RT-PCR and ELISA. Results: The proliferation ability and migration ability of LX-2 were significantly upregulated in LX-2 cells treated with PM_2.5 (P<0.05, PM_2.5 treated group vs. control group). Also, the expression of TGF-β1 was regulated by PM_2.5. Our results showed that the mRNA and protein expression of TGF-β1 were both obviously increased with PM_2.5 treatment (P<0.05, PM_2.5 treated group vs. control group). Furthermore, inhibition of TGF-β1 signaling pathway with SB525334, a TGF-β1 receptor inhibitor, downregulated the the proliferation and migration of PM_2.5 -treated LX-2 cells (P<0.05, PM_2.5 plus SB525334 treated group vs. PM_2.5 treatment group).Conclusion: Treatment with PM_2.5 actives human hepatic stellate cells LX-2 by promoting cell proliferation and migration, and TGF-β1 signaling pathway maybe involved in this process.

Objective: To establish the rat model of oligoasthenospermia with liver-depression and kidney-deficiency and discuss the related evaluation indexes. Methods: Forty SPF SD male rats were randomly divided into blank group, model group, liver-depression group and kidney-deficiency group. In model group, rats were intragastrically administered 50 mg of adenine per day per 100 g of body weight for 14 days and chronic restraint stress was given for 21 days at the same time. In liver-depression group, chronic restraint stress was given for 21 days. In kidney-deficiency group, rats were intragastrically administered 50 mg of adenine per day per 100 g of body weight for 14 days and then kept for 7 days of normal feeding. After modeling, the general conditions and behavioral changes of the rats were observed, body weight and reproductive organ weight were weighed, and sperm quality was analyzed. The mass concentration of serum cAMP and cGMP (ρ_cAMP, ρ_cGMP)were detected by radioimmunoassay, and testicular sections were observed by light microscopy..Results: There were significant differences in the behavior test and sperm quality between the liver depression group and the blank group (P<0.01), indicating that the model of oligoasthenospermia of liver depression was successfully constructed. The ρ_cAMP, ρ_cGMP and ρ_cAMP/ρ_cGMP in serum and sperm quality were significantly different between the kidney deficiency group and the blank group (P<0.01), indicating that the oligoasthenospermia model of kidney deficiency was successfully constructed. There were significant differences in the behavior test and sperm quality in the model group (P<0.01) compared with the liver depression group, and there were significant differences in the ρ_cAMP, ρ_cGMP and ρ_cAMP/ρ_cGMP and sperm quality between the model group and the kidney deficiency group (P<0.01), which preliminarily indicated that the rat model of oligoasthenospermia with liver depression and kidney deficiency was successfully constructed. Conclusion: Adenine gavage combined with chronic restraint stress stimulation could establish the rat model of oligoasthenospermia with liver-depression and kidney-deficiency.

目的：为了了解阳春砂磷酸甲羟戊酸激酶（ＰＭＫ）的功能与表达特性，根据阳春砂的转录组注释设计引物，ＰＣＲ克隆阳春砂甲羟戊酸途径中的关键酶磷酸甲羟戊酸激酶基因的编码区ｃＤＮＡ，使用生物信息学方法对其编码的蛋白进行相似性检索及同源序列比对，构建进化树，预测二级及三级结构并分析其功能，探索其各器官中的表达差异．结果：获得了全长１５３９ｂｐ的编码阳春砂ＰＭＫ的ｃＤＮＡ序列，命名为ＡｖＰＭＫ（ＧｅｎＢａｎｋ登录号：ＫＦ５６９９０２），编码由５１２个氨基酸组成的磷酸甲羟戊酸激酶，该蛋白含有ＧＨＭＰ激酶超家族特异的Ｎ－保守区域、Ｃ－保守区域和ＡＴＰ结合位点ＧｌｙＸＧｌｙＸＸＡｌａ．ＡｖＰＭＫ与其他植物的ＰＭＫ氨基酸相似性达６１％ ～６９％，进化树结果显示其与二穗短柄草（Ｂｒａｃｈｙｐｏｄｉｕｍｄｉｓｔａｃｈｙｏｎ）等单子叶植物具有较近的亲缘关系．ＡｖＰＭＫ蛋白包含１４个α螺旋（Ａｌｐｈａｈｅｌｉｘ），占３７１％；１６个β链（Ｂｅｔａｓｔｒａｎｄ），占１５６％；３２个无规则卷曲结构，占４７３％，无跨膜结构域，其３级结构含有一个催化反应的口袋状结构域．实时荧光定量ＰＣＲ结果显示ＡｖＰＭＫ基因在叶中表达量较高，茎、根中表达量相对较低．结论：首次从阳春砂仁中扩增磷酸甲羟戊酸激酶的基因片段，为分析其基因表达特性及其在砂仁萜类生物合成中的功能奠定基础．

Objective: To explore the inhibitory effect and molecular mechanism of azole antifungal agents on lung squamous cell carcinoma H520 cells and their effect on cisplatin efficacy. Methods: MTT method was used to detect the effects of different azole antifungal agents on the activity of lung squamous cell carcinoma H520 cells, and the best azole antifungal was screened out. Flow cytometry was used to analyze the effect of the best azole antifungal agent on apoptosis and reactive oxygen species (ROS) production of lung squamous cell carcinoma H520 cells. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the effect of the best azole antifungal agent on the expression of NADPH oxidase-4 (NOX4) mRNA in lung squamous cell carcinoma H520 cells. The protein expression of p-Akt, Akt and NOX4 were examined by western blotting. The effects of the best antifungal agent and its combination with cisplatin were verified by nude mice in vivo tumor models. Immunohistochemical assay was used to detect the expression of p-Akt and NOX4 protein in tumor tissues..Results: Fourteen azole antifungal agents inhibited the viability of lung squamous cell carcinoma H520 cells, and the half-inhibitory concentration of miconazole was the lowest, so it was selected as the best azole antifungal agent. Miconazole could reduce the generation of ROS and induce apoptosis in lung squamous cell carcinoma H520 cells. Miconazole could inhibit the expression of p-Akt and NOX4, and after the combination of miconazole and PI3K/Akt pathway inhibitor LY294002, the expression of NOX4 protein was not significantly different from that of the group administered with LY294002 alone. Miconazole could reduce the promoting effect of cisplatin on the expression of p-Akt in lung squamous cell carcinoma H520 cells and increase the sensitivity of cisplatin. In vivo experiments in tumor-bearing nude mice, miconazole could inhibit the growth of tumors. The immunohistochemical results showed that miconazole could reduce the protein expression of NOX4 and p-Akt in nude mouse tumors. Conclusion: Miconazole may inhibit the expression of NOX4 mediated by PI3K/Akt, induce apoptosis of lung squamous cell carcinoma H520 cells and enhance the inhibitory effect of cisplatin on lung squamous cell carcinoma H520 cells.

Objective: To explore the expression and clinical prognosis of monoglyceride lipase (MGLL) in prostate cancer. Methods: Using GEPIA (gene expression profiling interactive analysis), Oncomine and THPA (The human protein atlas) bioinformatical tools to analyze the mRNA and protein expression of MGLL in prostate cancer from public databases TCGA and GEO. Survival rate was analyzed based on prostate cancer patients from the TCGA database and the co-expression of key proteins between the MGLL and fibroblast growth factor FGF/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway was analyzed. The expression of MGLL in prostate cancer cell lines was observed by western blot; the effect of overexpression of MGLL on the migration and invasion of prostate cancer cells was observed by Transwell assay..Results: Six prostate cancer research cohorts were retrieved from the Oncomine database. The GEPIA tool analyzed 492 prostate cancer samples and 52 prostate control samples. The results showed that in Oncomine's multiple prostate cancer cohort studies, compared with control prostate tissues, MGLL mRNA was significantly decreased in prostate cancer tissues (P<0.021). The TCGA cohort analyzed by the GEPIA tool also showed that the mRNA level of MGLL in prostate cancer was also significantly reduced (P<0.01). The THPA tool shows that MGLL protein was highly expressed in normal prostate tissues and lowly expressed in prostate cancer tissues. The trend of protein level was consistent with mRNA level. GEPIA survival analysis showed that the expression level of MGLL was closely related to the overall survival (OS) and disease-free survival (DFS) of prostate cancer patients, and the prognosis of patients with low MGLL expression was poor (P=0.018; P=0.034). Furthermore, protein co-expression analysis showed that MGLL and FGF receptors FGFR1, PIK3CA, AKT2, GSK-3B and CRMP2 were positively correlated in prostate cancer (R=0.6, P=0; R=0.36, P=0; R=0.22, P<0.001; R=0.36, P=0; R=0.5, P=0). Western blot results indicated that MGLL was lowly expressed in multiple prostate cancer cell lines, and overexpression of MGLL inhibited the migration and invasion ability of prostate cancer cell lines (P<0.05). Conclusion: MGLL is lowly expressed in prostate cancer tissues and is related to the prognosis of cancer patients. The mechanism may be related to the PI3K/AKT/GSK-3B signaling pathway.

Objective: To explore the targets and related signaling pathways of Andrographis paniculata for Intrahepatic cholestasis of pregnancy using network pharmacology, and animal experiments are applied to further clarify its mechanism of action. Methods: TCMSP database was used to screen the active chemical components of Andrographis paniculata, and varieties of systematic biological databases were integrated to construct the “active component-disease-target” network relationship, and the common PPI network genes were functionally enriched.The active ingredients were verified by animal experiments.Results: Andrographis paniculata contains 24 active ingredients the active ingredient related to Intrahepatic cholestasis of pregnancy 30 potential target proteins interact, and which contains JUN, ESR1, IL6, NOS2, DPP4, CCL2, NR3C2, KDR, CKD2, CCNA2, CDKN1A, PPARG, RELA and CASP9, etc. The experimental results showed that the blood biochemical indexes (BBI) showed that the ICP model was successful established, and the BBI levels in the high and medium dose group had significant decreased when compared with the model group (P<0.05), while there were none difference of BBI were found between the low dose group and the model group. The number of fetal in the high dose group was significantly higher than that in the other groups, and there was no significant difference between the low-dose group and the model group. Conclusion: Andrographolide can normalize the BBI in ICP rats, and has a certain restorative effect on fetal rats number and qulity.

  利用蔡司Ｕｌｔｒａ５５场发射扫描电镜观察原子力显微镜探针和聚苯乙烯微球在不同加速电压下的二次电子图像和能量选择式背散射电子图像．结果发现：在合适的低加速电压下，样品可以直接观察，不需喷金处理，而且二次电子图像所呈现的样品表面细节更丰富，更清晰；能量选择式背散射电子图像样品成分衬度明显，可以有效消除荷电现象和各种假象．

　以吡啶为溶剂，１１０℃加热６ｈ的反应条件下，芳香伯胺与丁二酸酐反应，主要生成Ｎ-芳香取代丁二酰亚胺，最高收率７３.２%；相反，脂肪伯胺与丁二酸酐反应，主要产物为Ｎ-取代丁二酸单酰胺，收率７２.５% ～８１.７%．探讨了结构－反应活性关系，并提出可能的反应机理．

针对目前协同过滤方法存在的数据稀疏性、冷启动以及未能有效利用用户社交网络信息提高推荐质量等问题，提出一种融合用户社交网络信息的协同过滤方法，该方法以矩阵分解推荐模型为核心，可综合集成目标用户个人偏好以及社交网络中的关系用户偏好特征信息做出推荐．通过设计相应的推荐方法，并基于梯度下降法对用户以及商品特征矩阵的求解进行了优化运算．相关实验结果表明融合社交网络信息可在一定程度上提高协同过滤的推荐准确度以及缓解数据稀疏性、冷启动问题．

ＨＩＶ-１相关神经认知障碍（ＨＩＶ-１ａｓｓｏｃｉａｔｅｄｎｅｕｒｏｃｏｇｎｉｔｉｖｅｄｉｓｏｒｄｅｒ，ＨＡＮＤ）是艾滋病患者中枢神经系统
（ｃｅｎｔｒａｌｎｅｒｖｏｕｓｓｙｓｔｅｍ，ＣＮＳ）的常见并发症，其患病率高达４０％ ～６０％，是４０岁以下成人痴呆的最主要原因之一；患者从确诊到死亡的平均时间约为４～６个月，且其发病机理尚待研究．已有的研究表明，ＨＩＶ-１不感染神经元，但在ＨＡＮＤ的发病机制中，神经胶质细胞的过度活化和由此而引发的神经炎症起着重要的作用．本文就神经胶质细胞在ＨＡＮＤ的发生发展中的作用进展进行综述．

With the process of growth and aging,human s red bone marrow will turn into yellow gradually,which is also known as bone marrow fatting.Its essence is the increased number of adipocytes in marrow.Many studies have showed that bone marrow adipocytes could regulate negatively the hematopoietic microenvironment,which inhibited the hematopoiesis and the embedding of hemopoietic stem cell in transplantation.Thus,further study of the mechanism of adipogenesis have significant influence on improving the hematopoie...

结合压电陶瓷变压器的特点，介绍了压电陶瓷变压器的等效电路．为了使压电陶瓷变压器获得良好的工作性能，必须使其工作在固有谐振频率并有良好的阻抗匹配，因此提出了将ＬＬＣＣ谐振电路应用在压电陶瓷变压器上的方法，并对该电路的特性进行了分析．分析表明，ＬＬＣＣ谐振电路能满足在压电陶瓷变压器的应用，并且根据其固有谐振频率的大小，可以推算出阻抗匹配电路的参数．

本实验以富含纤维素的废弃生物质甘蔗渣为原料，采用水热法合成了具有荧光性能的碳量子点．对碳量子点进行了表征，其粒径大小约为５～１０ｎｍ；在紫外灯照射下发蓝色荧光，对其荧光性能进行了研究，发现其具有多元激发、多元发射的性质．对其细胞毒性进行了研究，结果表明是一种生物相容性良好的标记材料．

多峰函数的寻优能力一直是衡量算法优越性的依据之一，许多传统的算法对其求解容易误将局部极值当做全局极值，针对这一问题，以Schaffer多峰函数为例，运用改进的果蝇优化算法（Improved Fruit Fly Optimization algorithm,IFFOA）对Schaffer函数进行寻优求解，同时对其他４个测试函数进行求解．通过matlab软件测试、分析了改进的果蝇算法寻优能力的影响因素，最后通过相同的参数，分别采用遗传算法（GA）和改进的果蝇算法进行对比，通过对比发现，改进的果蝇优化算法在收敛精度和收敛速度方面，均优于和声搜索算法（HS）算法，从而验证了IFFOA算法的优越性和有效性，可以将IFFOA算法应用于其他领域．