Journal of Jinan University Natural Science & Medicine Edition

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BDNF involved in the protection of microvascular endothelial cell apoptosis under glucose starvation

YAO Yuechun, CAI Dongqing, CHEN Yilin

Journal of Jinan University Natural Science & Medicine Edition, 2023, 44(3): 238-247. DOI: 10.11778/j.jdxb.20220356

Figure 4 The influence of inhibition of BDNF expression in apoptosis of microvascular endothelial cells under glucose starvation A: The influence of siBDNF-teated in the expression of BDNF mRNA in bEnd.3 cells; B: The influence of siBDNF-teated in the expression of BDNF mRNA in bEnd.3 cells under 6-hour glucose starvation; C: The flow cytometry scatter plots of apoptosis of siBDNF-treated bEnd.3 cells under 24-hour glucose starvation; D: Quantitative analysis of C results. 1)P<0.05; 2)P<0.01; 3)P<0.001; 4)P<0.0001.

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Figure 1 The influence of glucose starvation in apoptosis of microvascular endothelial cells A: CD31 positive rate of bEnd.3 cells; B: The situation of bEnd.3 cells to uptake DiI-Ac-LDL; C: The relationship of bEnd.3 cell death with the time of glucose starvation; D: The flow cytometry scatter plots of bEnd.3 cell apoptosis at different time points of glucose starvation; E: The result of bEnd.3 cell apoptosis in different time points of glucose starvation. 1)P<0.05; 2)P<0.001; 3)P<0.0001.
Figure 2 The influence of glucose starvation in the BDNF expression in microvascular endothelial cells A: The influence of glucose starvation on BDNF mRNA level in bEnd.3 cells; B: The influence of glucose starvation on BDNF protein level in bEnd.3 cells. 1)P<0.01; 2)P<0.001
Figure 3 The influence of inhibition of BDNF-TrkB pathway in the early apoptosis of microvascular endothelial cells under glucose starvation A: The flow cytometry scatter plots of apoptosis of K252a (blocker of BDNF pathway) treated bEnd.3 cells under 48-hour glucose starvation; B: Quantitative analysis of A results. 1)P<0.01.
Figure 5 The influence of BDNF in the expression level of PFKM gene A: The influence of siBDNF treatment in the expression of HK2 gene mRNA (a key regulation gene of glycolysis) in bEnd.3 cells under non-glucose starvation and glucose starvation; B: The influence of siBDNF treatment in the expression of PFKM gene mRNA (a key regulation gene of glycolysis) in bEnd.3 cells under non-glucose starvation and glucose starvation. 1)P<0.05; 2)P<0.01; 3)P<0.001.

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