Journal of Jinan University Natural Science & Medicine Edition

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Effects and mechanism of Hanjingtang on phenotype conversion and inflammation and oxidative stress in cold coagulation hypertensive rats

LIU Yang, CHEN Guihai

Journal of Jinan University Natural Science & Medicine Edition, 2023, 44(4): 347-357. DOI: 10.11778/j.jdxb.20230010

Figure 5 Expression levels of SOD and MDA in rat serum of each group A:Expression level of serum SOD in each group of rats; B:Expression level of serum MDA in each group of rats.1)Compared with CON group, P<0.01;2)Compared with MOD group, P<0.01.

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Figure 1 Changes in blood pressure of rats in each group A: Change in systolic blood pressure in each group of rats($\bar{x}±s$,n=9); B: Change in diastolic blood pressure in rats of each group($\bar{x}±s$, n=9). 1)Compared with CON group, P<0.05; 2)Compared with CON group, P<0.01;3)Compared with model group, P<0.01.
Figure 2 HE staining results of rat aorta (×200)
Figure 3 Masson staining results and collagen fiber expression levels in rat aorta A:Masson staining results of rat aorta in each group(×200); B:Collagen fiber levels of rat aorta in each group.1)Compared with CON group, P<0.01;2)Compared with MOD group, P<0.01.
Figure 4 Comparison of the protein levels of α-SMA,OPN,and calponin in the rat aorta A:Immunohistochemistry assay result of α-SMA in rat aorta of each group(×200);B:Immunohistochemistry assay result of OPN in rat aorta of each group (×200);C:Immunohistochemistry assay result of calponin in rat aorta of each group (×200);D:Expression of α-SMA, OPN and calponin protein levels in rat aorta of each group.1)Compared with CON group,P<0.01;2)Compared with MOD group,P<0.05;3)Compared with MOD group,P<0.01.
Figure 6 Expression levels of IL-1β, TNF-α, and NLRP 3 in rat serum of each group A:Expression level of serum IL-1β in each group of rats;B:Expression level of serum TNF-α in each group of rats; C:Expression level of serum NLRP3 in each group of rats.1)Compared with CON group, P<0.01;2)Compared with MOD groups, P<0.05; 3)Compared with MOD groups, P<0.01.
Figure 7 Expression of Nrf2, NQO1, NF-κB and IκBα proteins in rat aortic tissues of each group analyzed by Western blot A: Band diagram of protein expression in rat aorta of each group;B: Relative protein expression in rat aorta of each group.1)Compared with CON group,P<0.01;2)Compared with MOD group,P<0.05;3)Compared with MOD group,P<0.01.
Figure 8 The expression of NQO1 and NF-κB genes in the rat aortic tissues was analyzed by Rt-PCR 1)Compared with CON group, P<0.01; Compared with MOD group, 2)P<0.05, 3)P<0.01.

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