15 February 2019, Volume 40 Issue 1

    

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  Optimization of purification process of Lycopus lucidus polysaccharide with macroporous adsorption resins by response surface method

  LIU Fugang, LI Hanwei, ZHENG Qunjie, FENG Suxiang, LIU Jiong

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 1-9. https://doi.org/10.11778/j.jdxb.2019.01.001

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  Objective:To investigate the effect of 8 kinds of macroporous adsorption resins, including D3520, H103, HPD-100, HPD-700, AB-8, HPD722, S-8 and HPD-600, on purification of Lycopus lucidus polysaccharide and to optimize the process of purification by Box-Behnken design using the best macroporous adsorption resin. Methods: The influence of macroporous resin type, elution velocity, sample loading concentration, volume of eluent on the purification effect were evaluated with weighted comprehensive score of the rate of polysaccharide retention, decoloration and protein removal. Response surface model was established by Box-Behnken design to optimize the process parameters for purification of Lycopus lucidus polysaccharide with macroporous resin. Results: The optimized purification process and results for Lycopus lucidus polysaccharide with macroporous resin was as follows. Using HPD-100 macroporous resin for separation, the sample loading concentration was 0.03 g/mL, the elution velocity was 1.1 mL/min and the elution volume was 40 mL. Using this process, a polysaccharide retention rate of 69.21%, a decoloration rate of 60.24% and a protein removal rate of 75.67% was achieved. Conclusion:The developed purification process of Lycopus lucidus polysaccharides is stable and reliable. The purification process using HPD-100 macroporous adsorption resin generates good effect and is suitable for industrial production.
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  Si-Ni-San suppresses inflammatory responses via MAPK/NF-κB pathway in LPS-treated Raw 264.7 macrophage cells

  FAN Huijie, TAN Zhangbin, ZHAO Xiaoshan, LIANG Hongfeng, LIU Bin, ZHOU Yingchun

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 10-18. https://doi.org/10.11778/j.jdxb.2019.01.002

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  Objective:To observe the protective effect of Si-Ni-San (SNS) on the lipopolysaccharide (LPS)-induced inflammation in peritoneal Raw 264.7 macrophage cells and to explore its potential mechanism. Methods: Raw264.7 cells were cultured and divided into control group, model group and SNS groups of low, medium and high concentration. The morphological change of each group was observed under microscope. Elisa assay was used to detect the IL-6 and TNF-α level in supernatant of cell culture medium. The content of P-ERK/ERK, P-p38/p38, P-JNK/JNK, P-IKβ-α and P-p65 were detected by Western-blot method. The nuclear translocation of p65 was detected by immunofluorescence. Laser confocal scanning was used to observe the binding degree of LPS on Raw 264.7 cell membrane. Results: SNS inhibited, in a concentration dependent way, the differentiation of Raw 264.7 cells induced by LPS and the release of IL-6 and TNF-α. SNS also decreased the content of phosphorylated proteins such as P-ERK, P-p38, P-JNK, P-IKβ-α and P-p65 in MAPKs/NF-κB pathway, inhibited p65 translocation into nucleus and transcription, and blocked the binding of LPS with Raw 264.7 cell membrane. Conclusion:SNS could protect LPS-induced inflammation in Raw 264.7 cells, which may be related to its inhibition of phosphorylation of MAPKs/NF-κB pathway.
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  Research progress on factors influencing tendon-to-bone and methods promoting tendon-to-bone healing

  XIAO Lei, ZHENG Boyuan, ZHUANG Tengfeng, WANG Huajun, ZHENG Xiaofei

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 19-30. https://doi.org/10.11778/j.jdxb.2019.01.003

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  Through searching PubMed, CNKI(China National Knowledge Infrastructure), Wanfang Data and other databases, we found that the normal tendon-bone insertion point consists of four layers structure complex. the tendon-bone healing is affected by many factors.; there are many methods to promote tendon-bone healing, such as growth factors, cell-based therapy, gene therapy, and so on. However, most of the methods to promote tendon-bone healing are still in the animal experimental stage, and a large number of clinical studies are still needed to verify its effectiveness.
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  Effect of decitabine on the cell apoptosis and proliferation of ovarian cancer cell line SKOV3

  JING Shuang, JING Jiayu, HE Xiaoli, WANG Yue

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 31-36. https://doi.org/10.11778/j.jdxb.2019.01.004

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  Objective:To investigate the effects of decitabine on the cell apoptosis and cell proliferation of human ovarian cancer cell line SKOV3. Methods: The SKOV3 cells were treated with different concentrations(10, 100, 200 nmol/L)of decitabine. Flow cytometry was used to detect the effect of decitabine on cell apoptosis in SKOV3 cells. Cell counting kit-8 was used to detect the effect of decitabine, cisplatin and decitabine combined with cisplatin on cell proliferation in SKOV3 cells. Results: The inhibition effect decitabine at 100 nmol/L on the proliferation of SKOV3 cells was most obvious, and the growth inhibition rate was 61.31% (P<0.05). The inhibition rate was not significantly increased wtih decitabine over 100 nmol/L. Compared with decitabine or cisplatin alone, decitabine at low concertration combined with cisplatin caused a higher inhibition rate of cell proliforation (P<0.05). The apoptosis rate was increased in SKOV3 cells after the treatment with decitabine (P<0.05). Conclusion:Low dose decitabine promoted cell apoptosis and inhibied cell proliferation in SKOV3 cells. Low dose decitabine may prevent tumor development through enhancing the chemotherapy sensitivity of cisplatin. It provided a new idea for the treatment of drug-resistant ovarian cancer.
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  Evaluation of the effects of fireneedle on level of peripheral adenosine in postherpetic neuralgia rats by microdialysis

  LI Jingjing, ZHOU Peng, ZENG Jingchun, PEI Wenya, LIN Guohua

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 37-44. https://doi.org/10.11778/j.jdxb.2019.01.005

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  Objective:To investigate the mechanism and timeliness of different methods (Lingnan fireneedle and electroacupuncture) on neuralgia. Methods: Thirty-two male Sprague-Dawley (SD) rats were randomly divided into normal control group, model control group, electroacupuncture group and Lingnan fire needling group. 50 μL inoculation solution of herpes zoster virus was subcutaneously injected into the plantar area of the right hindpaw of rats to make the model of PHN. Real-time online monitoring was performed by using microdialysis to investigate the effect of fireneedle and electroacupuncture on concentration of peripheral adenosine and its metabolites in rats with postherpetic neuralgia. Results: The level of adenosine, AMP and ADP in peripheral tissues of rats in each group was decreased after modeling. At 30-60 minutes after treatment, the topical level of adenosine and AMP in the electroacupuncture group was close to the baseline level, which was of statistically significant difference from model group (P<0.01). The level of adenosine, AMP and ADP in the electroacupuncture group gradually decreased to the level in model group at 90 min after treatment (P>0.05). In Lingnan fireneedle group, the onset time of analgesic effect was 60 min after treatment, and the peripheral level of adenosine, AMP and ADP was still at baseline at 120 min after treatment, which had no significant difference from normal control group (P>0.05). There was statistical difference between electroacupuncture group and Lingnan fireneedle group (P<0.01). Conclusion:Electroacupuncture makes an analgesic effect from 30 min after treatment, while fireneedle from 60 min and lasts 2 hours at least. Lingnan fireneedle has the advantage of lasting analgesic effect when compared with electroacupuncture.
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  Correlation between DEXA measured adult body fat distribution and diabetes and cardiovascular disease

  WANG Yu, FENG Lie

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 45-51. https://doi.org/10.11778/j.jdxb.2019.01.006

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  Objective:The main purpose of this work is to measure adult body fat mass fraction and distribution by dual-energy X-ray absorptiometry (DEXA), to explore the relationship between body fat fraction and insulin resistance, islet beta cell function and metabolic disorder, and thus to reveal the correlation of body fat and type 2 diabetes mellitus and cardiovascular diseases. Methods: Type 2 diabetes mellitus (n=44)and non-diabetic patients(n=21)was recorded with clinical basic data including blood biochemical indicators, simple anthropometric parameters, total body fat mass fraction and distribution measured by DEXA, and multi-slice spiral CT coronary angiography. The baseline observation indexes were tested as well. Results: There was statistical significance (P<0.05) observed in diastolic pressure, blood sugar and C peptide 2 hours after meal, HbA1c, BMI, HOMA-β, coronary artery calcium score, body fat mass fraction between patients of diabetic group and non-diabetic group. The chief factors influencing coronary artery calcium score include TC (β=25.753,P<0.01), HDL-C (β=-32.954,P<0.01) and HbA1c (β=3.34,P=0.02), those influencing HOMA-TR were LDL-c (β=0.719,P=0.03) and BMI (β=2.619,P<0.01), and those influencing HOMA-β were LDL-c (β=78.934,P<0.01 and trunk fat content (β=3.87,P=0.02). Conclusion:The mass fraction and distribution of body fat have some effect on the function of islet cells and cardiovascular system.
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  Effect of cardiac resynchronization therapy on inflammatory cytokines in patients with chronic heart failure

  HOU Xiaolin, ZHOU Yongmei, TANG Yijia

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 52-56. https://doi.org/10.11778/j.jdxb.2019.01.007

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  Objective:To analyze the change of serum inflammatory cytokines TNF-α, IL-1 and IL-27 before and after cardiac resynchronization therapy(CRT)in patients with chronic heart failure (CHF). Methods: Seventy patients with chronic heart failure were divided into combined treatment group and simple drug group, 35 cases in each group. In simple drug group, the patients were treated only with the optimal drug therapy, while the patients were given the optimal drug therapy together with CRT in combined treatment group. The levels of TNF-α, IL-1, IL-27 and cardiac function indexes in patients before and after 3 months of treatment were recorded and compared. Results: After 3 months, both groups showed obvious decrease in TNF-α and IL-1 level and increase in IL-27 level, and the change in combined group was more significant than in simple drug group (P<0.05). The left ventricular end-diastolic dimension, left ventricular ejection fraction and left ventricular mass index in both groups were better than those before treatment, and the combined group was better than simple drug group with statistical significance (P<0.05). Conclusion: After CRT treatment, the level of inflammatory factors and cardiac function indexes in patients with CHF was improved. The level of TNF-α, IL-1 and IL-27 can be used as one of the indicators for evaluating the effect of CRT.
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  Prognostic value of neutrophil to lymphocyte ratio in patients with acute aortic dissection

  YU Liqiong, ZHANG Aidong

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 57-63. https://doi.org/10.11778/j.jdxb.2019.01.008

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  Objective:To investigate the prognostic value of neutrophil to lymphocyte ratio (NLR) in short- and long-term mortality risk of patients with acute aortic dissection (AAD). Methods: A total of 141 patients diagnosed with AAD were collected for clinic data retrospectively. The Patients were divided into death group (n=41) and survival group (n=100) according to whether there was a death event in 2-week follow-up. According to the quartile count of NLR, the patients were divided into Q1 group (NLR<4.33, n=35), Q2 group (4.33≤NLR<7.26, n=35), Q3 group (7.26≤NLR<11.78, n=36) and Q4 group (NLR≥11.78, n=35). Results: The NLR was 9.67 (6.81, 13.07) in deceased patients, which was significantly higher than that (6.18 (3.63, 11.10)) in in survived patients (P<0.05). Kaplan-Meier analysis showed that the cumulative short- and long-term mortality risk was greatly higher in patients with elevated admission Q4 levels when compared with those with low admission NLR in the other 3 groups. In multivariate Cox regression analysis, it showed that elevated admission NLR (≥11.28) in Q4 group remained an independent predictor of short-term mortality of AAD (HR to 28.403, 95% CI 3.392~237.865) and long-term mortality (HR to 3.396, 95% CI 1.596~7.228) (all P<0.05). Conclusion:Admission NLR is an independent predictor of short- and long-term mortality risk in patients with AAD. The higher NLR, the poorer prognosis.
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  Clinical analysis of hysteroscopy for difficult intrauterine devices (IUD) under local anesthesia

  LIU Yingwei, ZHAO Ling, CHEN Xing, DAI Xuelin, DENG Youlin

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 64-70. https://doi.org/10.11778/j.jdxb.2019.01.009

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  Objective:To explore the clinical significance of hysteroscopy in treating difficulty of removing intrauterine device (IUD). Methods: The clinical data of 202 cases of difficult IUD removal from January 2015 to December 2017 were retrospectively analyzed, and the causes and operation process were analyzed. Results: The 202 cases of failure of IUD removal from other hospitals was mainly caused by incarceration, rupture, residual or ectopic IUD with the diagnosis of hysteroscopy. In the 202 patients, IUD incarceration occurred chiefly in the metal ring or triangle IUD (113 cases, 55.9%) and “V” type MCu IUD (62 cases, 30.7%). The IUD were failed to be removed under hysteroscopy in 5 cases, while were successful in 197 cases (97.5%). There were 27 cases difficult to remove IUD due to abnormal uterus, 12 cases due to submucosal myoma and 15 cases due to uterine cavity/cervical adhesion. The operation time spending in the cases with rupture and residual IUD and the history of cervical operation is the longest. The bleeding volume in patients with submucosal myoma is the most, which is 32.5±3.4 mL. Conclusion:The metal round/triangle contraceptive device and V-shape metal ring are more likely to occur intrauterine incarceration. Submucosal myoma and uterine/cervical adhesion are common cause of difficult removal of IUD caused by abnormal uterus. The hysteroscopy surgery is a better way to remove intrauterine incarcerated contraceptive device which is difficult to be taken out by routine methods.
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  Microsurgical operation and gamma knife radiosurgery for medial sphenoid ridge meningioma

  DING Tao, ZHENG Maohua, ZHANG Fangcheng

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 71-78. https://doi.org/10.11778/j.jdxb.2019.01.010

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  Objective:To explore the effectiveness of microsurgery and Gamma knife radiosurgery for medial sphenoid ridge meningioma. Methods: 39 cases of medial sphenoid ridge meningioma, admitted into our department from March 2008 to December 2015 and undergoing operations, were retrospectively analyzed.30 cases were operated via pterional approach or improved pterional approach, 5 cases via subfrontal approach, 4 cases via frontotemporal-orbitozygomatic approach (FTOZA). Five cases with recurrence and 8 cases of incomplete resection and were treated with Gamma knife radiosurgery. Results: In this study, total resection (Simpson Grade Ⅰ or Ⅱ) was achieved in 69.2% (27/39); subtotal resection (Simpson Grade Ⅲ) in 20.5% (8/39); partial resection (Simpson Grade Ⅳ) in 10.3% (4/39). In 6 cases of recurrence, 5 cases received Gamma knife radiosurgery and the control rate was 60% (3 cases). In 12 cases of subtotal and partial resection (Simpson Grade Ⅲ and Ⅳ), 8 cases received Gamma knife radiosurgery and the control rate was 75% (6 cases). After follow up for 6 months to 8 years (mean 45 months), the prognosis was excellent in 32 cases, general in 4 cases and poor in 3 cases, and one patient died. In 21 patients with visual field disorder before operation, 7 had marked improvement in visual acuity after operation, 9 showed no obvious change and 5 got worse. In 7 patients with preoperative hemiplegia and hemidysesthesia, 6 were improved and 1 aggravated postoperatively. In 6 patients with preoperative oculomotor nerve palsy, 1 patient was significantly improved, 2 had no apparent change and 3 worsened postoperatively. After operation, there were 6 new cases of oculomotor nerve palsy, 3 of conscious disturbance, 1 of contralateral hemiplegia, 1 of language dysfunction, 1 of mental disorder and 1 of anosphrasia. Conclusion:Whether performing total resection of medial sphenoid ridge meningioma depends on the position, size, texture and surrounding edema degree of tumor and the relationship of tumor with cavernous sinus and internal carotid artery. The application of CUSA and plasma knife can improve total resection rate of tumor. Gamma knife radiosurgery is a safe and effective method for treating tumor residue and recurrence after operation.
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  Value of IVIM-DWI in diagnosis of peripheral zone prostate cancer

  DU Shuman, ZHANG Lin

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 79-84. https://doi.org/10.11778/j.jdxb.2019.01.011

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  Objective:To investigate the diagnostic value of quantitative parameters of 3.0 TMR intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI) in diagnosis of peripheral zone prostate cancer (PCa). Methods: The data of 51 patients with peripheral zone prostatic lesions confirmed by ultrasound-guided biopsy were retrospectively analyzed, including 27 patients with prostate cancer (malignant group), 36 patients with benign prostatic hyperplasia (BPH) and/or chronic prostatitis (CP) patients (benign group). Routine MRI and IVIM-DWI were performed in all patients. IVIM-DWI was performed with 14 b values (b=0, 25, 50, 75, 100, 150, 200, 400, 600, 800, 1 000, 1 200, 1 500, 2 000 s/mm²). GE ADW4.6 Functool post-processing software was used to calculate the apparent diffusion coefficient (ADC), pure water molecular diffusion coefficient (D), perfusion-related diffusion coefficient (D^* value) and perfusion fraction (f value) of the region of interest in the lesion. Independent sample t test was used to compare the differences of parameters between PCa group and BPH group. For the parameters with statistical significance between the two groups, the work characteristic curve of subjects was established to analyze the diagnostic efficacy of each parameter value for prostate cancer. Results: The ADC value, D value and F value of group PCa were significantly lower than those of BPH group (P<0.05). There was no significant difference in D^* between groups (P=0.581). Conclusion:The ADC and D values of IVIM are of high value in the diagnosis and differential diagnosis of benign and malignant peripheral prostate lesions, and the ADC value is the best.
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  The application of multi-neighborhood median filterin medical images

  CHEN Jiayi, DONG Mengyi, ZHAN Yinwei, CAO Huiying, XIONG Gangqiang

  Journal of Jinan University Natural Science & Medicine Edition. 2019, 40(1): 85-94. https://doi.org/10.11778/j.jdxb.2019.01.012

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  Objective: To overcome the drawbacks of existing filters in noise detection and removal, and further improve the denoising performance. Methods: We propose a multi-neighborhood median filter to improve the techniques of noise detection and removal.This proposed filter first performs noise detection by the extreme intensity values of neighborhood,and then performs further noise detection by the median of neighborhood. For noisy pixel, it searches for the medians of nine neighboring neighborhood respectively, and then take the median of all medians as the new intensity of noisy pixel. Results: The experimental results with medical images,show that compared to the existing filters, the propose method obtains clearer denoised images, and achieves higher PSNR and SSIM values. Conclusion: The proposed method can preserve the edges and details of image very well, while removing the noises thoroughly; comparing to the existing filters, the proposed method shows better denoising performance.